expression system 中文意思是什麼
expression system
解釋
表達系統- expression : n 1 表現，表示，表達。2 詞句；語句，措辭，說法。3 表情，臉色，態度；腔調，聲調。4 【數學】式，符...
- system : n 1 體系，系統；分類法；組織；設備，裝置。2 方式；方法；作業方法。3 制度；主義。4 次序，規律。5 ...
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Construction of an expression system of epstein - barr virus thymidine kinase tk
的克隆及表達載體的構建 -
With the successful expression of exogenous gene in plant. the advantage of plant expression system become a highlight increasingly. based on the successful expression of hbmp - 3m gene into tobacco, we maked the study o f transferring hbmp - 3m gene into tomato and hbmp - 3 gene into tobacco, in order to obtain tomato transgenic plant with hbmp - 3m gene and tobacco transgenic plant with hbmp - 3 gene, to establish basis for getting step farther of leaning the expression of hbmp - 3m gene and hbmp - 3 gene in plant, the difference between the product of hbmp - 3m gene and hbmp - 3 gene in plant and the active of expressible product
隨著植物基因工程的迅猛發展，一些疫苗、抗體、細胞因子等異源蛋白在植物中的成功表達，植物表達系統的優點日益受到關注。本研究室在成功地將hbmp - 3成熟肽基因轉入煙草並檢測有目的蛋白表達的基礎上，進行hbmp - 3成熟肽基因轉化番茄和hbmp - 3全長基因轉化煙草的研究，以期獲得轉hbmp - 3成熟肽基因番茄和轉hbmp - 3全長基因煙草植株，為進一步研究hbmp - 3成熟肽基因和全長基因在植物體內的表達及區別奠定基礎。 -
Fusion protein expression system can overcome those problem, increased the yield of yield of recombinant protein in e. coli. this remarkable increase in protein yield was thought to be due to protection of the target protein from proteolysis, improved folding, and efficient mrna translation. fusion protein also make the detection and purification easy, is a good strategies for achieving high - level expression of genes in escherichia coli
小分子量異源蛋自在人腸桿菌的表達受mrna不穩定、翻譯起始效率低、易被蛋自酶降解等因素的干擾，較難獲得高效表達，通過與已高表達的蛋自融合表達可以克服以上問題，可以使大多數蛋白獲得高效表達。 -
Construction of eukaryotic expression system of wild type and g88c mutant human - synuclein
四環素負調控表達模式對肺癌細胞體外生長的影響 -
They are also the key components of insect - baculovirus expression system ( bevs ), which has been one of the four main expression systems in gene engineering
同時，桿狀病毒昆蟲細胞表達系統是一類重要的真核表達系統，現已成為目前基因工程四大表達系統之一。 -
The reasults are summed up as following : 1 the study on chromosomes and mitoses of bmn cells the cell line, bmn, is a silkworm cell line widely used in silkworm molecular genetics, cell engineering, gene engineering and baculovirus expression system but whose genetics and cytobiology studies are nearly untouched. the chromosomes and mitoses of the bmn cells are researched by the air - drying method and culturing cells on cover glasses
同時，還通過原代培養實驗對新的家蠶胚胎細胞系的建立進行了探索和嘗試，並對家蠶胚胎原代培養過程中出現的細胞和組織類型進行了觀察、探討與研究。 1bmn細胞有絲分裂及染色體研究bmn細胞是家蠶分子遺傳學，細胞工程、基因工程和桿狀病毒表達系統中廣泛應用的家蠶細胞，但其遺傳學和細胞生物學背景知之甚少。 -
Human bone morphogenetic protein 3 is a member of tgf - b superfamily. lt can induce the differentiation of cartilage and bone tissue in mesenchymal cell. and is important to bone self - repairment and bone development during embryo morphogenesis. in addition, some other biological activities of hbmp - 3 have also been found. such as inducing development of embryo and stimulating differentiation of neural and blood cells. therefore, there is a great prospect in the use of hbmp - 3. there is trace content of hbmp - 3 in human body. it has been expressed in the expression system of eukaryotes and prokaryotes respectively, but its application is restricted because of defects in the process and modification after translation in prokaryotic cells and higher costs and lower yields existed in eukaryotic expression system
人骨形成蛋白3 （ hbmp - 3 ）屬于tgf -超家族的一員，可以誘導間充質細胞分化為軟骨和骨，在胚胎時期骨骼發育和骨再生修復中起著重要的作用，而且對胚胎發育過程中中胚層的誘導和分化、造血組織的發育以及神經系統的發育和修復等都起著重要作用，因而hbmp - 3有廣闊的市場前景。它在人體內含量極微，盡管研究人員已經在原核細胞和真核細胞表達系統中分別進行了表達，但是由於原核表達系統缺乏翻譯后的加工修飾，真核表達系統存在成本高、產量低等特點，限制了其在臨床上的應用。 -
It has been used to express in an attractive level a variety of intracellular and extracellular proteins of interest. ln our study, pichia pastoris gene expression system was used to express the soluble part of human trail ( tnf related apoptosis induced ligand ) and antibacterial peptide - ceme
因此它在基因工程方面的應用越來越受到人們的重視，已被廣泛應用於一系列胞內及胞外蛋白的高水平表達。我們利用這個系統分別對人的trail （ tnfrelatedapoptosisinducedligand ， trail acl2 ）基因可溶性部分和抗菌肽ceme基因的表達進行了研究。 -
Is encoded by grb ~ ast7. the expression analysis of the concatemer was proceeded in the e. coli pet expression system
