fluorescence excitation 中文意思是什麼

fluorescence excitation 解釋
激發熒光
  • fluorescence : n. 熒光(性)。
  • excitation : n. 1. 刺激,興奮,激發,鼓舞。2. 【物理學】激發;【電學】激勵、勵磁。3. 【植物;植物學】激感(現象)。
  1. Aliquots of cells were mixed 0. 15 % mg / ml fb - 28, and kept at 4c for 30min, fusion assays were conducted : fluorescence was measured immediately at regular time - points with fluorescence spectrophotometer with an excitation wave length of 560nm and emission wave length of 590nm. the percentages of membrane fusion was calculated. by monitoring fusion using the r18 assay, we found that the fluorescent brightener 28 influenced membrane fusion of virus and midgut epithelia cells

    此外,採用分子探針r18 (熒光標記物)標記病毒囊膜,體外分離中腸上皮細胞,將標記的病毒粒子與離體中腸上皮細胞混合后保溫,病毒吸附zh后,通過檢測熒光的變化來監測病毒粒子與上皮細胞的融合。
  2. Absorption, fluorescence emission and excitation spectra of five pigment - protein complexes were determined. photosynthetic electron transfer was measured from the dcip photoreduction. p700 concentration was assayed from the ferricyanide - oxdised minus ascorbate - reduced difference spectrum

    測定裙帶菜各色素蛋白復合物的吸收光譜、熒光發射光譜和熒光激發光譜,並進行了dcip的光還原測定和化學法的氧化還原差示光譜測定。
  3. Fluorescence power transfer function, three - dimensional point spread function ( 3d - psf ) and three - dimensional optical transfer function ( sd - otf ) for the various fluorescent wavelength of the two kinds of fluorescence confocal scanning microscopy are calculated in this paper by using fourier imaging theory. the results show that the fluorescent wavelength has influence on imaging property of confocal microscopy such as spatial cut - off frequency, resolution and 3d - otf. there is a different missing - cone in the 3 - d space of otf when the ratio of excitation wavelength to fluorescent wavelength decreases

    本文在sheppard和gumin等人的理論基礎上,利用fourier光學成像理論,討論了不同熒光波長對單光子和雙光子共焦顯微鏡成像特性的影響,導出了單光子和雙光子共焦顯微鏡的熒光功率傳輸函數、三維脈沖響應函數和三維光學傳遞函數,得到了它們在不同激發波長與熒光波長比值時具體的表達式,並且通過數值計算,得到了它們的曲線圖,結果表明:隨著激發波長與熒光波長比值的增加,焦斑的橫向分佈和縱向分佈變窄,橫向解析度和縱向解析度提高,系統的成像效果變好,當激發波長與熒光波長的比值下降到一定程度時,可以看到不同程度的失錐現象。
  4. Furthermore, the excitation spectrum for 730nm fluorescence shows that the 685nm light has high excitation efficiency

    葉綠體730nm熒光成份的激發光譜實驗結果表明: 685nm光對730nm熒光有較高的激發效率。
  5. The method for analyzing the excitation spectral structure of diatomic molecule with laser induced fluorescence

    激光誘導雙原子分子熒光激發譜結構的分析方法
  6. Green fluorescent protein has several good characters. under excitation of long uv light or blue light, it emits green fluorescence without requiring any exogenous substrates and cofactors. gfp gene expression can be used to monitor gene expression and protein localization in living cells and organisms. this is a development of revolutionary significance. the dna sequence of this gene can be re - engineered by mutagenesis and the gfp will get improved fluorescent properties. the applications of gfp will be wider and wider

    綠色熒光蛋白具有優良的特性,在藍光或長紫外光的激發下,不需要任何外源底物或內源輔助因子的參入就能發出綠色熒光.綠色熒光蛋白基因的表達可用來監控活細胞或生物體中基因表達和蛋白質的定位.這是一個革命性的進展.而且,對基因dna序列的改造有可能使綠色熒光蛋白的發光特性更加優良,從而其應用范圍會更加廣泛
  7. We also report the uv - visible optical absorption spectra of these colloids. to the metal plate prepared simultaneously, the measurement result of sem and afm of this plate is also reported and the roughness of the silver plate surface is about 60nm. both the colloids and the nano - scale metal surface can become the very perfect sers substrates. rhodamine b, a kind of strong fluorescence laser dye, with gold colloidal surface, has been studied by surface - enhanced raman spectroscopy ( sers ) with near - infrared ( nir ) excitation in the presence of the halide ions ( cf, br -, f )

    利用透射電子顯微鏡( tem ) ,對膠體粒子的尺寸大小及形態進行了觀測,表明這些膠體為粒徑介於5 35nm的納米體系,並對該膠體體系進行了紫外?可見吸收光譜的研究;在制備出金屬膠體的同時,經過激光照射后的金屬表面為納米量級的粗糙表面,對該表面進行了掃描電鏡( sem )及原子力顯微鏡( afm )的測量,結果顯示該表面的粗糙度平均為60nm 。
  8. As heavy oil has fairly high aromatic hydrocarbon compound characterized by a wide range of components, the application of three - dimensional fluorescence pattern composed of excitation wavelength, emission wavelength and fluorescence intensity can detect the composition, intensity and characteristics of aromatic hydrocarbon compound

