enzymatic digestion 中文意思是什麼
enzymatic digestion
解釋
酶消化-
In the first trial, combination of enzymatic digestion was used to prepare suspensions of spermatogenic cells from adult mouse testis, and then a modified discontinuous percoll gradient centrifugation method ( 15 %, 22 %, 30 %, 40 %, 50 %, 60 % ) was introduced to isolate spermatids from the cellular suspensions. the content of spermatids in each isolated fraction by percoll method was determined by morphology ( wright - giemsa stain ) and flow cytometry analysis, and the viability of spermatogenic cells was assessed by using eosin y exclusion test
在第一部分試驗中,首先利用連續3次組合酶消化成年小鼠睪丸制備睪丸細胞懸液,然後經6層非連續percoll梯度離心法( 15 、 22 、 30 、 40 、 50和60 )分離,通過形態學和流式細胞術鑒定南京醫科大學碩士學位論文各個percoll組分中精子細胞的含量,並以伊紅y排斥試驗測定細胞的存活率。 -
Determination of disulfide bonds in protein by enzymatic digestion and mass spectrometry
酶切質譜法測定蛋白質的二硫鍵 -
It did not cause any death or side effects on chickens orally dosed at or below 108 cfu. at week 2 after the 2nd immunization, no s. typhimurium cells were recovered from the spleen and liver. zj111 / pcdna3 - f showed high stability in vitro and in vivo even in the absence of ampicillin as determined by enzymatic digestion and pcr
上為了進一步探討利用減毒鼠傷寒沙門氏菌為載體傳遞ndvdna疫苗的免疫原性,將zjlll pcdna3f菌株分別給3日齡非免疫雞以10 、 fu 、 10 、 fu和10 、 fu的劑量進行首兔, 2周后h免, h免后4周攻擊強毒株f 。 -
As a result, more than 97 % of the testicular cells remained their viability after enzymatic digestion
結果組合酶消化后獲得的睪丸細胞懸液中, 97以上的細胞仍然存活。 -
Most commonly used is the mechanical method in which a small piece of testicular tissue is torn apart using needles and the spermatids are released. while enzymatic digestion of human testicular tissue can result in a mixture of cell types including a whole range of spermatogenic cell types
最常用的是機械方法,即利用細針將活檢獲得的小塊睪丸組織撕開,釋放出精子細胞;也可利用酶將睪丸組織消化成細胞懸液,其中包含所有類型的生精細胞。 -
So a lagre populations of relatively purified spermatids can be isolated from mouse testis by enzymatic digestion combined with discontinuous percoll gradient centrifugation method
因此採用組合酶消化結合非連續percoll梯度離。 g法,可有效地從小鼠睪丸中分離到較多較純的存活精子細胞。 -
All study aim is to lay a foundation for clinic appliance. methods : 1 ) hpfl was isolated by enzymatic digestion derived from rat fetal liver on ed13. 5d. furthermore, erythrocyte and other cell were removed from hpfl by erythrocyte - cracking solution and different attachment method
研究方法: 1 )取ed13 . 5d的大鼠胎肝,酶消化法離散細胞,用紅細胞裂解液去除紅細胞,差速貼壁法去除其它細胞,接種于不同的基質和培養液中, mtt法比較不同培養液和培養基質對大鼠hpfl的體外生長影響。
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