本文主要工作就是構建grb - ast _ 7串聯體，將構建后的串聯體導入大腸桿菌進行表達分析。 -
Neuropeptide ast2 is encoded by grb - ast3. pcr methods were tried to construct grb - ast3 concatemer. the expression analysis of the concatemer was proceeded in the e. coli pet expression system
本論文採用改進的pcr 「自身模板-引物」法進行構建方法構建grb - ast _ 3串聯體，並將拼接好的串聯體轉入大腸桿菌進行表達分析。 -
Thinking of the cptl is small and its poor stability, a gst fusion expression system of pgex was used to improve the immunogenicity of cptl
考慮到cpti為小分子多肽，穩定性較差，為增加cpti蛋白的免疫原性，本研究採用了cpti與谷胱甘肽gst融合的pgex表達系統。 -
One was cloning, sequence analysis and expression of the fragment containing the b and c antigenic sites locating at the 5 " terminus in spike gene of tgev in prokaryotic expression system ( fused with gst ), the other was preparation of non - radioactive probe labeled by digoxigenin for detecting the rna extracted from tgev by assay of dot - blot
為了鑒別診斷tgev與prcv及對tgev進行流行病學調查，本研究採用原核表達系統（ gst融合表達系統）表達tgev纖突蛋白（ s蛋白）中含有b和c抗原位點的多肽，並且制備了非放射性地高辛標記的核酸探針，通過斑點雜交（ dot - blot ）檢測tgev核酸rna 。 -
The choice of an expression system for the high - level production of recombinant proteins depends on many factors
建立hpk - 5因子分離純化工藝； 4建立體外檢測復性蛋白質活性的方法。 -
This research focused on applying the system analysis principle and mathematical modeling technique to study knowledge expression system for crop cultivation management. based on extensively collecting, understanding, analysis, and integration of expert ' s knowledge and experience, literature and experiment data for cotton cultivation management, the dynamic relationships of cotton growth and management indices to variety types, ecological environments and production levels were quantified, and a dynamic knowledge model for cotton management ( cottonknow ) was developed. by further incorporating the rule - based knowledge system for cotton management, a comprehensive and intelligent knowledge model - based decision support system for cotton management ( kmdsscm ) was established with component design
本研究著重利用系統分析原理和數學建模技術來研究作物栽培管理的知識表達體系，在廣泛收集及充分理解和分析棉花栽培管理專家知識、經驗和資料的基礎上，利用棉花栽培理論與技術方面的現有研究成果，並結合必要的試驗支持，解析、提煉和綜合棉花生育及管理指標與品種類型、生態環境及生產水平之間的定量化關系，首次構建了棉花栽培管理動態知識模型，並進一步結合基於知識規則的棉花栽培管理知識庫表達系統，設計和實現了綜合性、智能化和構件化的基於知識模型的棉花管理決策支持系統（ kmdsscm ） 。 -
Expression system renders products that are free of post - translational modifications, it is likely that the binding of crylab toxin to bt - r3 protein via protein - protein interaction and does not require glycosylation
且該受體蛋白與cry1ab的結合部位位於bt - r3受體蛋白的胞外結構域。 bt毒蛋白與鈣粘蛋白受體的結合與受體是否糖基化無關。 -
Objective : construct high - level expression system of echistatin in e. coli methods : obtain amino - acid sequence of echistatin from genebank database. considering the bias of usage of 61 available aminoacid codons in e. coli, design the coding sequence of echistatin, synthesize the dna sequence chemically, get single copy coding gene and repeated two copy coding gene of echistatin. insert the sequence into expression vector pbv220, and more, we construct fusion expression clone of echistatin with pcr, identify the recombinant vector by dna sequencing
目的構建蛇毒鋸鱗蝰素（ echistatin ）的原核高效表達體系方法由genebank數據庫檢索蛇毒鋸鱗蝰素（ echistatin ）的氨基酸序列，結合大腸桿菌蛋白質合成體系對氨基酸密碼子使用的偏愛性，設計了echistatin編碼基因，體外人工合成編碼基因dna片段，通過適當的限制性內切酶位點插入表達載體pbv220 ，分別構建了echistatin的單拷貝表達克隆、雙拷貝串聯表達克隆；進一步通過pcr技術構建echistatin的融合表達基因克隆。 -
Therefore, this research is the first time to attempt to express porcine ifn - a ( poifn - a ) by eukaryotic expression system
鑒于以上研究現狀，本研究首次嘗試利用真核表達系統表達豬干擾素重組蛋白。 -
Construction of a high - effective expression system of bacillus subtilis recombined xylanase gene
幾丁質結合區三個結構功能域的鏈黴菌 -
We are now working on the expression and oral vaccination of the hepatitis b virus surface antigen and nucleic antigen by using the expression system in lab
正在利用這一表達體系研究乙肝表面抗原和核心抗原的在乳酸菌中表達及口服免疫。 -
In order to develop recombinant poifn - a preparations preventing porcine viral infectious disease, and to further expound the research of cytokines in the field of molecular biology, mature poifn - a was cloned and expressed in e. coli expression system in the study
為了在我國研製生物工程重組豬干擾素類制劑，防制豬病毒性傳染病和進一步開展豬干擾素分子生物學研究，本文進行了豬干擾素基因（ poifn ）的克隆、表達及其重組蛋白抗豬瘟病毒的研究。
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