dna fragment (length) 中文意思是什麼

dna fragment (length) 解釋
dna片斷（長度）
  • dna : 1. deoxyribonucleic acid 【生物化學】去[脫]氧核糖核酸。2. deoxypentose-nucleic acid 去[脫]氧戊糖核酸。
  • fragment : n. 1. 碎屑，碎片，破片，斷片。2. 未完稿，斷簡殘篇。vi. ,vt. （使）成碎片，（使）分裂。
  • length : n. 1. 長，長度，長短。2. （時間的）長短，期間。3. （賽艇的）一艇的長度；一馬的長度。4. 程度，范圍。5. 【板球】球程；投至適當距離的球。6. 【語言學】音長。7. 一段，一節。
  1. In the present study, aflp ( amplified fragment length polymorphism ) markers was used to examine population of abies yuanbaoshanensis in order to understand the level of population genetic variation and genetic structure. the result would help to evaluate its evolutionary potentiality and the degree of being endangered and could provide scientific basis for making right protection strategy. high - quality dna was extracted using ctab method from those tender leaves of forty - three fully - developed trees in population abies yuanbaoshanensis

    本研究選用一種高效的檢測遺傳變異的分子標記? ? aflp技術來分析元寶山冷杉種群的遺傳多樣性，旨在了解該種群在分佈區內的遺傳變異水平和遺傳結構情況；研究結果將有助於更清楚地認識這一瀕危類群的生存潛力和瀕危程度，而且可以為制定何種挽救和保護措施提供科學的依據。
  2. Mouse ; restriction fragment length polymorphism ; dna fingerprint ; probe jl - 02 ; es cell

    小鼠限制性片段長度多態性dna指紋圖jl - 02探針胚胎幹細胞
  3. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  4. 1. because the taxonomic division is rather complex and has been much disputed and revised, in this part, we will review the classification and phylogeny of families, subfamilies and tribes of anseriformes based on morphology, ethology, osteology, mitochondrial and nuclear dna restriction fragment length polymorphism, single - copy nuclear dna hybridization and the sequences of mitochondrial gene analysis referring to the different definition, classification and phylogenetic relationships of the families, subfamilies and tribes of anseriformes. the controversial questions and deficiency in the systematic studies of anseriformes were pointed out

    具體包括以下幾個部分： 1 、針對雁形目鳥類異常復雜的分類狀況及分類上存在的爭議，根據雁形目鳥類的形態學、行為學、骨骼學、角蛋白、線粒體與核dna酶切片段長度多態、單拷貝核dna - dna雜交及線粒體基因dna序列分析等方面的研究，對雁形目鳥類分類中科、亞科和族的劃分及其相互間的系統發生關系進行綜述，分析系統學研究中存在的不足，提出了雁形目鳥類分類中急需解決的問題。
  5. The activity of sod could increase as the longer of storage time no matter the samples covered with vegetable oil or not, kept in light or in dark. in the pcr reaction, a dna fragment with about 550pb length has been amplified by using the genomic dna of s. maltophilia 276 as the temperate, and by using the sequ

    根據報道的不同細菌的mnsod基因序列，以5 』一tacgaygcsctggarccg弓』序列作為上游引物，以5 』一ccagttcaccacgttcca於3 』序列作為下游引物，利用pcr反應從及胭1t叩hj1z 』 a276菌株的基因組dna中擴增出一條長度約為550bp的側a片段。
  6. Amplified fragment length polymorphisms ( aflps ), random amplified polymorphic dna ( rapd ) and microsatellite markers were used for genome mapping in the pacific oyster, crassostrea gigas thunberg

    本研究利用aflp 、 rapd和微衛星標記，以一個回交家系[ （ miyagixhiroshima ） xmiyagi ]為作圖群體，構建了長牡蠣的遺傳連鎖圖譜。
  7. The dissertation consists of five chapters : in chapter one, the recent progress in molecular approaches in systematic studies of macroalgae e. g. dna extraction, restriction endonuclease fragment length polymorphisms ( rflps ), random amplified polymorphic dna ( rapd ), gene sequencing, intersimple sequence jepeats ( issr ), amplified fragment length polymorphisms ( aflp ) and single strand. conformation polymorphisms ( sscp ) were reviewed

