expected vector 中文意思是什麼

expected vector 解釋
期望矢量
  • expected : 期待,期望
  • vector : n 1 【數學】向量，矢量，動徑。2 【航空】飛機航線；航向指示。3 【天文學】幅，矢徑。4 【生物學】帶...
  1. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  2. Full - length or truncated cdna was subcloned into prokaryotic expression vector pet30a and expression induced in e. coli bl21 ( de3 ). no squalene synthase polypeptide of expected molecular mass was observed in e. coli containing the putative full - length squalene synthase cdna, however, overexpression in e. coli was achieved by truncating 30 hydrophobic amino acids at the carboxy terminus

    但在含有全長的鯊烯合酶cdna的大腸桿菌中並沒有觀察到預期大小的鯊烯合酶表達，而c末端截短30個疏水氨基酸的鯊烯合酶可在大腸桿菌中過量表達。
  3. For desired gene replacement, hyg / cml and str / spc gene cassettes were also incorporated into the new vector system. it is expected that hygwould be integ

    為了驗證新型yac載體系統的功能，我們將phz621 ~ phz622進行了適當地改造，構建了質粒phz625 。
  4. In this paper, a method to evaluate hardware performance of laser detection system with the array signal estimation is proposed. and the optimally weight vector of array signal can be acquired by the doa estimating of received signals. in order to acquire the maximum output power of expected signals, summation of weight vector is applied

    提出用陣列信號源的估計來判斷激光檢測系統硬體性能；用接收信號波達角的估計得出陣列信號最佳權向量；用權向量的加權求和獲取期望信號的最大輸出功率，同時基於davidl . donoho軟閾值理論，進行多層小波降噪，重構原始路面信號。
  5. Vector modulators have been used in this paper to vary the phase and amplitude of signals in the in - band spurious cancellation loop of feed - forward power amplifiers ( ffpa ). in the two - tone test, 3. 5ghz ffpa developed in this paper shows more than 35db improvement in the third - order inter - modulation ( im3 ), offers approximately - 60dbc im3 output, achieves the expected goal, and meets the communication requirement

    本文研製的3 . 5ghz前饋放大器採用了矢量調制器來實現前饋環路的幅度和相位調節，在雙音測試結果中，三階交調im3的改善超過了35db ，前饋系統輸出im3抑制近- 60dbc ，達到了預期指標，滿足了通信需要。
  6. Cloning and analyzing of the full - length cdna sequence the fragments consistent with expected length from the product of race - pcr reaction are separated, purified and recovered, after which the fragments are cloned into the vector of pmd - 1st to transform the competent cells

    山na的克隆和測序將得到的3 』一及5 』 scepcr產物符合預期長度的片段，分離后純化、回收，克隆到ta克隆載體pmd 18t ，轉化e
  7. The amplified e2 fragments of two hcv strains were all 1280bp in length by 2 % agrose gel electrophoresis. expected size of 1280bp of 2 fragments and their specificity were confirmed by restriction endonuclease digestion and then they were cloned respectively into pmd - 18t vector

    以此病毒rna為模板，利用rt - pcr技術，擴增出了豬瘟野毒株hcv - jn 、 hcv - yc完整的e2基因，用pmd - 18t載體克隆，經電泳檢測、酶切分析及pcr鑒定初步證實了所擴增片段的特異性。
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