electroporation 中文意思是什麼

electroporation 解釋
電穿孔術
  1. Construction and expression of yeast engineering yaccine : s14 / gnsag " as transformed to yeast host straln x33 by means of electroporation after ppiczaa s, . aresag " as l ined by saci enzyme. the single fungus, as choose and dibble inocu1ating in we and am plate, the positive fungus was gro ' ing in rm but not in w, and was 6 inoculated in ypd which included zeocine 500ug / ml and 1000ug / ml. 5 transformers were ampl if ied by pcr, three is same with positive control

    選取單個菌落分別點種到刪平板和md平板，找出在回d上生長正常， w上生長緩慢或不生長的菌落，即陽性菌落，再以陽性菌落分別塗布zeocine含量500ug加， 1000ug砌l的ypd平板，以高濃度的抗生素篩選高拷貝的酵母工程菌，在含500ug ml高濃度抗生素平板上獲得了15個轉化子，取其中5個進行pcr擴增，有3個擴增產物與陽性對照相同，說明此酵母細胞中已含有s hbsag融合片段，其中之一命名為p
  2. We used fission yeast schizosaccharomyces pombe ( s. pombe ), an unicellular eukaryotic organism, as research material. electroporation was adopted to load ca2 + fluorescent indicator into yeast cell and under the laser scanning confocal microscopy ( lscm ), we observed cytosolic ca2 + distribution and relative content as well as fluorescence intensity of gfp - cam in different phases of cell cycle of yeast cell. flow cytometry provided a way of determining the relative dna content of populations of fission yeast

    本文以單細胞的真核模式生物裂殖酵母（ schizosaccharomycespombe ）為研究材料，通過激光掃描共聚焦顯微鏡觀察酵母細胞胞質內游離ca ~ （ 2 + ）的分佈及相對濃度，以及不同周期時相細胞中gfp - cam的熒光強度變化，並採用細胞流式法對酵母細胞的相對dna含量進行測定以確定細胞所處周期時相。
  3. Israelensis recombinants, which contained recombinants plasmid pmt4 and pmt9 respectively, were obtained by electroporation. the bioassay results showed that the recombinants b - pmt9 and b - pmt4 had toxicities both to resistant and susceptible c. quinqnefasciatus larvae during vegetative growth stage, having the lc ? o values similar to that of. fi. sssii - 1. however, the toxic levels of the final sporulated cultures of recombinants b - pmt4 and b - pmt9 differed, with a lcso value of 2 49mg / ml for b - pmt9 and little toxicity for b - pmt4 by using the plasmid pmt9, m txl gene from b. sphaericus was ligated with p20 and cytjaa gene, giving recombinant plasmid pmpx2

    含有pmt9和pmt4的大腸桿菌轉化子能表達產生mtx1毒素，發酵液對敏感和抗性致倦庫蚊幼蟲具有中度毒殺作用；含有pmt9和pmt4的蘇雲金芽孢桿菌轉化子b - pmt9和b - pmt4在營養體生長階段對敏感蚊幼和抗性幼蟲也具有毒性，毒力與野生型b . sss - 1相當，而不同轉化子在芽孢形成期的毒力因插入的mtx1基因轉錄方向不同而表現出差異，其中b - pmt4對目標蚊幼毒力極低（ lc _ （ 50 ） 10mg ml ） ，而b - pmt9對蚊幼蟲具有毒性（ lc _ （ 50 ） = 2 . 49mg ml ） 。
  4. The artificial antibacterial peptide - ceme gene was designed according to the codon with the highest frequency in pichia pastoris, then the ceme gene was integrated into the chromosome of pichia pastoris strain - gsl 15 by electroporation

    我們根據人工設計的新型抗菌肽ceme序列設計ceme基因，重組到酵母胞內表達型的整合載體phild2中，通過電轉化作用將ceme基因整合至酵母宿主gsll5染色體上。
  5. Finally, the chimeric gene was inserted into binary ti vector plbj21, and cloned into agrobacterium tumefaciensgv3101 by electroporation

    為了提高can小肽表達強度，本實驗構建了can小肽二連體。
  6. Tagging cells with quantum dots by electroporation

    電穿孔法在量子點標記細胞中的應用
  7. Analysis of the electroporation mechanism under the pulse electric field

    脈沖電場致細胞膜電穿孔的機理分析
  8. Progress of gene transfer technology of crop seed mediated by electroporation method

    電激法介導作物種子基因轉移的研究與進展
  9. Electroporation of sperm to introduce foreign dna into the genome of pinctada maxima jameson

