dna extraction 中文意思是什麼
dna extraction
解釋
dna提取- dna : 1. deoxyribonucleic acid 【生物化學】去[脫]氧核糖核酸。2. deoxypentose-nucleic acid 去[脫]氧戊糖核酸。
- extraction : n. 1. 抽出，拔出。2. 【化學】提取（法）；萃取（法）；回收物，提出物；精煉。3. 精選，摘要。4. 血統，家世，出身。5. 【數學】開方，求根。
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The respiratory intensity of the contaminated soil decreased by 29. 93 % while ammonification and nitrification increased significantly than that of control soil. 2. extraction and purification of soil microbial total dna a method of extracting soil total dna was developed, and it can extract dna from g + bacteria
二、土壤微生物總dna的提取和純化方法研究為了採用不依賴于培養的16srdna分析的方法研究有機磷農藥長期污染對土壤微生物群落結構的影響，建立了從土壤中提取總dna的方法，並通過改進使適合於對革蘭氏陽性菌的提取。 -
A simple and rapid method for genome dna extraction from arabidopsis
以氣相色譜法 -
Among the three methods used in the experiment of dna extraction, only ctab, adding pvp in the dna isolation step, had effectively reduced the disturbance from fiber or other plastids and extracted suitable genomic dna as template for rapd process. pcr amplifications were performed in a final volume of 25 mm3 containing 0. 5 units taq polymerase, 2
通過在抽提緩沖液加入2的pvp 、並用異丙醇和乙醇沉澱基因組dna等改良措施， ctab法能避開大量纖維、多糖的影響，有效地從不同屬種的棕櫚科植物的幼嫩葉片中提取並純化了適合rapd的基因組dna 。 -
Dna extraction from different specimens is the foundation of conservation genetics. it is difficult to extract total dna from tannage of chinese alligator with usual method
利用不同的實驗材料提取適合研究的dna是保護遺傳學研究的基礎，而對于鞣製皮革，用常規的dna提取方法很難提到適合於研究的dna樣品。 -
In this paper, total dna from tannage, tail skin, scales and the skin treated with salt were successfully extracted with an improved method for dna extraction. to verify the results, four pairs of primers, which were universal primers for 12s rrna gene, diagnostic primers of chinese alligator meat, microsatellite primers and rapd primer, were used to do pcr amplifications. some amplified fragments were sequenced, either
本文研究出一種改進的dna提取方法，成功地從揚子鱷鞣製皮革中提取了總dna ，同時對不同的組織標本如鱗片、鹽腌生皮及尾尖皮等進行了dna提取：並利用12srrna基因擴增的通用引物、揚子鱷鑒定性引物、微衛星引物及rapd引物進行pcr擴增，且對部分pcr擴增結果進行測序，以檢驗提取效果。 -
Two plasmids, which contain hbv specific dna fragment and hsv1 dna fragment, were amplified by solid phase two loci pcr and detected by enzymatic indicator system on a gene chip that was constructed by primer immobilization and modified with thiol group on chip surface. for building detection technology by dna chip in clinical, the virus genomes were extracted from the clinical positive samples by one - step nucleic acid extraction
採用已建立的晶元制備方法，在該六種質粒中選擇含有hbv與hsv1兩種病毒dna序列的質粒為模板，進行同相兩重pcr ，採用晶元酶學槍測對固相兩重pcr的擴增結果進行檢測，得到良好的晶元酶學檢測結果。 -
Extraction of large - fragment genomic dna in order to gain dna template of pcr amplification ( long pcr amplification and salvage pcr amplification ) which was high purity and large fragment, three methods were used to extract genomic dna of bacillus subtilis, i. e. low melting - point agarose embedding method, sds - proteinase k - phenol chloroform extraction method and bacterial genomic dna extraction kit method. the genomic dna of bacillus subtilis were gained by these methods, and the operated programs of the methods were improved. the results showed that the genomic dna extracted by low melting - point agarose embedding method were obviously biggest than that of another two methods
