dna polymerase i 中文意思是什麼

dna polymerase i 解釋
dna聚合酶i
  • dna : 1. deoxyribonucleic acid 【生物化學】去[脫]氧核糖核酸。2. deoxypentose-nucleic acid 去[脫]氧戊糖核酸。
  • polymerase : n. 【生物化學】聚合酶。
  • i : pron （pl we ） 〈人稱代詞，第一人稱，單數，主格。 （poss adj my; obj me; poss pro mine ） 〉我。...
  1. Firstly, the major ampullate glands were got from araneus ventricousus. total rna and mrna of ampullate glands were isolated and purified. double strands cdnas were synthesized in the assistance of amv - rt and e. coli dna polymerase i etc. by reverse transcription and replacement synthesis

    首先剝離大腹園蛛主壺腹腺，提取總rna和分離純化mrna ，反轉錄合成cdna ，凝膠層析除去小於400bp片段，並在雙鏈cdna兩端引入ecor的銜接頭，對其進行磷酸化處理。
  2. In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein

    經酶切鑒定及dna序列測定，該基因為雞il - 2基因，其序列與sundick等報道的完全一致。在此基礎上，我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ （ tm ） ht中，構建重組表達質粒並進行確證性序列測定，重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ （ tm ） ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養， sds - page和westernblot分析顯示，表達的雞il - 2融合蛋白分子量約為18kda ，表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。
  3. The detection of ribozyme gene with two cleavage sites that cleaves plrv replicase gene were present in the second part of this paper. a conserved sequence of 35s promoter of plrv was found in gene pools. two primers were designed based on the conserved sequence and bamh i and ribozyme gene. the genomic dna of potato was amplified by the primers through polymerase chain reaction ( pcr )

    從許多資料中報道的plrv的35s啟動子的序列中找出一段保守序列，然後根據與核酶緊密相連的bamh和這段保守序列設計兩段引物，用這兩段引物通過pcr擴增轉基因馬鈴薯的基因組dna ，並進行檢測。
  4. In this research, the gpv hl isolate was propagated with 13 day ' s duck embryos. a pair of primers gflgr used to arnplify vp3 gene was designed using oligo4. i software according to the whole nucleotide sequence of gpv b isolate published by zadori. the major structural protein vp3 gene was amplified from the dna of gpv hl isoiate by polymerase chain reaction ( pcr ), and then cloned into pmdl8 - t vecter

    根據zadori等發表的gpvb株全基因核苷酸序列，藉助oligo4 . 1軟體設計了1對用以擴增主要結構蛋白vp3基因的引物gf / gr ，通過pcr技術，從病毒基因組dna中擴增出病毒主要結構蛋白vp3完整基因片段，經酶切鑒定后直接與pmd18 - t質粒載體連接。
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