    摘要根據重質油含有芳香烴化合物的組成范圍很寬、含量較高的特點,應用由激發波長、發射波長和熒光強度組成的三維熒光圖譜,可以檢測芳烴化合物組成、強度及其特徵。
  9. Abstract : polyphenylacetylenes were synthesized by rn ( nbd ) cl 2 and wcl6 / ph4sn catalyst systems in n2 at room temperature. polymers with high yield and high molecular weight were obtained. their structures were characterized by uv, ir, nmr and gpc, respectively. the fluorescence of the polymer solution with different concentration was investigated using variable excitation wavelength from uv to visible region. the relation between fluorescence and polymer structure was studied. the results showed that the molecular chain structure has great influence on fluorescence of the polymers. the emitting peak wavelength of fluorescence for the polymer of high stereoregularity is independent of solution concentration and excitation wavelength. the emitting peak wavelength of fluorescence for the polymer with disordered molecular chain arrangement varies with changing the solution concentration and excitation wavelength, and different emission sites in molecular chains and low emitting yield are shown. the measurement and analysis of fluorescence spectroscopy of polyphenylacetylenes may provide some useful information to judge the molecular chain arrangement in some conjugated polymers

    文摘:使用有機金屬銠、金屬鎢/四苯基錫催化劑體系聚合苯乙炔,分別獲得了高產率和高分子量的聚苯乙炔.使用uv , ir , nmr , gpc等分別對聚合物結構進行了表徵.採用不同波長的激發光對聚合物熒光性能進行研究,詳細分析了聚合物結構與熒光性能之間的關系.研究結果發現,不同聚合物結構對聚合物熒光性能產生很大影響,規整性高的聚合物,將有較高的發光量子效率;聚合物規整性差,將可能導致多個熒光發光結構點,其熒光強度降低.聚合物熒光光譜研究將對某些共軛聚合物結構的規整性分析提供一些有用的信息
  10. Changes in h2o2 generation in guard cells of vicia faba induced by aba were measured by using fluorescence probe, 8 - hydroxypyrene - l, 3, 6 - trisulfonic acid ( hpts ). examination of epidermis peel was performed using a laser scanning confocal microscope ( lscm ) and spectrofluorometer, set to an excitation light of 405 nm and an emission light of 512 ran

    以蠶豆葉片下表皮為材料,將熒光探針hpts導入蠶豆氣孔保衛細胞內,利用熒光光譜和激光共聚焦顯微鏡技術,檢測了aba誘導蠶豆氣孔關閉過程中h2o2的產生。
  11. To detect whether crosslinking reaction influence the fluorescence properties of the samples, oxygen - evolving psil core complexes were treated with five crosslinking agents at different concentration, then the chlorophyll fluorescence emission, excitation, and intrinsic fluorescence spectra of these samples were measured. the results showed that both chlorophyll fluorescence and intrinsic fluorescence of oxygen - evolving psil core complexes were altered by the crosslinking reaction. the maximum chlorophyll fluorescence band at 682 nm decreased and the intrinsic fluorescence emission intensity at 308 nm and 328 nm either increased or decreased

    結果表明:交聯處理對psll放氧核心復合物葉綠素熒光和內源熒光都有影響,引起682urn處葉綠素熒光強度的降低、 308urn或328urn處蛋白質內源熒光強度的增大或減小,並與處理時所用交聯劑的濃度、交聯劑的親疏水性和交聯臂長相關。
  12. Led light - induced fluorescence spectra of whole blood, erythrocyte and hemoglobin are obtained in the case of different concentration of blood cells solution with various excitation wavelengths. the intensity and peaks of the spectra from 430nm to 700nm depend strongly on the concentration of blood cells

    通過改變激發光波長和血細胞濃度,獲得了led光誘導全血、紅細胞和血紅蛋白產生的熒光光譜,其光譜覆蓋了430 700nm波段,且光譜強度和峰值位置均與溶液濃度有較強的依賴關系。
  13. The results indicated that the co - dopants of li +, na + and k + could promote the crystallinity of samples. however, the co - doping of mg2 + and al3 + drastically causes the structure disorder. ( 4 ) the fluorescence decay curves of 5do of samples were measured under the excitation of 266 nm laser

    比較發現,摻雜不僅可以調節納米材料的尺寸,還可以影響材料的結晶性,尤其是後者對發光性質和熒光動力學過程,如熒光強度、電荷遷移帶的位置、 ~ 5d _ 0的壽命等,產生強烈的影響。
  14. Mda ( 20mm ) reduced sharply the typical fluorescence of proteins ( excitation 280nm / emission 350nm ) and produced age pigment - like fluorescence with a strong emission peak at 460 nm excited at 395 nm when incubated at 37

    通過對脂質過氧化的次級產物? ?各類雙功能醛及其它單功能醛對不同生物溶液流變性及熒光特性的作用的對比研究。
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