dna t gene 中文意思是什麼

dna t gene 解釋
t 基因
  • dna : 1. deoxyribonucleic acid 【生物化學】去[脫]氧核糖核酸。2. deoxypentose-nucleic acid 去[脫]氧戊糖核酸。
  • t : 中世紀羅馬數字的160。
  • gene : n. 【生物學】基因。 dominant gene顯性基因。
  1. Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed

    本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游，構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，並進行了硬度和含糖量的分析。
  2. Two captive populations could n ' t be defined as separate evolutionary significant units ( esus ) because of lacking of genetic divergence between them, and should be considered as a single esu in the conservation of the species. by comparing the sequences of control region of mitochondrial dna from three species of crocodiles, it is revealed that the smallest genetic diversity exists between alligator sinensis and alligator mississipiensis. a portion of mitochondrial nd4 and cytochrome b gene of 3 species of crocodilian was sequenced

    百年來關于揚子鱷的分類地位存在著很多爭議，本文利用測得的揚子鱷（ alligatorsinensis ） 、暹羅鱷（ crocodlylussiamensis ）和灣鱷（ crocodylusporosus ）的mtdnand4和cytb基因序列，以及從genbank中獲得密西西比鱷、凱門鱷和海龜（ cheloniamydas ）的nd4基因和cytb基因相應片段，構建以海龜為外群的系統進化樹。
  3. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  4. As analyzed, ( 1 ) the rapd technique is highly sensitive to investigating genetic diversity in t. lepturus and e. muticus. t. lepturus exhibits lower polymorphism and genetic diversity than e. muticus ; ( 2 ) according to the analysis of the partial mitochondrial 16s rrna gene sequences, a very low intraspecific variation and considerably high divergence among species were found, which reveals a dual nature of conservatism and variability in mitochondrial 16s rrna gene ; ( 3 ) five primers generate the species - speeific rapd sites and these sites can be served as the molecular markers for species identification and ( 4 ) it can be proved at dna variation level that t. lepturus and e. muticus are of two species respectively pertainiag to different genera, which supported the nelson taxonomic conclusion

    分析結果表明: （ 1 ） rapd技術研究黃海帶魚和小帶魚的遺傳多樣性具有較高的靈敏度和檢出率，帶魚的多態比例和遺傳多態度均較小帶魚的低; （ 2 ）線粒體165出兇a基因序列在分析兩物種遺傳變異時表現出保守和變異的雙重特性，種內變異極小而種間較大: （ 3 ） 5個隨機引物擴增出種特異的ra衛d帶，可作為種間分子鑒定標記; （ 4 ）研究證實帶魚和小帶魚是不同屬的兩個種，從而在分子水平上支持了nelson分類系統的觀點。
  5. Four out of 150 random primers could detect dna polymorphism between the two mutants and the wild type arabidopsis thaliana, which provided a strong evidence for the truth of the mutants. only one primer gave a specific dna fragment about 1200bp which two mutants own but the parent do n ' t. we drew a preliminary conclusion that the fragment might be related to the salt - tolerance gene

    擬南芥抗鹽突變體的rapd分析結果表明， 150條引物中有四條引物在突變體和野生型擬南芥之間擴增出多態性，證明了dna水平突變的發生，其中1條引物在突變體的擴增產物比在野生型的擴增產物多出一個大小約為1200bp的片段，初步認為該片段可能與植物的抗鹽性有關。
  6. In this article, the author amplified dna sequences encoding the mature peptides of human intact and truncated igf - 1 gene from human genomic dna by the new way of alternative pcr established and named as exons - piecing together method by this laboratory. the igf - 1 and des ( l - 3 ) igf - l gene were cloned in pgem - t vector and then subcloned in expression plasmid pgex - 3x. two recombinant constructs known as pgex - 3x - igf - l and pgex - 3x - des ( l - 3 ) igf - l were transformed to e. coli dh5 a respectively

    我們利用外顯子拼接法從人的基因組dna中擴增了編碼完整型及截短型人igf - 1基因成熟肽的dna片段，並將其克隆至pgem - t載體中；經測序證明正確后，又構建了表達載體pgex - 3x - igf - 1及pgex - 3x - des （ 1 - 3 ） igf - 1 ，在大腸桿菌中進行了表達研究。
  7. Hovever, the gene encoding the mature papain peptide was amplified using pcr from genomic dna extracted mid - development carica papaya fruit. about 98. 8 % of cdna nucleotide sequence reported in literature were homologous to the responding sequences of our study. there are three introns in the gene, in which the content of a and t is 86. 0 %, 79. 5 % and 90. 6 % respectively

