filamentous actin 中文意思是什麼
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The his - tagged peacl - gfp purified from the supernatants could polymerize into green fluorescent filamentous structures with diameter, length and shape being identical to that of muscle f - actins, which could be labeled by tritc - phalloidin ( a specific agent for staining actin microfilaments ), and were identified as having a 9 nm diameter by negative staining, corresponding with that of the muscle f - actins ( 7 - 10 nm ). under polymerization conditions, his - tagged peacl - gfp polymerized with kinetics similar to those of skeleton muscle actin, that is, an obvious lag nucleation period at the beginning of polymerization and an s - like typical polymerization curve could be obtained. the critical concentration is 0. 75 umol / l near to that of chicken muscle actin ( 0. 56 umol / l ) under the same condition
熒光標記結合熒光顯微觀察表明：從可溶性上清中純化的his - taggedpeac1 - gfp聚合形成的微絲不僅可以直接在熒光顯微鏡下觀察，也可被微絲的特異標記物鬼筆環肽所標記，而且其直徑、長度以及形態上與已知的聚合肌動蛋白熒光絲一致；電鏡負染的結果進一步證實其直徑為9nm ，與傳統微絲直徑相當（ 7 ? 10nm ） ；聚合曲線有明顯的停滯期，為典型的s型聚合曲線，聚合臨界濃度為0 . 75 mol l ，這一結果與已有報道相似。
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