flanking gene 中文意思是什麼

flanking gene 解釋
側翼基因
  • flanking : 側面攻擊
  • gene : n. 【生物學】基因。 dominant gene顯性基因。
  1. Molecular cloning of 5 ' flanking region of ovine keratin associated protein 6 - 1 gene and comparison of the sequences

    端調控區的分子克隆及測序結果比較
  2. ( 3 ) at post - translation level plant mutual sequence of starting translation aaca and eukaryotic secretory signal peptide sequence was added to 5 ' - flanking region of t - pa gene and kdel ( sequence located to endoplastic reticulum ) to 3 ' - flanking region by pcr amplication and plant expression vector pbemt was constructed

    （ 3 ）翻譯后水平通過pcr擴增的方式在t - pa基因5端添加了真核分泌信號肽序列和植物翻譯起始共有序列aaca ，在3端添加了內質網定位序列kdel ，構建了植物表達載體pbemt 。
  3. In order to study the expression of 3 - defensins in liver as acute phase response proteins, a murine systemic acute phase responsive model was established by intraperitoneal injection o f lipopolysaccharide ( lps ) in our study. the mbd3 cdna sequence ( 145 - 169 bp ) was labled with [ - 32p ] atp as a probe to detect mbd3 mrna in different tissues by northern blot and analyze the time - and dose - dependant expression caused by lps. the 5 " flanking sequence ( - 167 - 179 bp ) of the mbd3 gene was designed as the probe and labled with [ - 32p ] dctp to investigate the binding of transcriptional factors to this region by electrophoretic mobility shift assay ( emsa ) and south - western blot

    以小鼠mbd3基因145 ? 169bpcdna序列合成探針，經[ - ~ （ 32 ） p ] atp標記后通過northernblot方法檢測mbd3在肝臟中的表達，同時分析了mbd3基因誘導表達的組織特異性，劑效和時效關系；結合mbd3基因啟動子區序列分析，以- 164 ? - 179bp雙鏈dna序列合成探針，經[ - ~ （ 32 ） p ] dctp標記后通過電泳遷移率改變實驗（ emsa ）和south ? westernblot方法對參與mbd3在肝臟中誘導表達調節的轉錄因子進行分析。
  4. We amplified aroa, aroc, arob of common pathway, csra and its flanking sequence of global regulation network from e. colt, and kan resistant gene from plasmid pet28a. 4. centerpiece of this

    在bioflo3000bateh / continuousbioreaetor （ sl ）進行發酵試驗結果證實，發酵液中副生雜酸較少，產品經初步純化后純度較高，可達93 % 。
  5. The analysis on the 5 " flanking region revealed tata box, putative api and nur77 response elements, prl and progesterone response elements motifs related with regulation on 20ahsd gene expression

    通過軟體對克隆到的5側翼區結構和功能分析，發現了20 hsd基因的tatabox 、 prl反應元件、類固醇激素反應元件、 ap1和nur77等轉錄因子結合位點。
  6. Phz621 - phz622, the new gene replacement vector system, had been constructed through the insertion of the 1. 0kb and 1. 4kb flanking sequences of hau3r gene from wild - type s. lividans in the same natural orientation

    預期在攜帶有大插入片段的基因置換yac分子進入野生型變鉛青鏈黴菌后， yac分子將通過1 . 4kb或1
  7. The research work was divided into 3 parts in this thesis : 1 ) 2. 5kb 5 " flanking region of the 20ohsd gene was isolated by pcr

    本研究工作主要分以下三個部分：第一部分運用pcr技術從大鼠promoterfinder文庫中擴增到了2 . 5kb的20 hsd基因5側翼區序列，並對其進行了序列測定。
  8. Firstly, the bovine as1 - casein 5 ' regulation region was cloned into the vector pegfp - 1 and constructed a new vector p - 5 ; then the bovine asi - casein 3 ' flanking region was inserted into the position of gfp gene in the vector of p - s and a mammary gland universal expressing vector pbcas was created

