dna binding domain 中文意思是什麼
dna binding domain
解釋
dna結合性結構域-
Sox genes encode a group of proteins that carry a dna - binding hmg domain
Sox基因含有一個能與dna特異結合的高度保守hmg - box基序。 -
The env protein deduced from env gene encodes the hydrophilic surface protein ( su ) and the hydrophobic transmembrane domain ( tm ) that determine the specific interaction between virus particles and cell surface receptors during retroviral entry. the su of retroviruses is a highly variable genetic element, containing receptor binding sites and major antigenic determinants. exjsrv - specific dna probes were derived. by using these dna probes in tissue hybridization. we successfully identified jsrv mrna expression and proviruses dna in sheep lung tissues infected with jsrv and control group has no postive signals, validating the use of exogenous virus - specific dna probes in the analysis of oncogenic proviral integration sites and identification of integrated exogenous proviral sequences
用地高辛隨機引物法標記exjsrv特異的env片段，制備探針，原位雜交檢測spa肺組織中的rna及前病毒dna ，結果表明spa患羊肺組織內有jsrvenv基因mrna的表達，同時也檢測到了前病毒dna ，而相應的陰性對照卻無陽性信號，證實外源性病毒特異的dna探針在致瘤性前病毒的整合位點和整合的外源性前病毒的檢測中具有可信度。 -
Ie acts as a general gene transcription activator. it consists of dna - binding domain and acid amino acid transcription activate domain. what kinds of promoter can be stimulated by ie 180
另外ie180與hsv的立即早期蛋白icp4有較大的同源性，研究ie180轉錄調控作用有利於皰疹病毒轉錄調控模型的建立。 -
By elisa analysis, inhibition of binding of clq with the c ! q receptors on u937 cells and competitive inhibition of binding of clq with aggregated immunoglobulin g b y selected phage clones, and dna sequencing, a number of similar, but not identical, sets of sequences of clq - binding clones were identified. the deduced amino acid sequences of selected 9 peptides are wyegpftlytwp, hwdpfslsayfp, ltqhnspffllp, tsnpfflwypqp, qtpfqlw, npfnwts, spfxlts, fltwldp and fstflyp. they show significant efficiency to inhibit the binding of clq with the clq receptors on u937 cells and / or aggregated immunoglobulin g, which suggest that the selected peptides contain the modeling epitopes of clq receptor to bind the collage - like region or igg to bind the head domain of clq
然後，採用噬菌體肽庫技術，以c1q為釣餌蛋白，從12肽庫和環7肽庫中親和篩選能與c1q結合的噬菌體克隆，經elisa 、 u937細胞配體結合抑制試驗、 aigg競爭抑制試驗及dna測序，獲得了9個具c1q抑制活性克隆的dna序列，其相應的氨基酸序列為： wyegpftlytwp 、 hwdpfslsayfp 、 ltqhnspffllp 、 tsnpfflwypqp 、 qtpfqlw 、 npfnwts 、 spfxlts 、 fltwldp 、 fstflyp ，它們可能模擬c1qr和或igg的c1q結合表位並抑制c1q的活性。 -
The gpa1 gene was obtained via pcr amplification and was cloned in the two - hybrid dna binding domain vector pgbkt7 the combinant plasmid was designated as pgbkt7 - ga. - galactosidase assay indicated that got did not have the property of self - activation. after pgbkt7 - ga was transformed to yeast pj69 - 4a, we transformed arabidopsis vegetative tissue two - hybrid cdna library plasmids to yeast pj69 - 4a containing pgbkt7 - ga
通過pcr擴增得到gpa1基因並將其克隆到雙雜交dna結合域載體pgbkt7中，得到的重組質粒命名為pgbkt7 - g ， ?半乳糖苷酶活性鑒定表明g不具有自激活特性，將其轉化到酵母菌pj69 - 4a中，再以此為受體菌轉化擬南芥綠色營養組織cdna文庫質粒。 -
The predicated amino acid sequences contain two conserved functional domains : a dna - binding domain ( hmg - box ) and a transactivation domain. the conserved motif of group b homology lies immediate adjacent to c - proximal region of the hmg - box. similar structure and identity of sox2 gene among mammals, birds, amphibians and reptiles suggest that sox2 gene have evolutionary conserved roles
進一步分析表明，已巴灘2編碼的238個氨基酸多膚鏈含有兩個保守的功能區域:位於n端的hmg盒區（ 1一78aa ）和位於c端的轉錄激活區（ 131一236aa ） ，以及一個保守的特徵性b亞族同源區域（ 79一90aa ）與hmg盒區的c端相連。 -
It is show the mutant consists of a nuclei binding site and dna - binding domain is enough to negative regulation. a report plasmid pcat that we constructed has a cat gene expressed by a cmv early promoter
（ 465 1079氨基酸段）對sv40啟動子明顯的抑制活性，因為它只包含dna結合域和核結合位點，說明發揮轉錄抑制功能的關鍵是ie180的dna結合域。 -
2. a bhlh - type gene osbhlhl was obtained from the rice cdna library by using the rapid cloning pcr. the predicted osbhlhl protein had a nucleus location signal, a putative dna binding domain contained a bhlh - bzip domain
2利用pcr篩庫法，從水稻cdna文庫中克隆到一個bhlh類型的基因，命名為osbhlh1 ，它的推測蛋白結構中含有一個細胞核定位信號和一個可能的與dna結合bhlh - zip結構域。
分享友人