大片段基因組dna的提取為了獲得用於pcr擴增（長距離pcr擴增和分段pcr擴增）的高純度、大片段（至少為pcr產物長度的4倍）的dna模板，應用三種方法：低熔點瓊脂糖包埋法， sds -蛋白酶k -酚氯仿抽提法和細菌基因組dna提取試劑盒法，分別提取獲得了枯草桿菌基因組dna ，並對3種方法的操作程序進行了不同程度的改進，結果表明：低熔點瓊脂糖包埋法提取的基因組dna片段明顯大於后兩種方法，採用0 . 5瓊脂糖凝膠電泳3h ，仍然跑不出加樣孔。 -
Materials and methods in our study, first the e. coli bl21 transformed already are cultivated in smaller scale and then the plasmids dna were extracted by the methods of alkaline lysis. the plasmids dna extraction were processed 37 overnight by the restrictive endo - incisase bam h i and xho i. the incision products were used to check the integrality and variability of the recombinant plasmids dna by 1 % agarose gel elec - trophoresis
同預想結果基本吻合，大規模培養后純化得到的融合蛋白通過sds page顯示在52kda處有一條帶，而酶切后產物電泳結果顯示在26kda處分另有兩條蛋白帶，其中一條為gst ，另一條為hbrp ，基本符合實驗結果； hbrp對tpk活性的抑制呈劑量依賴性。 -
This dissertation mainly focuses on studying these three parts. 1. two types of dna purification chips based on the method of spe ( solid phase extraction ) have been fabricated and studied
其主要技術是製作微流路的生物晶元（ microfluidicbiochip ） ，針對這些，本文主要的研究內容如下： 1 -
The plasmid extraction from wild strains needs an appropriate method. first, lysis by alkali was used to extract plasmid from strain pseudomonas xn - 1. but the expected result had not been got, and a lot of cracked plasmid dna fragments were got only
對於野生菌株的質粒提取，需要選擇合適的提取方法，我們首先採用了細菌細胞質粒提取常用的堿裂解法，但是未取得理想的效果，只得到了許多破碎的質粒dna片段。 -
2 ) the method of proteinase k isolation and the method of ctab isolation had been compared carefully while extracting the genome dna from crickets. the result showed the method of proteinase k isolation of total dna was more suitable for the extraction of total dna from insects of grylloidea
2 ）在提取蟋蟀總科昆蟲的基因組dna時，比較了ctab法和蛋白酶k法兩種方法，結果顯示蛋白酶法更適用於蟋蟀總科昆蟲的基因組則a的提取。 -
4. the improved method of ctab isolation of total dna is suitable for extraction of total dna from insects of troides
首次採用改進的ctab法提取裳鳳蝶基因組dna ，所提樣品完全可以滿足rapd及線粒體dna的擴增。 -
The dissertation consists of five chapters : in chapter one, the recent progress in molecular approaches in systematic studies of macroalgae e. g. dna extraction, restriction endonuclease fragment length polymorphisms ( rflps ), random amplified polymorphic dna ( rapd ), gene sequencing, intersimple sequence jepeats ( issr ), amplified fragment length polymorphisms ( aflp ) and single strand. conformation polymorphisms ( sscp ) were reviewed
本論文由五部分組成：在第一部分，綜述了大型海藻dna的提取、限制性片段長度多態性（ rflps ） 、隨機擴增多態性dna （ rapd ） 、核酸序列分析、擴增片段長度多態（ aflp ） 、單鏈構象多態（ sscp ）等分子手段在大型海藻系統學研究中應用的一些進展。 -
After electrophorised on 1 % agarose gel, the pcr production was purified with agarose gel dna extraction kit. the segment was ligated with vector pmd18 - t and then was tranformed into the competent cell of dh5 a. a construction mstnd - pmd18t was generated by inserting the sequence of 254bp into pmd18 - t vector and selecting the sense clones. positive clone was identified by three ways : endonuclease digestion, pcr and sequencing. the result showed that the cloned sequence coincides with the designed sequence. this construction