env gene 中文意思是什麼
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Envelope gene gp85 of imc10200 subgroup j avian leukosis virus was cloned and expressed in the present study. the sequence encoding the gp85 domain of imc 10200 alv - j was amplified from pgem - imc2. 2 vector, which contains env gene of alv - j imc 10200 strain, and cloned into transfer vector pfast bacl
為深入探討alv - j的亞群特性，本研究利用alv - jgp85基因兩側的序列片段為引，物從正常spf蛋雞、商品肉雞和df1細胞基因組中完整地擴增了內源性類alv - jgp85基因。 -
The env protein deduced from env gene encodes the hydrophilic surface protein ( su ) and the hydrophobic transmembrane domain ( tm ) that determine the specific interaction between virus particles and cell surface receptors during retroviral entry. the su of retroviruses is a highly variable genetic element, containing receptor binding sites and major antigenic determinants. exjsrv - specific dna probes were derived. by using these dna probes in tissue hybridization. we successfully identified jsrv mrna expression and proviruses dna in sheep lung tissues infected with jsrv and control group has no postive signals, validating the use of exogenous virus - specific dna probes in the analysis of oncogenic proviral integration sites and identification of integrated exogenous proviral sequences
用地高辛隨機引物法標記exjsrv特異的env片段，制備探針，原位雜交檢測spa肺組織中的rna及前病毒dna ，結果表明spa患羊肺組織內有jsrvenv基因mrna的表達，同時也檢測到了前病毒dna ，而相應的陰性對照卻無陽性信號，證實外源性病毒特異的dna探針在致瘤性前病毒的整合位點和整合的外源性前病毒的檢測中具有可信度。 -
Molecular epidemiology analysis of hiv in shenzhen and replication analysis between hiv - env gene variation and epidemic time
膜蛋白基因變異與感染時間相關性分析 -
For the first time, the complete long env gene of the jaagsiekte sheep retrovirus ( jsrv ) has been colpned from provimses in china in sheep genome and sequeneed, the gene is 1836 nucleotides long. there are 12 nucleotides mutations compared with type i and type ii jsrv counterparts the sequences of the virus was 87 % to 88 % identical to the described three jsrv env sequences
將pcr產物純化，並與puem - t載體相連，轉化大腸桿菌，經pcr ，酶切鑒定及序列測定，得知所得env片段與型jsrv同源性為88 ，與型jsrv同源性為87 ，證明所克隆基因為外源性目的基因，至此，我們在國內首次獲得了env陽性克隆。 -
The results showed that the two isolates have more than 95 % homology with the snv strain. the env gp90 was amplified from the snv strain by pcr and was cloned into the downstream of gst gene in pgex - 6p - l vector according to the correct open reading frame, and this recombinant was transformed into bl21 for expression
結果發現，在核苷酸水平上，參考株snvenv基因與兩株中國分離株的同源性分別為97 . 7和95 ，而在氨基酸水平上，其同源性為96 . 9和92 . 7 。
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