dna p 中文意思是什麼

dna p 解釋
脫氧核糖核酸聚合酶
  • dna : 1. deoxyribonucleic acid 【生物化學】去[脫]氧核糖核酸。2. deoxypentose-nucleic acid 去[脫]氧戊糖核酸。
  • p :
  1. With the improved method of ctab, the genomic dna extracted form plum and other species close to p. mume in genetic relationship could be directly used in pcr amplification, with good results obtained

    選用改良ctab法提取的梅及其近緣種的基因組dna可直接用於pcr擴增分析，且效果良好。
  2. Cluster analysis by upgma methods indicated that these five stocks might be divided into three clusters. results of rapd a nalysis suggested that extensive genetic diversity was detected in this species and the genetic divergence among stocks was relatively high ; upgma phylogenetic tree showed there existed three geographic populations of p. polyactis in the yellow sea and the east china sea, which supported the previous conclusion by morphological and ecological methods. part two : the genetic diversity between trichiurus lepturus and eupleurogrammus muticus random amplified polymorphic dna ( rapd ) technique was applied to 12 individuals from each species of the hairtail fishes trichiurus lepturus and eupleurogrammus muticus collected from the yellow sea

    50一2 . 44 ） ，群體內和群體間的遺傳變異比例分別為69 %和31 % ;群體間的平均遺傳相似度和遺傳距離分別為0 . 9139和o . q861 ;用非加權配對算數平均法（ unweightedpair - groupmethodofari山m七tiome即s ， upgma ）聚類分析的結果表明，所分析的5個群體可被分為3個地理群系，從分子水平上支持了過去有關學者把黃海和東海的小黃魚劃分為北中南3個地理群系的觀點。
  3. Sedegah m, hedstrom rc, hobart p, et al. protection against malaria by immunization with plasmid dna encoding circumsporozoite protein [ j ]. proc natl acad sci usa, 1994, 91 ( 21 ) : 9866

    劉彥文，余新炳，徐勁等.惡性瘧原蟲海南株環子孢子蛋白基因的克隆與表達[ j ] .中國人獸共患病雜志， 2000 ， 16 （ 1 ） : 8
  4. Random amplified polymorphic dna ( rapd ) markers were used to construct molecular genetic linkage map of a single tree ( p. taiwanensis hayata. )

    採用rapd分子標記來構建黃山松單株樹（仙居磐黃）的分子遺傳連鎖圖譜。
  5. P and pr have relationships with spermatogensis and sperm maturation. 6. the product of c - fos translocates from the cytoplasm of spermatogonia to nuclei of spermatogonia, then it induces the transcription of dna and regulate spermatogonial proliferation and spermatogenesis

    C fos產物從精原細胞胞質中轉移到胞核中后通過啟動dna的轉錄而調控精原細胞的增殖，從而促進精子發生的進程。
  6. To search proteins that associate with the mouse mint protein and regulate notch signaling in nuclei, and to study the function and mechanism of mint - mediated transcription repression, yeast two - hybrid assay was used to screen proteins that interact with a fragment of mint ( f5, amino acids 2226 - 2959 ). from 4x106 yeast clones transformed with the bait plasmid and a cdna library of 9 dpc mouse embryo, fifty - one were positive for nutritional screening and p - galactosidase assay. restriction digestion identified 10 independent positive clones and these were analyzed by dna sequencing these clones represent 3 correctly fused cdna fragments, which are mint, alpha a - crystallin - binding protein i ( alphaa - crybpl ), and nuclear receptor co - repressor 1 ( n - corl ), respectively

    鑒于mwt基因編碼區較長，共有10799個堿基，故此我們將mint分為六段，分別命名為fi一f6 ，本研究以其中的f5 （ 222e959 ）和f6 （ 296s576 ）片段為研究對象，將h者分別插入pgb盯7載體中，結果顯示： mintfs可與核受體輔助抑制因子1階cori ） ， o晶狀體蛋白結合蛋白1hlphatcrybp入及mw加互作用，而f6可與igm的重鏈恆定區、泛素結合酶2l6和一個未知功能的新的蛋白基因進行結合。
  7. Bhattacharyya np, basu p, das m, et al negligible male gene flow across ethnic boundaries in india, revealed by analysis of y - chromosomal dna polymorphisms [ j ]. genome res. 1999 aug ; 9 ( 8 ) : 711 - 9

