e antibody 中文意思是什麼

e antibody 解釋
e抗體
  • e : （pl E s e s ）1 英文字母表第五字母。2 【音樂】E調，E音。3 E字形。4 〈美國〉（順序）第五等，（成...
  • antibody : n. 【醫學】抗體。
  1. Expression of human fab antibody against keratin in e. coli and its renaturation

    抗體在大腸桿菌中的表達與復性研究
  2. The sucking mouse brain were inoculated with mdj - 01 strain to make electron microscopic examination, results showed that the virus was a spheral particle with membran which had a diameter of about 40 nm. by indirect fluorescent antibody test mdj - 01 strain was identified with tbev. a part of region encoding e protein was expanded by rt - pcr and sequenced. the nucleotide sequences of two strain viruses were compared with sequences in genbankjsequence homology analyses revealed mdj - 01 strain and senzhang strain had the highest homology with tbev oshima5 - 10, respectively, which were 95 %, 94 %. mdj - 01 strain was identified with tbev again

    應用間接免疫熒光試驗進行血清學鑒定，結果表明mdj - 01株為tbev 。通過rt - pcr技術擴增部分e蛋白序列並測序，在genbank上進行同源性比較，發現mdj - 01株和森張株與tbevoshima5 - 10株的同源性最高，分別為94 、 95 ，從分子生物學水平上進一步證明mdj - 01株病毒為tbev 。在鑒定的基礎上，本實驗對兩株病毒進行了核苷酸全序列測定。
  3. Only antibody of e of second liver virus and core antibody two positive, transaminase is normal also, this does not have the requirement of second liver

    僅乙肝病毒e抗體和核心抗體兩項陽性，轉氨酶也正常，這不具備乙肝的條件。
  4. Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2. construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr. the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e. co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )

    Mg _ 7重組噬菌體抗體庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取並分離mrna ，反轉錄成cdna ；利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區基因，並通過? dna連接子將二者連接起來形成mg _ 7單鏈抗體基因；將mg _ 7單鏈抗體基因插入pcantab5e ；將連接產物轉化感受態tg1大腸桿菌，制備細菌形式的mg _ 7重組噬菌體抗體庫；通過菌落計數和限制性酶切分析（ ecor和hind ）評估mg _ 7重組噬菌體抗體庫的容量和重組率。
  5. Ability to a - complement of the ea / ed protein was determined by the addition of onpg western blot test with rabbit to e. coli p - galactosidase pcab as first antibody was used to verify the fusion proteins

    以兔抗p半乳糖昔酶抗體做western blot以證實與gst融合表達的ea工d蛋白是p半乳糖昔酶的成分。實驗結果1
  6. The extracellular 100pl ( ecl1 ) and ecl2 was linked by disulfide bond to maintenanced the stability of the protein secondary structure. in recent years, we showed that ccr5 as a co - receptor could interact with hiv - 1 infect ion. ccr5 was paid closed attention to since it was cloned in 1996. the aim is to - obtain the sequence of first extracillular domain of 3 chemokine receptor 5 ( ccr5 ) n - terminal gene fragment with high level expression in e. coli and to prepare its specific antibody f ( ab ' ) ;, and its detected method

    Ccr5具有g蛋白偶聯受體家族（ g - proteincoupledreceptors ， gpcrs ）所特有的7個跨膜區（ transmembrinedomains ， tm ） ，呈螺旋， tm的氨基酸有很高的保守性，膜外第一襻（ extracellularloop1 ， ecl1 ）和ecl2之間有二硫鍵相連，以維持蛋白質二級結構的穩定性。
  7. Okamoto h, tsuda f, akohane y, et al. hepatitis b virus with mutation in the e antigen - negative phenotype in carriers with e antibody to e antigen [ j ]. j virol, 1994, 68 : 8102

    王小飛，劉華瑞，王錦蓉，等.乙型肝炎和肝癌患者乙型肝炎病毒前c區1896位點突變的研究[ j ] .中華傳染病雜志， 1996 ， 14 : 11
  8. 2. to establish a neutrajizing - inhabition time - resolved fluoroimmunoassay ( trfia ) of hepatitis b virus e antibody ( hbeab ) and hepatitis b virus core antibody ( hbcab ) based on the competion. the assay ranges of standard cures were 0 - 16ncu / mland 0 - 8ncu / ml. the within - run coefficient variations for standard samples were less than 10 %. compared this method with radioimmunoassay, the sensitivities were 92. 8 % and 93. 3 %

