electrophoresis of protein 中文意思是什麼

electrophoresis of protein 解釋
蛋白電泳
  1. Protein gel electrophoresis identification of new wheat varieties

    小麥谷蛋白亞基
  2. In this paper, the genetic diversity of qinghai - lake naked carps was studied for the first time by methods of chromosome karyotype, protein ( isomzy ) electrophoresis and mtdna rflp. the results of chromosome karyotype indicated that : ? the diploid chromosome number of qinghai - lake nakked carp was 2n = 92 that comprised of 18 metacentric chromosomes, 18 submetacentric chromosomes, 16 telocentric chromosomes and 40 acrocentric chromosomes, and the karyotype formula was 18nvh8snvh6st + 40t, nf = 128

    染色體核型研究結果表明；青海湖裸鯉染色體總數2n = 92條，由18條中著絲粒染色體、 18條近中著絲粒染色體、 16條近端著絲粒染色體和40條端著絲粒染色體組成，其核型式為： 18m + 18sm + 16st + 40t ， nf = 128 。
  3. Get the inclusion bodies 2 ) western blot analysis of fusion protein expression ( 1 ) electrophoresis ( 2 ) transfer proteins from gel to membrane ( 3 ) blocking ( 4 ) incubation with primary antibody ( 5 ) enzyme conjugate incubation ( 6 ) substrate incubation. 3 ) gst detection module with cdnb enzymatic assay 3 purification of gst fusion proteins 1 the denaturalization of inclusion bodies. 2 purification using glutathione sepharose 4b column wash matrix with 1 pbs, prepare a 50 % slurry for batch purification method, pack column with matrix slurry

    三、 gst一hbrp重組蛋白的純化1 .超聲破碎細胞，離心，上清和沉澱進行sds一page電泳分析2 .樣品處理提取包涵體，變性后，加人用pbs平衡過的以utal腸onesepb抓脫4b ，室溫下孵育3 .以utadtionesepharose4b柱純化以utad雲onesepharose4b柱的準備根據毛山lel ，決定純化所需的gll衛ta1如oneseph娜se4b的柱床體積，用預冷的1xpbs清洗cldtathionese戶， se4b ，得到50 %的基質
  4. ? as same as most other schizothoracinae fishes, qinghai - lake nakked car ps may be tetraploid that already were diploidization ; ? qinghai - lake nakked carps arose from some primitive barbinae fishes, which gradually became special population adapting to high altitude environment by involvement and natural selection, on protein electrophoresis, out of 20 protein loci among 132 individuals in the present experiment, only t mdh > amy est and pod showed polymorphism

    在蛋白水平上，共檢測了132尾裸鯉的20個遺傳座位，僅發現tf ， mdh 、 amy 、 est和pod五個座位表現出多態性，其中， tf 、 mdh 、 amy和est四個座位均有一對等位基因；而pod座位表現出復雜的電泳圖譜，未進行深入分析。
  5. Purification and characterization of phytase from a. niger an 01001 a. niger an01001 was inoculated on solid media and cultivated at 30 for 5 days. proteins were extracted from solid - state fermentation with 50mm acetate buffer ( ph5. 5 ). the molecule weight of the phytase protein was determined as about 78kd by sds - page. the purification procedures include ammonium sulfate precipitation, deae - cellulose ion - exchange chromatography, gel electrophoresis and electroelution

    3 .植酸酶的分離純化及其性質研究黑麴黴ano1001經固體發酵，用緩沖液抽提后，經硫酸按沉澱， deae一纖維素離子交換層析，聚丙烯酞胺凝膠電泳和電洗脫等純化步驟獲得的植酸酶，用sds一page檢測為一條均一譜帶，其分子量約為78kd 。
  6. The two - dimensional electrophoresis of protein from pollen wall between citrus grandis var. shatinyu hort. and citrus grandis