    首先將牛s1 ?酪蛋白的5 』調控區克隆到載體pegfp - 1上，成為載體p - 5 ；然後將牛s1 ?酪蛋白的3 』側翼區克隆到載體p - 5的gfp基因位置，從而構建成乳腺通用表達載體pbcas 。
  9. Gene promoter on its 5 flanking sequence

    基因5末端側翼序列啟動子的分析
  10. Gene promoter on its 5 flanking sequence analysis of the mouse

    基因5末端側翼序列啟動子的分析
  11. The gd and ge gene was subcloned into puc18, resulting in pugdge. the fragment from pcdnas. 1 - including hcmv promoter / enhancer, mcs and neomycin resistance gene was inserted into the bamhi and bsteii restrication sites of pugdge, resulting in the universal transfer vector pgd - m - ge. the universal transfer vector pgd - m - ge has deleted the gi gene and 363bp in the 5 ' end of the ge orf of prv. there were 11 restrication sites for insertion of the foreign gene. the upstream and downstream flanking sequences were up to 1. 25kb and 1. 42kb. it will be useful for developing the recombinant prv expressing foreign gene ( s )

    將gd 、 ge基因連接于質粒puc18獲得pugdge ，缺失質粒pugdge的bamh和bste位點間391bp的片段。在此缺失位置插入來自質粒pcdna3 . 1 -的一偽狂犬病病毒gd 、 ge 、 tk基因的克隆與通用轉移載體的構建段含hcmv啟動子。多克隆位點和neo報告基因的片段，構建了通用轉移載體ppd m pe 。
  12. Sequences flanking tn5 - 1063a can be recovered from the genome of mutant by excision, self - ligation and transfer to e. coli. the total dna of mutant was excised with ecori, which cut the genome frequently but not cut the transposon. after sequencing the self - ligated transpon, dna fragment flanking tn5 was obtained. the result showed 042bm - x1 contains a tn5 insertion in the gene smc00190, which function was unknown and was demonstrated to be related to salt tolerance by this study, and the gene was named as rst - 0x1

    通過ecori酶切突變株基因組，得到完整的tn5 （含有在大腸桿菌中起始復制的oriv ）及其側翼的序列片段，該片段自連后轉化大腸桿菌，以tn5兩端已知的序列設計引物進行測序。 blast的分析測序結果表明， 042bm - x1和042bm - x2中tn5分別定位在苜蓿中華根瘤菌1021染色體上smc02682和smc00419基因內部，本實驗證明它們和042bm耐鹽相關，命名為rst - 0x1和rst - 0x2基因。
  13. Dna sequences flanking tn5 - 1063 of 042bmr5 were amplified using inverse pcr, it was found that tn5 - 1063 was inserted into noeb gene

    對042bmr5突變株的基因組進行反向pcr ，擴增位於tn5 - 1063兩端的側翼序列。
  14. Infectious bursal disease virus has been a great concern for the poultry industry for a long time, particularly for the past decade when its " re - emergence " in variant or highly virulent forms. in this study, two sets of primers ( pta and pts, ibda and ibds ), flanking the hyper - variable region of vp2 gene, were designed to run a reverse transcription polymerase chain reaction ( primary rt - pcr ) and nested - pcr. both of these assays can amplify all of 12 reference strains which including pathotypes cibdv, vvibdv and vibdv, but not the 5 negative reference pathogens of chicken

    結果12個參考毒株均能擴增出約679bp的目的片段，而陰性對照的常見5種雞病病原：雞新城疫病毒（ ndv ） 、雞傳染性支氣管炎病毒（ ibv ） 、雞傳染性貧血病毒（ caiv ） 、大腸桿菌和多殺性巴氏桿菌均沒有擴增到任何片段；應用建立的技術對疑似ibd的34份臨床病料進行檢測，並同時在基礎rt - pcr擴增的片段內設計另一對引物進行nested - pcr 。
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