    以中和抑製法建立了乙型肝炎病毒e抗體時間分辨熒光免疫分析法（ hbeab - trfia ）和乙型肝炎病毒核心抗體時間分辨熒光免疫分析法（ hbcab - trfia ） ，標準曲線分析范圍分別為0 - 16ncu ml和0 - 8ncu ml 。
  9. With the base of related research on nucleic acid immunization, the technology was used to develop th e research and application of cpti transgenic plant. a new antibody preparation method by nucleic acid immunization to assay the expression of the transgenic gene was explored and compared with the traditional one which immune animal by protein

    在國內外大量核酸免疫的研究基礎上，本研究首次將核酸免疫技術應用於轉基因植物檢測研究中，探討一種核酸免疫法制備抗cpti抗體來檢測基因表達的方法，並與傳統的蛋白免疫方式制備抗體進行比較。
  10. In this prospectie, open - label, randomized, controlled trial, we enrolled 21 high immunological risk patients ( i. e., panel reactie antibody > 20 % or preious transplant )

    在此前瞻性開放隨機對照試驗中，我們納入了21例免疫高危患者（例如，此群體反應性抗體> 20 %或者是再次移植患者）
  11. In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein

    經酶切鑒定及dna序列測定，該基因為雞il - 2基因，其序列與sundick等報道的完全一致。在此基礎上，我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ （ tm ） ht中，構建重組表達質粒並進行確證性序列測定，重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ （ tm ） ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養， sds - page和westernblot分析顯示，表達的雞il - 2融合蛋白分子量約為18kda ，表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。
  12. Ptxb1 - hng and ptxb1 - m - insulin are expressed in e. coli successfully. after sds - page and densitometric scan analysis, the results show that the expression level of hng fusion protein is above 40 % and m - insulin fusion protein above 50 % of total bacterial proteins. western - blot result demonstrated m - insulin fusion protein had specific reaction with mouse anti human insulin antibody ( igg )

    Pbv220 ? hng在大腸桿菌中未檢測到表達，后兩個克隆在大腸桿菌bl21 （ de3 ）中獲得高效表達， hng及m - insulin融合蛋白表達量分別佔全菌蛋白的40及50左右；經western - blot鑒定m - insulin融合蛋白可以與小鼠抗人胰島素單克隆抗體（ igg ）發生抗原抗體結合反應。
  13. Anti - p21 mouse monoclonal antibody from beijing zhongshan biotechnology anti - mouse or anti - rabbit igg secondary antibody from santa cruz biotechnology ly294002 from sigma biotechnology tritonx - 100 from boehringer mannhein gmbh fluorescein isothiocyanate ( fitc ) conjugated anti - mouse igg antibody was purchased from beijing zhongshan biotechnology hepes from e. metck darmstadt methods superovulation and collection of eggs for superovulation, female kunming mice 4 - 5 week old were injected with pregnant mare serum gonadotropin ( pmsg ), and after 46 - 48 hours with human chorionic ginadotropin. ( hcg ). one - cell fertilized eggs were collected on the next day from oviduct of females

    取4一5周齡成熟雌性昆明系小白鼠，腹腔注射pmsg （孕馬血清促性腺激素） 10iu ， 46一48小時后腹腔注射hcg （人絨毛膜促性腺激素） 1oiu ，將注射hcg后的雌鼠與8周以上的成熟雄鼠合籠交配，次日檢察陰栓，將查到陰栓的雌鼠處死，取輸卵管于mz培養液中，解剖鏡下撕開壺腹，釋放細胞團，然後用300林歲nil透明質酸酶消化去除顆粒細胞，口控吸管將卵細胞在m :中反復清洗，然後置於孵箱中，根據時間點收集g2期細胞。
  14. A dot - ppa - elisa has been developed to detect the specific antibody against streptococcus suis. the antigen was isolated from group c d e r and type 2 streptococcus suis by three different method : ( a ) autoclave extraction method, ( b ) hcl - extraction method, and ( c ) fuller ' s method

    本研究選取c 、 d 、 e 、 r群及2型鏈球菌標準菌株，以高壓法提取抗原建立了檢測豬鏈球菌病血清抗體的dot - ppa - elisa方法。
  15. Scfv antibody library was constructed after transform of pcantab5e into e. coli tgi