    同時本研究以木瓜基因組dna為模板，通過pcr反應獲得了編碼木瓜蛋白酶成熟多肽部分的核苷酸序列，序列分析表明該基因含有三個內含子，其長度分別為157bp 、 266bp 、 88bp ， at含量分別為86 . 0 ， 79 . 5 、 90 . 6 。
  8. In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein

    經酶切鑒定及dna序列測定，該基因為雞il - 2基因，其序列與sundick等報道的完全一致。在此基礎上，我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ （ tm ） ht中，構建重組表達質粒並進行確證性序列測定，重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ （ tm ） ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養， sds - page和westernblot分析顯示，表達的雞il - 2融合蛋白分子量約為18kda ，表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。
  9. Pcr analysis of resistant seedlings with nptii gene primers showed that 6 out of 12 seedlings detected had the 700bp fragment specific to the plasmid pig121, indicating that t - dna had been integrated into the genome of sweet cherry

    L ，負壓的適宜處理是lmmxio次。 6 、通過p r擴增，初步證明證明外源基回己轉入甜櫻桃。
  10. Using pcr technology, a 2. 4kb dna fragment, part of tryptopanase operon, containing a promoter, a regulator gene tnac located downstream of the promoter and a desired tryptopanase gene tnaa which can be expressed by the promoter from e. coli k - 12, was cloned to pmd18 - t vector. the dna sequence is the same as which was published on science

    為了證明質粒上的基因能表達出有活性的色氨酸酶，將這個dna片段克隆到pet28c質粒的bamhi和hind位點上，使該片段受t7rna聚合酶的啟動子控制，然後轉化噬菌體de3的溶源菌bl21 （ de3 ） 。
  11. Designing a specific primer pair based on the sequence of the gene ubia in e. coli mc4100, a dna fragment was amplified from the genomic dna of e. coli mc4100 by pcr and then was cloned into pucm - t vector. the cloned fragment was sequenced

    根據e . colimc4100的ubia基因全堿基順序，合理設計引物，以e . colimc4100基因組dna為模板進行pcr擴增，將pcr產物克隆于pucm - tvector后對其進行測序鑒定。
  12. On the bases of designing a primer pair, we obtained the coding domain sequence of rbp by pcr from cdna library. then the gene was cloned into pgem - t vector, the dna sequencing showed that the cloned gene was in agreement with the reported sequence. then the tageted gene of rbp was further subcloned into a procaryotic expression vector pbv220 and constructed recombinant plasmids was named pbv220 - rbp. in order to expresse rbp in procaryotic cell efficiently, the recombinant plasmids was introduced into e. colidhs a straints. expression of rbp was induced by temperature induction

    本實驗在合成該蛋白基因上下游引物的基礎上，利用pcr技術，從人睪丸cdna庫中釣取目的基因，並克隆于pgem - t載體中，經序列測定證明與文獻報道基本一致，再將目的基因從重組克隆質粒中亞克隆于pbv220原核表達載體中，轉化宿主菌，經溫度誘導后，進行sds - page電泳分析，發現在約21kd位置上出現了一條明顯的蛋白帶，與預期相符。
  13. First, the fact that the phenotypic variation in t1 mainly resulted from transformation of exogenous dna rather than ion beam mutagenesis was verified by rapd - pcr amplification to mutants, exogenous dna - transferred plants and ion beam - implanted plants. second, an absent band, marked by sieg - isso, was found in the rapd - pct amplification to t - 6 and its progenies, which meant that the corresponding mutation was hereditary. this mutation was located between exon 1791 - 2691 and exonl - 395 of abc - transporter gene

    T - 6和其t2代植株的rapd - pcr擴增結果中均存在缺失條帶s _ （ 168 - 1850 ， ）表明該缺失條帶對應的變異是可以遺傳的，該變異發生在abc鄭州大學博士論文摘要介即sporter基因exon1791一2691和exonl一395之間。
  14. T. identification of charactrization of transgenic mustard plants the putative transformant regeneration plants were assayed by pcr and pcr - southern blot analysis. both analysis the target bands were observed. so the integration of the cpti gene into mustard genome dna was confirmed. the result of insect - resistance showed that the transgenic plants are more resistant than non - transgenic plants

