dna shuffling 中文意思是什麼
dna shuffling
解釋
有性pcr-
The study results of this paper can serve the two scientific subjects of our teaching and research group as basic data calculation and elementary exploration. the two subjects are : constructing high activity - amylase genes by dna shuffling technology and studying on the evolution in vitro by mutational pcr ( with 5 - br - dutp as substitute partly ) and dna shuffling technology. - amylase ( ec 3. 2. 1. 1 ; 1, 4 - a - d - glucanohydrolase ) can catalyzes the hydrolysis of - 1, 4 - glycosidic bonds of starch from middle and liberates - maltose, - glucose and - limit dextrin stepwise
本試驗根據genbank已公布的黃單胞菌-澱粉酶基因的核苷酸序列由引物設計軟體premierprimer5 . 0輔助設計了一對引物（ primer & primer ） ，以pbluescript ks +和puc18 / puc19質粒為載體，用常規的pcr方法從xanthomonascampestrispv . malvacearum （ smith ） dye等七株黃單胞菌（ xanthomomasspp . ）的基因組dna中克隆得到8個基因片段，分別命名為zhyf001 zhyf008 。 -
It clearly allows us to engineer enzymes with novel functions and features by strategies, such as error - prone pcr, dna shuffling and stagger extension process ( step ), when very little is known about spatial configuration of proteins. therefore millions of years in nature can in principle be accomplished in the test within several years. according to the basic idea of directed evolution, we modified the a - aldc gene by error - prone pcr
它不需要事先了解酶的空間結構和催化機制，而通過在體外模擬自然進化機制（隨機突變，重組和自然選擇） ，利用易錯pcr ， dna改組，交錯延伸等方法，改造酶基因，定向選擇（或篩選）有價值的非天然酶，短期內在試管中完成在自然界需要幾百萬年的進化過程。 -
The display of penicillin g acylase from bacillus megaterium not only offers the possibility of applying this technology for the selection of penicillin acylases with new side - chain specificities, but also facilitates our screen of mutant library constructed by using dna shuffling technique
這是首次在噬菌體表面展示出有活力的巨大芽孢桿菌青霉素g酰化酶，為利用噬菌體展示技術進行青霉素g酰化酶突變庫的篩選奠定了基礎。 -
Therefore, a - amylase has been used widely in many industrial fields, such as glucose production, beer brewing, fermentation trade, textile industry, and so on. the study on a - amylase is one of the most active fields in enzyme industrial fields. with the development of biotechnology, more and more scientists and researchers attempt to use dna shuffling technology to breed, screen and even " create " new a - amylase genes with higher activity and other new characters
設計引物時，上下游引物5 』端分別添加了kpn和bamh酶切位點，克隆得到的基因片段和質粒載體都用kpn和bamh進行雙酶切后，進行酶連、轉化、篩選得到陽性重組子，經過藍白斑驗證、單酶切驗證、雙酶切驗證、 pcr驗證等一系列的驗證后進行測序。
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