    經輔助噬菌體m13k07超感染，將scfv展示于噬菌體表面。
  16. So in this paper, effects of dietary protein levels on the content of protein and amino acid profile in hepatopancreas and ovaries during ovarian maturation are investigated on basis of analyzing protein content and amino acid composition and content in hepatopancreas and ovary of broodstock in nature. influence of dietary protein on concentration of lipovitellin in ovary of broodstock after purification and preparation antibody of lipovitellin from mature ovaries of e. sinensis is evaluated. and nutritional requirement for arginine and lysine, and interaction between protein and vitamin b6 of juvenile e. sinensis are also studied

    華東師范大學2003屆博卜學位論文為此，本研究在弄清中華絨鰲蟹自然條件下卵巢發育過程中肝胰腺和卵巢的蛋白質含量及其氨基酸組成的基礎上，探討了餌料蛋白質水平對其卵巢發育過程中肝胰腺和卵巢的蛋白質含量及氨基酸組成的影響;在制備中華絨鰲蟹成熟卵巢卵黃磷蛋白抗體的前提下，研究了蛋白質營養對成熟期卵巢卵黃磷蛋白合成的影響:並對仔蟹期的部分必需氨基酸適宜需要量，蛋白質和維生素b6的相互作用進行了研究。
  17. ( 4 ) in artificial pepsin digestive system with ph2. 0, due to the acid denaturation, the pigg was hydrolyzed wholly, in the system with ph3. 0 and 4. 0, although not all entirely pigg could be detected, but the pigg antibody activities against e. coli still existed without changing, this indicates that the f ( ab " ) 2 with antigen - binding activity still remain complete during digesting process

    （ 4 ）在ph2 . 0的人工胃液消化體系中，由於酸變性pigg全部水解；在ph3 . 0和ph4 . 0的人工胃液消化體系中，盡管檢測不到所有的pigg ，但pigg仍可與e . coli特異性結合且凝集價未發生變化，表明在消化過程中具有抗原結合活性的f （ ab ） _ 2片段仍可完整地保留下來。
  18. The high titer specific ndrg2 antibody is indispensable to reseach deeply the functions or the tissue and subcellular distribution features of ndrg2, ha order to prepare ndrg2 antibody, the whole ndrgl sequence was cloned into prset - a vector and two truncated sequences of ndrg2 were cloned into pgex - 4t - l vector. after induced by iptg, the fusion proteins were expressed in e. coli ; rabbits immunized with the whole length ndrg2 protein were reinforced with two shortened fragments of ndrg2 ; after immunization, rabbits produced high titer antiserum against ndrg2. then antisemm was absorbed using ndrg2 antigen immobilized on nc filters, the purified product of antiserum shows high special to ndrg2 protein, and the separated inclusion body of 6his - ndrg2 will be useful for the further reseach

    為制備高效價的ndrgz抗體，分別構建了prset a雌、 pgex4t d唾倉和pgex4tl七三種原核重組表達質粒，並在大腸桿菌中誘導表達出相應的融合蛋白；用全長gstjqdrgz蛋白免疫兔，然後用gst ndrgz人和gstjqdrgze片段加強免疫，經免疫得到了較高效價的兔抗人ndrz多克隆抗血清，利用固定於硝酸纖維素膜上的ndrgz抗原親和吸附純化抗血清，提高了ndrgz抗體的特異性；並對包涵體形式表達的6his ndrgz進行初步的分離純化。
  19. The recombinant pil - 10 can be a valuable therapeutic agent for the diseases with overproduction of inflammatory cytokines. we also constructed the recombinant e. coli strains that highly express pil - 12 two subunits with immunological activity. they can be used to generate monoclonal antibody against pil - 12

    構建的高效表達pil - 12的兩個亞基的基因工程菌株，能產生具有免疫原性的蛋白，可用於單克隆抗體的生產，為pil - 12在臨床上的應用奠定基礎。
  20. Kurane s, kranss jc, watari e, et al. targeted gene transfer for adenocarcinoma using a combination of tumor - specific antibody and tissue - specific promoter [ j ]. jpn j cancer res, 1998, 89 ( 11 ) : 1212

    李芳，伍新堯，郭語彬，等.陽離子脂質體介導肺癌細胞基因轉移效率的比較研究[ j ] .中山醫科大學學報， 2000 ， 21 （ 6 ） : 484
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