    轉基因芥菜植株的鑒定取再生苗的葉片提取dna ，進行pcr擴增和pcrsouthernblot分析，轉化再生植株大部分呈陽性，而非轉化的再生植株均為陰性，證明cpt基因已存在於芥菜基因組中。
  15. 2. an anther specific chimaeric male sterile gene expression box with a enhanced promoter ( ta29 ) driving coda gene was constructed and the expression box was inserted into binary vector p3301 that contains a l - phosphinothricin ( ppt ) - resistant selective marker gene and - glucuronidase ( gus ) reporter gene in t - dna region

    以增強的ta29啟動子驅動克隆的coda基因，構建成花藥特異性嵌合基因表達盒；將此表達盒插入雙元載體p3301 ，構建成以ppt抗性基因為選擇標記，以gus為報告基因的植物表達載體。
  16. Mature embryo - derived calli of japonica rice ( oryza sativa l. ) cultivars nipponbare were transformed using agrobacterium tumefaciens strain agl1 carrying a binary vector pcas04 harboring the marker gene, neomycin phosphotransferase gene ( nptii ), driven by a promoter from the ubiquitin gene in maize, a promoterless p - glucuronidase gene near to the left border of t - dna for trapping gene and a strong promoter, rice actin - gb promoter, near to the right border of t - dna as activation tagging. in this system, co - cultivation was simplified, special selection stages and pre - regeneration stage were omitted, the whole process was almost under continuous light at 30 ? except co - cultivation and transgenic plants began to generate only after 7 weeks calli were induced

    在一步轉化系統中，光照高溫條件下培養的水稻愈傷組織從誘導開始經過4周時間就可以達到轉化實驗的要求，並且簡化、優化了整個共培養過程，省去了一篩、二篩和預分化步驟，只用7周的時間就可以初步得到再生轉化植株；共191塊愈傷組織得到125塊抗性愈傷組織，轉化頻率達到65 . 4 ，最後共得到99棵獨立來源的再生植株，抗性愈傷組織再生頻率達到79 . 2 。
  17. In this research, the gpv hl isolate was propagated with 13 day ' s duck embryos. a pair of primers gflgr used to arnplify vp3 gene was designed using oligo4. i software according to the whole nucleotide sequence of gpv b isolate published by zadori. the major structural protein vp3 gene was amplified from the dna of gpv hl isoiate by polymerase chain reaction ( pcr ), and then cloned into pmdl8 - t vecter

    根據zadori等發表的gpvb株全基因核苷酸序列，藉助oligo4 . 1軟體設計了1對用以擴增主要結構蛋白vp3基因的引物gf / gr ，通過pcr技術，從病毒基因組dna中擴增出病毒主要結構蛋白vp3完整基因片段，經酶切鑒定后直接與pmd18 - t質粒載體連接。
  18. Momp gene is amplified by using pcr technologyfrom dna of l2 trachoma chlamydia. the pcr products are recombined with vectors of pmd18 - t. more over, the recombinant plasmids are colonged. the dna sequence analysis shows the insert fragment is momp dna

    本實驗採用pcr技術，從l _ 2型沙眼衣原體dna中擴增出momp基因，與pmd18 - t載體重組行t a克隆，並進行dna序列分析，證明所克隆基因為mompdna序列。
  19. And p4. 8 comparison were performed between the 3 t - cell vaccine and plasmid dna vaccine expressing hcv structural gene on therapeutic effects in hcv transplanting tumor murine model. the result showed that the former was obviously superior the latter, which indi

    將3種丁細胞疫苗與表達v結構基因的質粒a疫苗在v移他瘤小鼠捎型內進行治療對比，發現djj者明顯憂於後者，提示， t細胞疫祈在ncv感染的防治研究中可能具有較奸的發展汕景。
  20. The 1. 488kb dna fragment was sequenced and ligated to pbi121 vector and transformed into otsa deficient of e. coli strains ( ff4169 ). otsa gene is in charge of trehalose - 6 - phosphate synthesis in e. coli. in growth curve experiment, the transformants that carried the 1. 488kb dna fragment grew well in minimum medium, which contains 0. 5mol / l nacl, while control strains could n ' t endure it

    提取釀酒酵母的總dna ，以此為模板，採用pcr的方法從釀酒酵母中克隆出了1 . 488kb的海藻糖合成酶基因tps1片段，通過xba和sma雙酶切，與同樣經過xba和sma雙酶切的puc118載體質粒連接，轉入大腸桿菌dh5中，通過藍白斑篩選重組子。
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