double-restriction 中文意思是什麼

double-restriction 解釋
雙重限制
  • double : adj 1 兩倍的，加倍的。2 雙的，二重的，雙重的；對，雙，兩，復。3 雙人用的；折疊式的。4 （意義）雙...
  • restriction : n. 1. 限制，限定。2. 拘束，束縛；自製。3. 【邏輯學】限定。
  1. A double - strand cdna fragment of dh ( diapause hormone ) gene was obtained from kt - pcr amplification. the fragment was digested by two restriction enzyme nde 1 / ecor 1 and then was joined with pet - 30a ( + ) plasmid which was digested by nde 1 / ecor 1 too. then the outcome was transformed into escherichia coli bl21

    Pcr產物經nde / ecor雙酶切后，與同樣雙酶切的pet - 30a （ + ）質粒連接並轉化至大腸桿菌（ escherichiacoli ） bl21中，經檢測篩選，成功得到陽性克隆。
  2. Linearized the expression vector ppic9k - p including the truncated mutant pokeweed antiviral protein ( pap ) gene by restriction endonuclease sal i and transformed it electrically into pichia pastoris gs115. mut + recombinants were selected by pcr and high yield mut + recombinant was picked out by double film immunoblotting

    本研究將含有n末端信號肽和c末端毒性區缺失的pap基因的表達載體ppic9k - p用sali酶切線性化后，通過電擊轉化整合p . pastorisgs115菌株細胞中。
  3. Pcr products were inserted into pbv - 220 with double digestion of restriction enduonuclease. the expression vectors of pbv - a pbv - b and pbv - c were constructed by orientaional cloning. through sds - page, bioactivity and function analysis of expressed protein, the function of phaa, phab and phac was confirmed

    菌株的亞克隆基因組片段中，分離出phaa 、 phab和phac三個基因片段，定向克隆至原核表達載體pbv220上，構建了三個原核生物表達載體pbv - a 、 pbv - b和pbv - c ，通過對表達載體誘導表達，表達蛋白產物的sds - page分析、生物活性與功能分析，確定了基因phaa 、 phab和phac的生物學功能。
  4. Phaa, phab and phac were inserted to pbv - 220 with double digest of restriction enduonuclease. the expression vectors of pbv - a, pbv - b and pbv - c were constructed by orientaional cloning. indefication of expression vector with restriction enduonuclease digest showed that phaa phab and phac were in right orfs

    將phaa 、 phab和phac片段雙酶切后，定向克隆至原核表達載體pbv220 ，構建了三個原核表達載體pbv - a 、 pbv - b和pbv - c ；經酶切分析表明，所克隆的三個基因phaa 、 phab和phac置於表達載體的正確閱讀框架下。
  5. The pcr products were purified and linked with pmd 18 - t vector respectively. the positive recombinants were selected and digested with double restriction enzymes respectively. goat fsh - a and p subunit genes which had sticky ends were purified and linked respectively with pf which also had the corresbonding sticky ends and were purified

    將所得的山羊fsh - 、亞基基因分別用加相應限制性內切酶酶切位點的引物大量擴增，回收后與pmd18 - t連接，篩選出陽性重組子，大量雙酶切回收獲得帶粘性末端的山羊fsh - 、亞基基因，將它們分別與同兩個酶大量雙酶切回收獲得的帶粘性末端的pf質粒進行連接，篩選陽性重組子。
  6. The recornbinant vector pcambia 1305. 1 containing s gene was confirmed by double digestion of restriction endonuclease

    經雙酶切鑒定證實s基因克隆到載體pcambia1305 . 1上。
  7. The recombinant vector pbi121 containing si gene of ibv was confirmed by pcr and double digestion of restriction endonuclease. agrobacterium fumefaciens eha105 with the recombinant vector pbi121 was obtained by tri - parental mating method

    用三親交配法將重組克隆導入根癌農桿菌，獲得了含pbi121重組質粒的eha105農桿菌菌株。
  8. Another approach of this thesis is to demonstrate that the lowest degree is 5 in order to make the b - spline patches hold the property of local adjustment if no specific 111 abstract restriction to the partition. most existed surfaces reconstruction methods adopt bezier tool and demand particular partition. the local scheme addressed in this thesis does n ' t restrict the partitions of fitted surfaces, and the fitting tools is biquartic b - spline surfaces with double interior knots and biquintic b - spline surface with single interior knots

    本文論述的「局部格式」調整方法不對剖分區域施加任何限制，主要採用內部具有二重節點的雙四次b樣條曲面和單節點的雙五次b樣條曲面做為擬合工具，給出了「局部」調整的方法，該方法能很好的保持拼接曲面的幾何特徵，克服了許多已有的重構方法僅採用簡單共線法處理幾何光滑性的弊端。
  9. Do not wait where there is a stopping restriction which prevents the driver from stopping to pick you up, for example where there are single or double yellow lines

    不要在不準上落客的禁區內候車，例如路邊劃有單黃或雙黃的地方。
  10. The writer has put forward a new train of thought that the power of arbitrary administrative execution will carry out well by using the double restriction and activation mechanisms. through the coordination of this double restriction and activation mechanisms, the best distrbution pattern of resources will apear, the value objective of morden administrative law will be accomplished

    本文正試圖從這一角度作以嘗試，筆者提出以制約與激勵雙重機制來確保行政強制執行權的良性運行，通過制約機制與激勵機制的協調運作，形成最佳的資源配置格局，實現現代行政法的價值目標。
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