double-bind 中文意思是什麼

double-bind 解釋
n. 名詞 1. 兩難，左右為難。
2. 窘境，困境。

  • double : adj 1 兩倍的，加倍的。2 雙的，二重的，雙重的；對，雙，兩，復。3 雙人用的；折疊式的。4 （意義）雙...
  • bind : vt （bound; bound 〈古語〉 bounden ）1 縛，捆，扎，綁；束；裹，卷 （about; around round）。2 （用...
  1. The mechanism is that the introduced complementary oligonucleotides can bind to the corresponding mrna or double - stranded dna in genome and form partial double - stranded molecules or triple - stranded nucleic acid molecules by sequence - specific and nonsequence - specific antisense action, thus the target gene will be orientationally blocked and expression of the target inhibited so that therapeutic effect could be attained. in this study, we designed a fragment of human c ii ta cdna in antisense orientation using mrna of c ii ta as template. the primers were designed based on 94 - 500 nucleotides segment in 5 " end of ciita gene so that the interested gene contained 407 base pairs which included two aug codons in 1 16 and 188 nucleotides as well as the splicing site between the first and the second exons

    本研究設計以c tamrna為模板的反義cdna片段，從c ta基因5 』端第94位到500位核苷酸段設計引物，目的片段407bp ，覆蓋第116和188位兩個aug密碼子，也包含了第一外顯子和第二外顯子間的剪接位點：用常規分子生物學方法構建了反義片段的腺病毒表達載體（ padeasy - 1系統） ；腺病毒載體經hek293細胞包裝產生含反義片段的重組腺病毒，用氯化銫密度梯度離心法獲得純化的高滴度腺病毒；進行體外基因轉移，分別用反義片段真核表達載體轉染p388d1細胞和用重組腺病毒感染hela細胞，觀察導入的c ta基因反義rna抑制細胞內組成型或誘導型c ta基因表達的作用，從而達到調控mhc -類分子表達的目的。
  2. Repeated experience. the double bind is a recurrent theme in the experience of the victim and as such cannot be constituted as a single traumatic experience

    重復經歷。雙重境地對受害人來說會反復出現，從而不構成單一創傷型經歷。
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