    沙田柚和酸柚花粉壁蛋白的雙向電泳分析
  7. In recent measuring method, protein electrophoresis is rapid, accurate, steady and economic, not affected by environment condition, we improve reactive system of our national tentative com protein gelatinous electrophoresis, and that which is more adapted to imaging process

    在玉米種子純度檢測的方法中，蛋白質凝膠電泳法具有快速、準確、穩定、經濟、不受環境條件影響等特點，因此，選取此方法作為種子純度檢測方法。對國家試行的玉米蛋白凝膠電泳法的反映體系進行了改進，使得到的圖像更清晰。
  8. The results from sds - page presented that there were three female specific protein subunits with molecular weights of 123 kd, 120 kd and 91 kd, respectively. we can conclude the higher molecular compose of two subunits ; the results from two dimension electrophoresis showed the isoelectric points of two female - specific spots with molecular weight of about 120kd were 5. 5 and 5. 7. immunodiffusion reactions demonstrated that vg existed both in female fat body and hemolymph, which as vn was deposited in the ovary, while not in the male

    Page電泳結果表明：麗蠅蛹集金小蜂明顯存在2條雌特異性帶-卵黃蛋白，分子量分別為181kd和136kd ； sds - page電泳分析：存在3條雌特異性帶，其分子量為123kd 、 120kd和91kd ，由此，可推定卵黃原蛋白（ vitellogenin ， vg ）和卵黃磷蛋白（ vitellin ， vn ）由2個蛋白組成，其中分子量較大的蛋白由2個亞基組成；雙向電泳結果顯示，在120kd附近有兩個特異性點，其等電點為5 . 5和5 . 7 ；雙擴散表明，麗蠅蛹集金小蜂卵黃磷蛋白的抗血清與雌隱成蟲蟲體、脂肪體、血淋巴和卵巢勻漿液均有免疫沉澱反應，而與雄蜂血淋巴無免疫反應，說明了vg與vn具有免疫同源性，是雌特異性蛋白，且由脂肪體合成。
  9. In the second part, polyphasic taxonomy methods such as morphological method, physiological and biochemical method, dna g + cmol %, soluble protein electrophoresis and 18s rdna sequence analysis were used for systematics of all strains isolated and purified with the method above

    本論文第二部分採用形態學、生理生化特徵、 dnag + cmol 、可溶性蛋白電泳及18srdna序列分析等分類技術對所分離的16株甲真菌進行了系統的分類研究，從而初步確定了16株甲真菌的分類地位。
  10. At last, we got the pure protein with the determination of electrophoresis. the molecular weight of the enzyme is estimated at 30903 through gel filtration and sds - page electrophoresis. the isoelectric point is 8. 6 ?. 3

    經sds凝膠電泳和凝膠過濾層析兩種方法測定該酶的分子量分別為33113dalton和28840dalton ；其等電點pi = 8 . 6 0 . 3 。
  11. A new method of two - dimensional electrophoresis is introduced, and applied to analyze protein subunits

    摘要介紹了還原條件與非還原條件雙向電泳法，並應用此方法研究了種子蛋白質亞基結構。
  12. In this study, the profiles of proteins expesssion in different growth phases of rt19 have been obtained using the technique of proteome analysis with two - dimensional polyacrylamide gel electrophoresis ( 2 - d page ). an efficient imagemaster 2d was used to reveal the number of protein spots corresponding from lag phase to stationary phase, varying from 398 to 516, which manifested kinetic state of proteome in cell

    本文採用高解析度的雙向電泳技術，得到不同生長時期的蛋白表達譜，應用高效的imagemaster2d軟體進行圖譜分析，結果顯示： （ 1 ）從延滯期到穩定期的末期，所表達的蛋白數量由398個逐漸增加至516個，顯示了細胞內的蛋白在不同生長期的動態表達水平。
  13. The lesions can be seen with mri scans, but the appearance in the csf of increased protein from igg that demonstrates oligoclonal bands on electrophoresis is very consistent with this diagnosis