    大珠母貝精子介導外源基因轉移研究
  10. After the plasmid was assayed for dna sequence, it was transformed into gs115 by electroporation

    利用限制性內切酶分析、 a序列測定在a水平對質粒進行鑒定。
  11. 2. we general introduce the principle of microwave disinfection and the mechanism of electroporation

    2 .詳細地介紹微波滅菌的基本原理，及與本文密切相關的電穿孔機理。
  12. It was then cloned to the secreted vector - ppic9k and recombined successfully into the chromosome of pichia pastoris host strain - gsl 15 by electroporation

    通過電轉化作用該基因片段被成功地整合至酵母cs115的染色體上，經過甲醇誘導之後，該基因得到了分泌表達。
  13. In the direction of electromagnetic theory and the l of the mechanism of electroporation, the coaxial cavity resonator is be built by the means of cst microwave studio ?

    以電穿孔理論和電磁理論為基礎，採用cstmicrowavestudio ?建立電容加載同軸諧振腔的三維電磁模型，闡述基於此的滅菌方案。
  14. After linerization, the recombinant plasmid was transformed into pic hia pastoris by electroporation, which then were cultured in md plate free of histidine, from which the positive colones were propagated

    重組質粒線性化后，用電擊法將重組質粒轉化入巴氏畢赤酵母，在缺組氨酸的md板上篩選陽性菌落，然後用不同濃度的g418 ? ypd板篩選多拷貝插入單菌落。
  15. Transformation was done by electroporation. human fl extracellular domain cdna transformed to yeast host strain km71, then his + muts phenotype transformant was screened out and cultured in flasks, and rhfl was expressed under the induction of 0. 5 % methanol

    我們提取了km71ppic9k - fl轉化菌株的基因組dna進行southern實驗，檢測目的基因的整和插入；提取總rna ，進行了northern實驗，檢測fl基因在轉化菌株中的表達。
  16. The essentially universal biophysical phenomenon of " electroporation " occurs if an appropriate pulse field is applied. electroporation is believed to be the rapid creation of aqueous pathways through lipid - containing barriers in cells and tissue. the driving force is the physical interaction of electric fields with different dielectric constants

    電穿孔效應是指在適當高壓脈沖電場作用下，細胞或組織間起相對隔離作用的「屏障」內快速形成液態通道的現象，是電場與具有不同介電常數而且易變形的物質相互作用的結果。
  17. And the transformation efficiency of enterococcus faecalis is obviously higher than that of lactobacillus plantarium by pulse electroporation with different voltages, which suggests th at the two strains of lab have different usage in the research of genetic engineering of lab

    利用不同的電壓進行脈沖轉化兩株乳酸菌，發現兩株菌的轉化效率和電壓有對應的正相關，並且糞腸球菌的轉化效率明顯比植物乳桿菌高。
  18. The e2 genes above of the prevalent strain ( guangxi yulin strain ) were cloned respectively into secreted expression vector ppic9k of eukaryotic expression system p. pastoris and transformed into p. pastoris by electroporation after linearization, 25 high - copied transformants were obtained by g418 screening. it was proved that the e2 genes were integrated stably into chromosome of p. pastoris by dot blot and dna sequencing

    豬瘟病毒e2基因的真核表達：分別將csfv兩個代表株的e2基因克隆入畢赤酵母（ p . pastoris ）分泌型表達載體ppic9k中，酶切線型化后電穿孔導入p . pastotis進行整合，經g418篩選得到25個高拷貝轉化子，經dna斑點試驗和dna測序證明外源基因e2穩定地整合到p . pastoris染色體中。
  19. These vectors were tried to transform into the p. digitatum imazalil - sensitive isolate by the methods of peg - mediated or electroporation transformation. after that, the transformed protoplasts were regenerated on czapek medium containing hygromycin at 300 # g / ml. unfortunately, the ideal transformants could not be obtained

    採用peg法和電擊法兩種方法進行指狀青黴原生質體的轉化，然後將得到的轉化產物轉移到查氏再生培養基中培養，遺憾的是沒有得到理想的轉化子。
  20. The very plasmid pbmb0474 was constructed. when the recombinant plasmid pbmb0474 was transferred into crystal negative bt strain bmb171 through electroporation, the expression of the fusion genes about 150kda - 160kda can be detected by sds - page. 4

    將pbmb0474電轉化到bmb171中得到蘇雲金芽胞桿菌重組菌， sds - page結果顯示重組菌可以表達大小約150kda - 160kda的gfp融合蛋白。
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