was digested with nco i and xho i and ligated the pet28a ( + ) vector digested with the same enzymes using dna ligation kit. the production of ligation reaction was transformed into the competent cell of bl21 ( de3 ). after 12 - 16 hours of culture, several colnes appeared on the plate. some positive clones were selected to extract their plasmid. these plasmids were digested by nco i and xho i and indentified by pcr. a contraction, mstnd - pet28a was generated. the result showed that the cloned sequence coincides with the designed sequence
F _ 1長38bp ， r _ 1長36bp ，其它片段均40bp長， f _ 1和r _ 1片段兩端分別加上限制性內切酶nco和xho的識別位點序列。用成對單鏈片段進行延伸反應，然後用其他單鏈片段作為引物，進行pcr擴增，用dna快速純化回收試劑盒回收所得254bppcr產物，與pmd18 - t載體連接、轉化dh _ 5 。受體菌感受態細胞，利用藍白斑遺傳學篩選法篩選陽性克隆，提取其質粒，採用nco和xho雙酶切鑒定，獲得了254bp的片段；用pmd18 - t載體上的特異引物rv - m和m13 - 47進行pcr鑒定，獲得300bp的片段。 -
One small captive population of amur tigers ( panthera tigris altica ), which had 14 - year captive history since its foundation in 1986, was selected for such population genetic researches in this paper, which contained three experimental parts as : ( 1 ) dna extraction from shed hairs. three methods, which were chelex, ionic detergent and non - ionic detergent, were respectively used to digest the shed hairs of one amur tiger
本文利用chelex 、離子型和無離子型分解液三種方法對在東北虎（ pantheratigrtsaltica ）換毛期採集的自然脫落毛發進行了dna提取，並對部分毛發分解原液進行蛋白質沉澱，對于毛發分解殘留物也進行了核細胞分解與蛋白質沉澱再處理，對上述提取產物進行rapd擴增。 -
Comparison research of methods of dna extraction from grape
提取方法的比較研究 -
Comparison of different dna extraction methods for activated sludge
不同提取方法的比較 -
After plasmid and cherosome dna extraction and purification, the gyra of 16 isolates of salmonella gene was amplified by polymerase chain reaction ( pcr )
測定了16株致病性沙門氏菌對12種抗菌藥物的最低抑菌濃度（ mic ） ，依照美國臨床實驗室標準委員會（ nccls ）標準（ 1990 ）判定菌株耐藥性。 -
The shotgun method was only used with b. subtilis hn503. the optimun conditions for total dna extraction, enzyme digest, ligation and transformation were studied the results showed that the most important factor was transformation efficiency. since the concentration of ligation products was low in common
在已知基因序列的條件下用pcr和rt一cr克隆基因時，對pcr和rt一pcr的反應條件、產物的回收、回收產物的酶切、酶連和轉化等條件進行了一系列的比較研究。 -
Hisityl - trna synthetase catalyzes the aminoacylation of trnahis in the initial step of protein biosynthesis. the involumen of histidyl - trna synthetase in autoimmune diseases is another feature of the enzyme. in studies, it is reported that purified jo - 1 antigen can increase the detection rate of anti - jo - 1 antibody. but it is difficult to obtain a single component by biochemical extraction. genetic engineering can help us to desolve this problem. after looking up mrna sequence encoding histidyl - trna synthetase in genebank, we used rt - pcr technology to gain its full length dna sequence. the vestor ptybllwas used in the construction of expressing vestor. we transformed the jo - 1 gene into er2566 and used this system to express fusion jo - 1 antigen
本實驗從人胎盤中提取總rna ，利用rt - pcr技術獲得了編碼jo - 1的基因整長序列，選用impact - cn系統中的ptyb11載體，構建了jo - 1基因的克隆與表達載體，並轉化大腸桿菌er2566 ，經過抗性篩選、分子量大小比較、雙酶切鑒定、和pcr鑒定等多種方法驗證，篩選出了5個陽性克隆。
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