flanking fragment 中文意思是什麼

flanking fragment 解釋
旁側片段
  • flanking : 側面攻擊
  • fragment : n. 1. 碎屑,碎片,破片,斷片。2. 未完稿,斷簡殘篇。vi. ,vt. (使)成碎片,(使)分裂。
  1. Smai, kpni and sali flanking bamhi could be used to analyze the cloned fragment

    為應用協心加雙重報告基因研究根瘤菌的分子生物學積累了材料。
  2. The sequences flanking tn5 in magnetosome deleted mutant nm4 was cloned by anchored pcr, and was used as tag to extend the 5 ' and 3 ' terminal sequences of the target dna fragment by anchored pcr constinuously

    以tn5為標簽,用錨定pcr法克隆出突變株nm4的tn5側翼序列。並以此側翼序列為標簽繼續用錨定pcr法向兩邊延伸,克隆出一個5045bp的dna片段。
  3. The gd and ge gene was subcloned into puc18, resulting in pugdge. the fragment from pcdnas. 1 - including hcmv promoter / enhancer, mcs and neomycin resistance gene was inserted into the bamhi and bsteii restrication sites of pugdge, resulting in the universal transfer vector pgd - m - ge. the universal transfer vector pgd - m - ge has deleted the gi gene and 363bp in the 5 ' end of the ge orf of prv. there were 11 restrication sites for insertion of the foreign gene. the upstream and downstream flanking sequences were up to 1. 25kb and 1. 42kb. it will be useful for developing the recombinant prv expressing foreign gene ( s )

    將gd 、 ge基因連接于質粒puc18獲得pugdge ,缺失質粒pugdge的bamh和bste位點間391bp的片段。在此缺失位置插入來自質粒pcdna3 . 1 -的一偽狂犬病病毒gd 、 ge 、 tk基因的克隆與通用轉移載體的構建段含hcmv啟動子。多克隆位點和neo報告基因的片段,構建了通用轉移載體ppd m pe 。
  4. With h. trueperi genomic dna and degenerate primers, a 560 bp pcr fragment was obtained and labeled as a probe. after h. trueperi genomic dna was digested with different endonucleases, southern blot result showed a 2. 6 kb positive fragment digested by ecori and ipcr was carried out to obtain the flanking sequence

    將擴增片段用地高辛標記成探針,與用不同限制性內切酶完全酶切的h . trueperi總dna片段作southem雜交,結果顯示在ecori酶切片段的2 . 6kb處有陽性信號。
  5. Sequences flanking tn5 - 1063a can be recovered from the genome of mutant by excision, self - ligation and transfer to e. coli. the total dna of mutant was excised with ecori, which cut the genome frequently but not cut the transposon. after sequencing the self - ligated transpon, dna fragment flanking tn5 was obtained. the result showed 042bm - x1 contains a tn5 insertion in the gene smc00190, which function was unknown and was demonstrated to be related to salt tolerance by this study, and the gene was named as rst - 0x1

    通過ecori酶切突變株基因組,得到完整的tn5 (含有在大腸桿菌中起始復制的oriv )及其側翼的序列片段,該片段自連后轉化大腸桿菌,以tn5兩端已知的序列設計引物進行測序。 blast的分析測序結果表明, 042bm - x1和042bm - x2中tn5分別定位在苜蓿中華根瘤菌1021染色體上smc02682和smc00419基因內部,本實驗證明它們和042bm耐鹽相關,命名為rst - 0x1和rst - 0x2基因。
  6. Sequences flanking tn5 in nm21 was cloned by anchored pcr. a 3073bp fragment which contains three putative open reading frames ( orf ) and shows strong homology with the magnetospirillum magnetotacticum ms - 1 was obtained, the tn5 inserted in the orf1

    用錨定pcr法從nm21中克隆出tn5側翼序列,得到一個大小為3073bp的dna片段,此片段由3個orf組成,與m . magnetotacticumms - 1的對應片段具有89的同源性。
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