enzyme restriction analysis 中文意思是什麼
enzyme restriction analysis
解釋
酶限制分析法- enzyme : n. 【化學】酶。 digestive enzyme 消化酶。 induced enzyme 誘導酶。
- restriction : n. 1. 限制，限定。2. 拘束，束縛；自製。3. 【邏輯學】限定。
- analysis : n. （pl. -ses ）1. 分解，分析；【數學】解析。2. 梗概，要略。3. 〈美國〉用精神分析法治療（＝ psychoanalysis）。
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Based on the structure and function analysis of hirudin, a potent thrombin inhibitor, and some platelet aggregation inhibitors, which contain the recognition sequence argglyasp as their functional motif, two chimeric antithrombotic molecules were designed by introducing rgd sequence to hirudin cterminus. these chimera genes were constructed by pcr and inserted into the expression vector pet21a, the constructs were confirmed by restriction enzyme digestion and dna sequence analysis. these recombinant plasmids were transformed into
經限制酶消化和dna序列分析，證明兩種重組質粒與設計完全一致。由於rgd -水蛭素嵌合基因上游連接了金黃色葡萄球菌蛋白a spa的信號肽序列，在iptg誘導下兩種嵌合分子都獲得了分泌表達，表達產物主要集中在細胞周質空間。 -
It was confirmed by restriction enzyme digestion analysis that egfp fusion expression plasmids of scfvs were successfully constructed
的序列正確，經酶切鑒定證實成功地構建了綠色熒光蛋白基因融合表達載體。 -
Abstract : the polymorphism of angiotensinogen gene at position 174 was studied in 90 cases of essential hypertension patients and 109 controls by pcr, restriction enzyme analysis and electrophoresis methods. the results showed the distribution of genetypes in hypertension group was significantly different from that of controls group. this suggested there is a correlation between the variant of agt174 and hypertension
摘要本文採用pcr 、限制性酶切和電泳分型等方法，分別對90例原發性高血壓患者和109例正常人血管緊張素原基因多態位點agt174進行了檢測，結果表明，高血壓組中三種基因型的分佈與對照組顯著不同，提高該位點變異與原發性高血壓的發生相關。 -
Random analysis of 13 clones with enzyme restriction showed that 10 plasmids in the clones contained 300 - 600 bp inserts
胭p二:廠。施二中nadp一蘋果酸酶活性高的多。 -
The ha 1 gene was inserted into the bacterial plasmid pgex - 4t - 2 and the recombinant plasmids containing ha 1 gene were identified by restriction enzyme analysis and pcr mathod
結果表明同源性分別達到98和97 ，並且在ha1切割位點有多個堿性氨基酸的插入序列，證明其為強毒株。 -
Acetylornithine deacetylase is the key enzyme of producting l - methionine. we mainly do research work on the construction of acetylornithine deacetylase gene - engineering strain and characteristic of proteinase. in order to get high expression deacetylase strain, we obtain the gene by pcr arge gene. the product ( 2800bp ) was cloned into puc19 plasmid and confirmed with blue / white dot screening > restriction enzyme analysis and pcr. then taking the nucleotide sequencing compared with the sequence at blast of u. s. a. we constructed a high expression of gene - engineering strain - pxj 128 which containing the arge gene on the high expressing system of pxji18 with activity of acetylornithine deacetylase above 20000u / g
為了獲得高效表達的脫乙酰鳥氨酸酶工程菌株，在工程菌技術改造及其固定化研究做了進一步的研究和探討。我們採用基因工程技術，通過pcr技術擴增出了酰化酶關鍵酶基因?脫乙酰鳥氨酸酶基因arge ，將其克隆到puc19載體中，經酶切鑒定、 pcr鑒定篩選出重組陽性質粒，並測序鑒定，通過美國blast程序進行了基因數據庫相似性比較分析。 -
First, a pair of pcr primers was designed to isolate fmdv - vp1 gene according to the published fmdv - vp1 sequence. after pcr of dna isolated from the tissues, the fmdv - vp1 gene was cloned into cloning vector. positive clones were analysed with restriction enzyme digestions and further identified with sequence analysis
首先，根據已知的fmdv基因序列，設計特異性引物，擴增出fmdv - vp1基因，將克隆到的vp1基因連接到測序載體上後用dna測序儀對該序列進行測序。 -
374 - nt sequence analysis between nt 47 - 420 and restriction enzyme ( re ) clevage site mapping of f gene between nt 34 - 1682 were used to compare the 18 isolates for genetic analysis. a phylogenic tree was constructed based on the 374 - nt - sequence data of eighteen isolates in the study and 37 ndv reference strains from genbank and published resources
通過dnastar軟體對f基因47 470nt間片段進行同源性分析比較並繪制了遺傳進化樹枝狀結構發生圖，結合334 1672nt間三種限制性內切酶（ re ： hinf ， bsto及rsa ）位點的分佈情況，確定了這些分離株的基因分類地位。 -
It has made the strong basis for further studying mechanism of replication, virulence and determinant, attenuation, pathogenesis, functions of genetic products, specific diagnosis, cell and host tropism, development of dna vaccine and marker vaccine of csfv, and provided an excellent tool for molecular virology. main research contents include : based on published nucleotide sequences of csfv and by the help of computer analysis software, high conservative regions and single restriction enzyme sites of genome were selected. utilizing rt - pcr and nested - pcr techniques, 7 overlapping cdna fragments covering the full genome of csfv c - strain were successfully amplified
