egfp 中文意思是什麼

egfp 解釋
將綠色熒光蛋白
  1. First, to construct a recombinant plasmid pegfp - c - fos with c - fos promoter and egfp, and then transfect it into human bladder transitional cell carcinoma biu - 87 cell ; second, based on the changes of the expression of gfp in the biu - 87 cell which induced by the aconitine and hab toxins, the concentration of the hab toxins could be detected

    目的：構建一個含c - fos啟動子和egfp報告基因的pegfp - c - fos重組質粒載體。體外轉染膀胱癌biu - 87細胞后，利用赤潮毒素作用后細胞表達綠色熒光蛋白的變化來檢測赤潮毒素，初步建立一種以細胞為基礎受體水平的赤潮毒素檢測方法。
  2. Plaque experiment indicated that hasnpvgp64 + egfp + can produce infectious virions in sf21 cells

    Hasnpvgp64 + egfp +感染sf21細胞的空斑實驗，發現在sf21細胞中形成有感染性的病毒粒子。
  3. Construction of recombinant plasmid pires2 - egfp cck and its expression in hamster

    質粒的構建及其在倉鼠體內表達
  4. Construction of p21 egfp fusion gene expression vector and its expression in hrpe cells

    15細胞乙型肝炎病毒復制的實驗研究
  5. Cationic polymer mediated plasmid encoding egfp retrograde transfection in retinal ganglion cells

    基因逆行轉染視網膜神經節細胞
  6. Construction of the eukaryotic expression vector pires2 - egfp - axin and its expression in glioma cells

    的構建及其在神經膠質瘤細胞內的表達
  7. Experimental study on transferring egfp gene into the retina of rat mediated by microbubbles

    2腫瘤的三維彩色多普勒超聲成像與病理對照研究
  8. It was confirmed by restriction enzyme digestion analysis that egfp fusion expression plasmids of scfvs were successfully constructed

    的序列正確，經酶切鑒定證實成功地構建了綠色熒光蛋白基因融合表達載體。
  9. To follow the differentiation of lepcs in vivo, lepcs were tagged with reported gene egfp, dsred and lac z by retroviral methods

    採用逆轉錄病毒介導的基因轉染手段，將lepcs標記lacz 、 egf戶和osreo作為報告基因。
  10. Furtherly expression phase analysis showed that orf2 appeared in different form during infection. to reveal the function of haorf2, vha - orf2 - gfp recombinant virus which tagged with egfp - orf2 fusion protein was generated

    構建了ha2與綠色熒光蛋白融合表達的重組病毒vha - orf2 - gfp ，對該重組病毒感染細胞后，融合篡碩士學位論文mast卜r 』 s 』 r拼ifs
  11. 5. mscs are excellent seed cells for treating nervous system diseases, damages, genetic defects and degeneration diseases. here, human mscs with enhanced green fluorescent protein ( egfp ) were injected into the stri

    87士又刀6咖in ，差異顯著o 0刀5人表明植入的骨髓sc在腦內微環境的作用下能夠在一定程度上發揮作用，有望成為治療pd的種子細胞。
  12. According to the gene sequence and secondary structure of hcv ns5b, we design the sirnas targeting ns5b gene following with the requirement for sirnas design from tuschl et. al and synthesize it from dharmacon company ; hepg2 cell stably expressing ns5b - egfp protein was trasfected by synthesized sirnas with electroportion, the non - transfected cell and non - specific sirnas transfected cell are c onsidered as control group ; inhibitory effect of sirnas was investigated by fluorescence microscope with dapi dyeing and by semi - quantitative rt - pcr

    然後根據dsrna設計原則，結合nssb基因的序列特徵，藉助生物信息學軟體設計了針對nssb基因的sirnas ，並交由公司化學合成；電穿孔法轉染上述穩定轉染的細胞克隆，同時分別以非特異的sirnas轉染組和空白轉染組為對照， dapi染色后通過熒光顯微鏡和內標化rtpcr檢測，初步證實了化學合成的sirnas可以特異阻斷nssb基因的表達。
  13. To study the localization of the peptide, we also generated recombinant viruses containing the ctl - egfp fusion gene, following observation of infected larvae by such viruses will provide insight into the function of baculoviral conotoxinlike genes

    為了研究毒素在蟲體中的定位，構建了類蝸牛毒素和增強的綠熒光蛋白融合的重組病毒racbacctlgfp2和rhabacctlgfp ，為下一步研究奠定了基礎。
  14. In order to get the soluble recombinant eo protein and inspect the protein expression status convinently, the egfp and eo gene were ligated into baculovirus transfer vector. with the co - transfecting sf9 cells of baculovirus recombinant transfer vector and linearized viral dna, and plaque purification in the posttransfection procedure, the pure recombinant baculovirus were harvested, which infected the sf9 cells for amplifying to generate a p - l stock. in the meantime, the fluorescence microscopy detection indicated expressed egfp protein to confirm the heterogenous protein expression of recombinant baculovirus. the pi - stock from a pure plaque was used to generate a high liter p - 2 stock, which was determined in liter as 1. 14 107pfu / ml by performing a plaque assay. when a volume of p - 2 stock infected the sf9 cells with moi 5 - 10 for expression, the strong fluorescence was obeserved on the day 3 of postinfection

