dones 中文意思是什麼
dones
解釋
多恩斯-
Detection of antigen - binding affinity of mg7 recombinant phage antibody elisa was repeated to confirm the antigen - binding affinity of positive clones screened out in the former procedure ; these positive phages were examined by restriction analysis ( ecor i and hind iii ) ; competitive elisa was performed to test the inhibitory ratio of these positive clones to the binding affinity of mg7mab and its relevant antigen, the positive dones possessing apparent inhibitory effect were singled out for later use
X陽性克到駛知烘扳原kbbjg ）濁對陽性克隆進行限制隴臥賜析（ uwi和hedlll ）鑒定三用競爭elisatoljmg7重組噬菌體抗體性克隆對mg7單扶與其相應抗原結合忙的喇率，從中j ） ed出對mg7單抗與期眩抗原結合有抑余j效應的克隆用於進一涉研究。 -
Panning and enrichment ofmg7 recombinant phage antibody the transformed cells were infected with m13k07 helper phage to produce a phage form of mgy recombinant phage antibody library ; after three rounds panning of the library with the mgrbinding antigen highly expressed gastric cancer cell line kato iii, the mg7 scfv displayed phage dones were selected from the enriched recombinant phages by elisa
3 mg _ 7重組噬菌體抗體庫的淘篩用m13ko7輔助噬菌體感染轉化菌，以挽救出噬菌體形式的抗體庫；用高表達mg _ 7抗原的胃癌細胞系kato對抗體庫進行三輪淘篩；用elisa篩檢呈現有mg _ 7scfv的噬菌體單克隆。 -
5. expression and detection of soluble mg7 scfv the positive dones possessing apparent inhibitory effect were individually transfected into eco / / hb2151 ; after induction by iptg, perplasmic extracts was prepared from the induced transfectant ; the yields of soluble mg7 scfv produced by the transfected eco / / hb2151 were evaluated via immunodoting assay
7 u mg7scf64kilsrj將上述竟爭eu趴中篩蹦u的mo重組咱箔列莉b體陽性克隆感染hbzb2151大腸桿菌，經iptg誘導表達可溶11mg7schv ：牛憤iptgm菌的周質提取物。
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