中國豬瘟兔化毒（脾淋毒）基因組cdna文庫的構建、序列分析：根據已發表的豬瘟病毒（ csfv ）核苷酸序列，藉助計算機軟體分析，選擇高保守區段和基因組中的單一限制性酶切位點，利用rt - pcr及nested - pcr和helf - nestedpcr技術，成功地擴增出了覆蓋c -株全基因組的7個cdna重疊片段f1 f7 ，分別克隆到pmd - 18t或pgem - teasy載體進行測序后，拼接出了其核苷酸序列。 -
For the products of primary rt - pcr and nested - pcr are all encompassing the hyper - variable region of vp2 gene, so we can take a restriction enzyme analysis ( rea ) directly
第二，本研究的基礎rt - pcr和nested - pcr所擴增的片段均是橫跨ibdv的vvp2的，所以，可直接對pcr產物進行酶切分型研究。 -
Proper multi - copy gene was selected and cloned into puc19 vector. restriction enzyme analysis and dna sequencing confirmed that 5 - copy gene was correctly inserted into the vector
選取合適拷貝數的串連重復基因，將其克隆至puc19載體，雙酶切、 pcr擴增和dna測序證明串連重復基因構建成功且基因方向相同。 -
We confirmed the correct construction by pcr and restriction enzyme analysis. in this research, hypocotyls were used as the explant and several factors affecting genetic transformation of carrot mediated by agrobacterium tumefaciens were studied
利用vp7基因和質粒pbi121上相同的單克隆位點，將vp7基因定向克隆到植物表達載體pbi121上，構建了pbi121vp7表達載體。 -
Conclusion : by restriction enzyme secting, ligating, transforming, restriction enzyme analysis, and final dna sequencing, the pbd - i and pbd - ii gene were proved to be recombinated with the expression vector and the recombinated vector ppd - 1 and ppd2 were transformed successfully
結論：經過酶切、連結，構建成重組質粒ppd上、 ppd上，再經轉化、抽提質粒及酶切分析，最後經dna測序證實， rticr擴增的pbd i 、 pbd 11基因與piflpdt 」 xsi表達載體構建成功。 -
After the pbd i and pbd ii gene ligated with the expression vector pinpoint ? a - 3, the recombinated plasmid ppd - 1 and ppd - 2 was transformed into jm109 strains, 4 positive clones were screened by restriction enzyme analysis, dna sequencing showed that two out of 4 positive clones inserted sequence of the constructed plasmid, which was the same as that of pbd - i and pbd - ii gene respectively, and its reading frame was correct, thus its could be used to express fusion protein
將pbd - 、 pbd -基因與表達載體pinpoint ~ （ tm ） xa - 3連結后獲得的重組質粒ppd - 1 、 ppd - 2轉化于大腸桿菌jm109中。經抽提質粒、酶切分析及pcr擴增，分別篩選到4個陽性克隆，將其中二個陽性克隆由測序分析，證實1個含pbd i基因片斷， 1個含pbd 11基因片斷，且閱讀框正確，可用於融合蛋白表達。 -
Ii ) two fragments ( about 240bp and 130bp ) were amplified from human keratinocytes total rna by rt - pcr. the recombinant pm - hpabl and pm - hpabs plasmids were constructed by inserting 240bp and 130bp pcr products into pmd 18 - t vector, respectively. the recombinants were identified by restriction enzyme analysis and dna sequencing, iii ) two orfs ( > 100bp ) were found in the insert sequence of pm - hpabl
應用smartpcr試劑盒和簡並引物從人皮膚角質形成細胞cdna中擴增到長分別為24obp和13obp左右的2種片段，將它們插入pmd18一t載體，用酶切法初步篩選陽性重組子pm . hrabl和pm一hrabs ，對陽性重組子進一步作測序鑒定。 -
The orf of the hbrp coding a peptide of 233 aa, using the dnasis v2. 5 demo analysis its structure and pi 3. screening 1 ) screening using restriction enzyme
Pcr產物和pgex一sx一1融合表達載體分別用bamhi和x五01限制性內切酶雙酶切后連接，構建融合表達載體。 -
After enzyme restriction and sequencing analysis, the nucleotide data had been further analyzed by antheprot 5. 0 and clutalw softwares. the analysis results showed that the cloned dna fragment had a longest open reading frame ( orf ) of 1035nt, it predicted to be encoded a 344 - aa protein with the molecular weight of 36kda
應用antheprot5 . 0 、 clustalw等分子生物學軟體分析，顯示主要外膜蛋白前24個氨基酸是較強的疏水性區域，可組成信號肽，其與omp基因的同源率達96 ，氨基酸的同源率高達98 。 -
Both genbank blast search and restriction enzyme map analysis reveal that the sequences of bdnf mature peptides from different species are highly conserved during evolution
應用系統發育和進化樹研究的philip軟體包，以及bdnf基因編碼區序列，對20種動物在分類地位上進行探討。 -
Restriction enzyme digestion analysis and pcr analysis showed that these two plant expression vectors constructed were correct. 49caixin, one cultivar of the brassica campestris l. ssp. chinensis, was transformed by vacuum infiltration method
通過真空滲入和花序浸漬法，將上述雙抗和單抗基因分別導入白菜不結球類型? ? 49菜心中，獲得了7棵抗性株。
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