early promoter 中文意思是什麼
early promoter
解釋
早期啟動子-
Ie protein have multiple functions, such as conversion of the host cell into a metabolically activate states, activation of viral early genes in the early phase, repression of their own transcription. ie 180 proteins can stimulate transcription from both homologous such as the icp4 of hsv ( herpes simplex virus ) and heterologous viral and cellular promoter such as - globe protein
經分析偽狂犬病病毒立即早期蛋白( immediate - earlyprotein180 , ie180 )的蛋白質結構,發現ie180具有轉錄激活因子的結構特徵,能與類rna聚合酶結合, ie180基因缺失突變體對sv40及cmv啟動子調控作用的研究,是考察ie180能否作為轉錄激活因子應用的關鍵。 -
The ie 180 mutant combined with two special protein binding - sites and inhibited the promoter transcription. accidentally in the approach showed that the plasimd pcdna3 show as activator to sv40 and cmv early promoter. the result is acquired by instantaneous transinfect
這說明不同的ie180突變體,對于sv40啟動于這類在轉錄起始位置一側只有一個「 5 』 atcgt 3 』 」特徵蛋白質結合序列的11類基因啟動于,可分別表現出抑制活性與激活性。 -
It is show the mutant consists of a nuclei binding site and dna - binding domain is enough to negative regulation. a report plasmid pcat that we constructed has a cat gene expressed by a cmv early promoter
( 465 1079氨基酸段)對sv40啟動子明顯的抑制活性,因為它只包含dna結合域和核結合位點,說明發揮轉錄抑制功能的關鍵是ie180的dna結合域。 -
And then a 378bp acci fragment in pbtk2. 6 was replaced by a fragment containing the immediate early promoter of cytomegalovirus and bovine growth hormone polyadenylation signal derived from pcr3 - uni, a eukaryotic expression plasmid
在此基礎上,進一步用tthlll酶切后,補平插入帶有sv40啟動子的大腸桿菌?半乳糖苷酶報一告基因( lacz ) ,獲得通用轉移載體pltk - uni 。
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