    李冬娜、應大君、區采瑩，等.中國海南島黎族人群y染色體上四個微衛星基因座的多態性研究.中華醫學遺傳學雜志[ j ] . 2003 ; 1 : 1 - 3
  8. Except the cortex 6 pair of aflp primers which might produce dna polymorphisms from the genomic dna of gastrodia elata bl. were filtered from 145 pair of e / m primers, 92 pair of e / p primers and 72 pair of e / p primers. all selected premiers were pairs of e / p, this did not coincided on the results in other plants, in which pair of e / m or p / m were used as aflp primers

    3通過對145對e m引物組合， 92對m p引物組合， 72對e p引物組合進行篩選，僅在e p引物組合中尋找出6組對天麻具多態性擴增且重復性好的引物，與其它植物中用e m或e p組合進行擴增不一致，說明採用aflp技術對天麻等中藥材進行研究，其引物組合與其它植物類材料研究相比有較大的差異。
  9. Two oligonucleotides were synthesized according to the sequence of p. furiosus extracellular a - amylase gene amya through the genbank and used as primers for pcr with p. juriosus genomic dna as the template

    根據genbank公布的p furiosus的-澱粉酶基因amya序列設計兩條引物，以p furiosus的基因組dna為模板進行pcr 。
  10. Clustering analysis showed that plants in the genus of p. salicina could be distinguished from those in the genus of p. ameniaca ; there was some genetic relationship among p. mume, p. salicina and p. ameniaca, of which p. ameniaca was closer to p. mume in genetic relationship ; and the distance between varieties of each genus was different, with the smallest being 0. 1138 and largest being 0. 7633. the genetic distance reflects genetic relationship between tested materials. the result that varieties of each genus were close to each other in genetic relationship testified the traditional morphology - based taxonomy from the genomic dna

    不同引物擴增出的帶型完全不同，聚類分析結果表明，李屬植物和杏屬植物能完全被區分開，李、杏和梅之間表現出一定的親緣關系，其中杏，梅之間的親緣關系較近，各屬品種之間都有不同的遺傳距離，最小距離為0 . 1138 ，最大距離為0 . 7633 ，遺傳距離的大小反映了材料間親緣關系的遠近，各屬內品種的親緣關系比較近，這一聚類結果從供試材料基因組dna分子水平驗證了傳統的形態學分類觀點。
  11. The results showed about 490bp dna fragments were amplified. because the amplified products is specific to the p - subclass of the proteobacteria, the amplification of the amoa gene may be a powerful molecular tools for detecting and analyzing ammonia - oxidizing communities in environment

    由於基於此引物的擴增對proteobacteria -亞科氨氧化細菌具有特異性，所以amoa基因片段的特異擴增為我們檢測和鑒定環境樣品中氨氧化細菌的種群提供了一個有效的工具。
  12. The e2 genes above of the prevalent strain ( guangxi yulin strain ) were cloned respectively into secreted expression vector ppic9k of eukaryotic expression system p. pastoris and transformed into p. pastoris by electroporation after linearization, 25 high - copied transformants were obtained by g418 screening. it was proved that the e2 genes were integrated stably into chromosome of p. pastoris by dot blot and dna sequencing

    豬瘟病毒e2基因的真核表達：分別將csfv兩個代表株的e2基因克隆入畢赤酵母（ p . pastoris ）分泌型表達載體ppic9k中，酶切線型化后電穿孔導入p . pastotis進行整合，經g418篩選得到25個高拷貝轉化子，經dna斑點試驗和dna測序證明外源基因e2穩定地整合到p . pastoris染色體中。
  13. The angiostatin baculovirus transfer vector was co - transfected with viral dna into sf9 cells according to the manufacturers protocol. to purify the recombinant virus, we used the plaque assay to screen the pure recombinant plaque and amplify it to generate p - 1 stock

    構建重組病毒：用已經構建好的angiostatin桿狀病毒轉移載體pbluebachiszb和病毒dna共同轉染sfg細胞，通過蝕斑實驗篩選出純的重組斑點並擴增產生p二病毒貯存液。
  14. The nucleotide sequences of its dna of the gxcr1 shared more than 95 % homology with 102 strains of p. spp