    本論文由五部分組成：在第一部分，綜述了大型海藻dna的提取、限制性片段長度多態性（ rflps ） 、隨機擴增多態性dna （ rapd ） 、核酸序列分析、擴增片段長度多態（ aflp ） 、單鏈構象多態（ sscp ）等分子手段在大型海藻系統學研究中應用的一些進展。
  8. The hinf i restriction enzyme digestion gave rise to restriction fragment length polymorphism ( rflp ) of the pcr products. when there were two dna fragments of 363bp and 305bp were produced from a pcr product, the strains were assumed to have a mutation at ser83, when three fragments of 363bp, 206bp and 99bp were produced, the strains were assumed to have no mutation at the 83or 116. the results indicated that certain mutation of ser 83 abolished a hinf i restriction enzyme site and may be detected as a rflp

    本研究重點分析了16株菌對氟喹諾酮類（ fqns ）藥物的耐藥性，結果表明： 16株菌對諾氟沙星、環丙沙星、沙拉沙星、單諾沙星和氧氟沙星等（ nor 、 cip 、 sar 、 dan 、 ofl ）的耐藥率在31 . 3 - 56 . 3之間。
  9. The aflp ( amplified fragment length polymorphism ) technique is based on the selective pcr amplification of restriction fragments from genomic dna digested by restriction enzymes completely

    擴增片段長度多態性技術（ aflp ）是基於選擇性擴增完全酶切消化后的基因組dna片段。
  10. Rapd ( random amplified polymorphic dna ), which bases on the polymerase chain reaction ( pcr ), is by far one of the most commonly molecular techniques to uncover dna sequence polymorphisms. the basic priciple of this technique is that an arbitrary primer ( usually lobp oligonudetide ) is used to amplify random segments of dna, and a small number of fragments will be amplified when the primer anneals on each strand over a length range. if sequence variation is present at the priming site, then a fragment may not be amplied, so the dna polymorphic can be detected

    Rapd （隨機擴增多態性dna ）技術是二十世紀90年代發展起來的一項dna分子多態性檢測技術，它建立於聚合酶鏈式反應（ pcr ）技術基礎之上，利用隨機合成的寡聚核苷酸序列為引物（一般為10個bp ） ，分別與dna的兩條單鏈結合，在dna聚合酶的作用下，對基因組的特定區域進行pcr擴增，其電泳結果為不同大小和數目的dna譜帶即rapd圖譜，可反映基因組相應區域的dna多態性。
  11. Distributed function of dna fragment length was also obtained, and fitted with tsallis entropy statistical theory. results show that high - let heavy ion radiation more effectively induces dna dsbs in comparison with low - let radiation, and produces dsbs that are distributed more locally and more densely

    結果表明，具有高let的重離子輻射與低let輻射相比，能夠更有效的誘發dna分子發生雙鏈斷裂，並使雙鏈斷裂的分佈更局部和更密集。
  12. Some characteristics of cold - active protease and chitinase were analyzed then. microbial 16s rdna ( ribosomal dna ) clone libraries of deep sea sediments were constructed and studied by pcr - rflp ( restriction fragment length polymorphism ) and phylogenetic analysis based on 16s rdna sequences. the microbial diversity and community structures of deep sea sediments collected from two different sea area including the west pacific " warm pool " and the east pacific " manganese nodule " area, as well as the interaction between microbial community and environment, were analyzed based on these studies

    通過構建沉積物中微生物16srdna克隆文庫，採用pcr - rflp分析、 dna - dna雜交、 16srdna序列測定以及系統發育分析的方法，研究了兩太平洋「暖池」區和東太平洋「結核」區兩個不同海區深海沉積物中的微生物多樣性和群落結構特徵及其與環境的相互關系，得到了一些與「暖池」區環境特點緊密相關的新發現，新認識。
  13. Construction, preparation and quantity of cea dna vaccine : plasmid pgem4 - cea containing full - length of cea cdna was cleaved by ecori, 2. 4kb fragment was recovered, and ligated with ecori - cleaved plasmid pci - neo

    Pci n的構建制備和定量： ecori酶切含仍a全基因的質粒pgem 。 cea ，瓊脂糖電泳回收2
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