    採用磁共振（ mri ）掃描可發現損害，腦脊液檢測結果為免疫球蛋白g升高，這表明電泳顯示的寡克隆帶與診斷是非常一致的。
  14. These essential component, hemagglutination, toxicity, immunogenicity of mycoplasma hyopneumoniae strain xy - 1 membrane protein were studied. the range of membrane protein mw is from 14kd to 100kd by means of sodium dodecyl sulfate - polyacrylamide gel electrophoresis, and molecular weights of the protective antigens were 36kd and 64kd by sds - page

    本研究以分離豬肺炎支原體野毒株（ xy - 1株）的膜蛋白為試驗材料，系統地研究了膜蛋白的分子量范圍、血凝性、毒性及免疫原性等。
  15. The results were as following : 1. construction and identifcation of recombinant plant expression vector pbi ! 2i - th by dna recombination technology, sweet protein thaumatin gene was cloned into plant expression vector pbii2i. recombinant plasmid pbii2i - th was constructed successfully by enzyme cutting and electrophoresis

    甜蛋白thaumatin基因植物表達質粒pbi _ （ 121 ） - th的構建與鑒定利用dna重組技術，將植物甜蛋白thaumatin基因克隆至植物表達載體pbi _ （ 121 ）中，通過酶切、電泳，鑒定thaumatin基因已成功構建到植物表達質粒pbi _ （ 121 ）中。
  16. Cereals and feedstuffs for import and export - identification for barley variety. method of protein electrophoresis

    進出口糧食飼料大麥品種鑒定.蛋白質電泳分析法
  17. Application of protein electrophoresis technique in identification of maize seed purity

    蛋白質電泳技術在玉米雜交種子純度鑒定中的應用
  18. The crosslinking products of several bifunctioanal crosslinkers with different length of arm spacer and different solubility to water or lipid were detected by sds - page and then western blotting or two - dimension electrophoresis. it was found that 33 kd protein can be crosslinked with cp47, cp43 or d2 by edc ; cp47 dimer and crosslinking between cp43 and d2 occur when egs was applied ; treatment of psil core complex with dtsp turn out the products from 33 kd protein, di and the components of lhcii, those comprised by di and the components of lhcii, and those constituted by cp29, psb s and lhcii. the above results suggest that the protein subunits existing in the same crosslinking products are located closely in the psil core complx, which indicate the relationship of their functions

    結果顯示： 33kd蛋白能與cp47 、 cp43和dz蛋白交聯；在交聯劑egs作用下cp47形成二聚體和發生dz與cp43的交聯；在dtsp處理下， 33kd蛋白、 di蛋白、 cp29和部分lhcll組分形成110kd左右的交聯帶， d ；與部分lhcll組分組成了55kd交聯帶， cp29 、 psbs及部分lhcll組分組成了45kd交聯帶c說明上述psll放氧核心復合物蛋白組分在空間位置上的鄰近關系，也暗示了它們在功能卜的聯系。
  19. Indentificatiort is also the first step towards studies on protein co - and post - translational modification, and ultimately, function. in the present study, the total proteins of the photo - thermo sensitive genie male - sterile rice ( oryza sativa, peiai64s ) spikelet at meiosis stage were used as the material. by optimizing crucial factors and procedures such as sample treatment, electrophoresis parameters, and gel concentration, 2 - d maps with high quality and reproducibility were obtained

    用兩種方法對經雙向電泳分離的凝膠上的蛋白質點進行了初步鑒定，一是通過電印跡轉移把蛋白質轉到pvdf膜上，再用edman降解的方法測得部分相對分子質量在10000 - 30000da的蛋白質點的n -端序列，通過網上搜索其同源性對其進行鑒定，並確定該點在凝膠上的位置。
  20. Proteomic analysis was performed by two - dimensional protein gel electrophoresis and subsequent computerized gel analysis for detection of distinguishing patterns of protein expression

    蛋白質組學分析是由二維蛋白凝膠電泳和隨后的計算機輔助凝膠分析並發現差異表達的蛋白。
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