    該菌的its核苷酸序列與102株青黴菌的its核苷酸序列有95以上的同源性。
  15. Although the 16s rdna sequence similarity of isolate t7 with paenibacillus illinoisensis, paenibacillus pabuli and paenibacillus amylolyticus was 94 %, 95 % and 94 %, respectively, dna relatedness between isolate t7 and p. illinoisensis, p. pabuli and p. amylolyticus was 22. 32 %, 9. 14 % and 50. 62 %, respectively

    本文還從菌株g2和t1中，分別克隆了編碼p _ （ ）蛋白的glnb基因，用southern雜交的方法驗證了g2的glnb ，結果表明在g2中可能有2個拷貝的glnb基因。
  16. In order to get the soluble recombinant eo protein and inspect the protein expression status convinently, the egfp and eo gene were ligated into baculovirus transfer vector. with the co - transfecting sf9 cells of baculovirus recombinant transfer vector and linearized viral dna, and plaque purification in the posttransfection procedure, the pure recombinant baculovirus were harvested, which infected the sf9 cells for amplifying to generate a p - l stock. in the meantime, the fluorescence microscopy detection indicated expressed egfp protein to confirm the heterogenous protein expression of recombinant baculovirus. the pi - stock from a pure plaque was used to generate a high liter p - 2 stock, which was determined in liter as 1. 14 107pfu / ml by performing a plaque assay. when a volume of p - 2 stock infected the sf9 cells with moi 5 - 10 for expression, the strong fluorescence was obeserved on the day 3 of postinfection

    此外，為了得到可溶性重組eo蛋白並便於觀察重組蛋白的表達情況，我們將egfp基因與eo基因相連插入昆蟲桿狀病毒轉移載體中，與線性桿狀病毒dna共轉染sf9細胞后通過噬斑純化得到純的重組桿狀病毒，將其感染sf9細胞制備p1種子液，同時用熒光顯微鏡觀察綠色熒光蛋白的表達情況剔除表達效果差的重組桿狀病毒。再用p1種子液感染sf9細胞制備高效價的p2種子液。通過病毒液的梯度稀釋和噬斑測定，確定p2種子液的病毒滴度達1 . 14 10 ~ 7pfu ml 。
  17. In this research, the expression of dna polymerase p was examined in mnng treated cells, where untargeted mutation had been proved arisen

    Lp的表達：我們用western印跡方法檢測了經mnng處理后細胞中的p 。
  18. The method was modified by adding suitable insoluble pvp and higher concentration of p - mercapto ethanol in the extract solution. using the modified method, high quality genomic dna prepared from ginkgo, grape and myrica are colorless and are susceptible to digestion with the restriction endonucleases and can be used as template for pcr

    我們將ctab法稍做改良：研磨樣品時加入適量的不溶性pvp ，提高-巰基乙醇在提取液中的比例，從而提取到了無色、可酶解、可作pcr模板的高質量的銀杏基因組dna 。
  19. Mature embryo - derived calli of japonica rice ( oryza sativa l. ) cultivars nipponbare were transformed using agrobacterium tumefaciens strain agl1 carrying a binary vector pcas04 harboring the marker gene, neomycin phosphotransferase gene ( nptii ), driven by a promoter from the ubiquitin gene in maize, a promoterless p - glucuronidase gene near to the left border of t - dna for trapping gene and a strong promoter, rice actin - gb promoter, near to the right border of t - dna as activation tagging. in this system, co - cultivation was simplified, special selection stages and pre - regeneration stage were omitted, the whole process was almost under continuous light at 30 ? except co - cultivation and transgenic plants began to generate only after 7 weeks calli were induced

    在一步轉化系統中，光照高溫條件下培養的水稻愈傷組織從誘導開始經過4周時間就可以達到轉化實驗的要求，並且簡化、優化了整個共培養過程，省去了一篩、二篩和預分化步驟，只用7周的時間就可以初步得到再生轉化植株；共191塊愈傷組織得到125塊抗性愈傷組織，轉化頻率達到65 . 4 ，最後共得到99棵獨立來源的再生植株，抗性愈傷組織再生頻率達到79 . 2 。
  20. Using 20 out of 40 decamer random primers, random amplified polymorphic dna ( rapd ) was applied to analyze the diversity of 48 individuals of p. polyactis from five sampling areas in the yellow sea and the east china sea

    研究主要包括如下三個部分：一、小黃魚的遺傳多樣性分析了采自黃海和東海5個取樣海區共計4呂個小黃魚個體的隨機擴增dna多態性。
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