    此外，為了得到可溶性重組eo蛋白並便於觀察重組蛋白的表達情況，我們將egfp基因與eo基因相連插入昆蟲桿狀病毒轉移載體中，與線性桿狀病毒dna共轉染sf9細胞后通過噬斑純化得到純的重組桿狀病毒，將其感染sf9細胞制備p1種子液，同時用熒光顯微鏡觀察綠色熒光蛋白的表達情況剔除表達效果差的重組桿狀病毒。再用p1種子液感染sf9細胞制備高效價的p2種子液。通過病毒液的梯度稀釋和噬斑測定，確定p2種子液的病毒滴度達1 . 14 10 ~ 7pfu ml 。
  15. In order to form a chloroplast transformation system of d. salina, we have conducted some studies including its sensitivity to antibiotics, the activity of promoter, cloning of the chloroplast genes and construction of transformation vectors, so far a pilot transformation system of the d. salina chloroplast has been completed. methods : the sensitivity of d. salina to seven antibiotics or herbicide used commonly in gene engineering was studied and the biological activity of atpa promoter from c. reinhardtii chloroplast was tested by using enhanced green fluorescent protein ( egfp ) as a reporter. primers were designed in the conservative encoding regions according to the chloroplast genomes from four algae which have close genetic relationship with d. salina, and the sequences of 16s rrna, chll and chln of d. salina chloroplast were cloned and sequenced, respectively

    方法:根據杜氏鹽藻的近緣藻類的葉綠體基因組序列資料，在基因編碼區的高度保守區域設計引物，克隆了杜氏鹽藻葉綠體165識na基因、咖l基因和ch n基因，並分別以165識na基因和chln基因序列為同源片段，以cat和bar基因為篩選標記基因構建了三套杜氏鹽藻葉綠體轉化載體: 2鄭州大學2003年博士學位論文杜氏鹽藻（ d ~ iiellasalina ）葉綠體轉化研究pds165一eaf 、 ptn1269一bar和psp72一5一bar一3 ，用基因槍法轉化杜氏鹽藻，初步建立起杜氏鹽藻葉綠體轉化體系。
  16. Furthermore, the egfp - eo gene was also cloned into pci - dhfr, a cho ( dhfr " ) cell expression vector. following the transfection and mtx selection, some cho cells presented green fluoresence, which will establish a good foundation for production of soluble eo glycoprotein and investigating its biological functions

    將p2種子液以moi5 - 10接種指數期sf9細胞，三天後呈現出最強的熒光。我們還將egfp - eo融合基因插入哺乳動物細胞表達載體pci - dhfr中，構建重組質粒pci - dhfr - egfpeo 。
  17. This study we acquired the coding region of hcv ns5b gene by pcr of hcv full length genome and construct the recombinant plasmid pegep n3 - ns5b ; with the different concentration of g418 in the culture medium, we think the selection concentration of g418 for hepg2 cell is 800 g / ml ; the recombinant plasmid was transfected into hepg2 cell by lipofectamine2000 cells containing stable transformants were selected by the ability of resistance to g418 and isolated with the limited dilution. the stably transfected cell line expressing ns5b - egfp fusion protein was obtained by the detection under fluorescence microscope and rt - pcr

    本研究首先從hcv基因組中擴增出nssb基因，構建了nssb基因與報告分子egfp （增強型綠色熒光蛋白）基因的融合基因真核表達質粒pegfpn30 ；通過含有不同g418濃度梯度的培養液培養hepgz細胞，確定了篩選用g4181作濃度為800pg ml ；利用脂質體法將該重組質粒轉染hepgz細胞，經過有限稀釋法和g4壓力選擇，應用熒光顯微鏡和rtpcr檢測，獲得可穩定表達nssbegfp融合蛋白的hepgz細胞克隆。
  18. Infection assay in vitro showed that overexpression of p78 / 83 had n ' t any evident effect on the virus growth and viral assemble. it is notable that the fusion protein can be assembled to virions. via actin dissemination in vac - orf9 - gfp infected cells, p78 / 83 - egfp might colocalize with actin

    對vac - orf9 - gfp感染sf21細胞作電鏡時相切片分析，結果表明，表達p78 83 - egfp融合蛋白的重組病毒，對病毒粒子的形態發生沒有明顯可見的影響。
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