free plasmid 中文意思是什麼
free plasmid
解釋
游離質粒-
Transfection of cell lines impaired for vzv infection with a plasmid expressing human ide resulted in increased entry and enhanced infection with cell - free and cell - associated virus
Vzv感染受抑制的細胞系通過質粒表達轉染人類ide蛋白后可增加游離病毒顆粒以及細胞內病毒引起的感染。 -
The morphological, physiological, biochemical and genetic diversities between bacillus thuringiensis wild - type strain ybt - 1463 and its plasmid - free mutant bmb171 was comparatively studied. it showed that the plasmid - free mutant strain bmb171 lost the ability to form the parasporal crystal, but there was on obvious diversities were observed on the sensitivity to 10 antibiotics, the utilization of 19 carbon sources and 12 nitrogen sources, as well as the growth properties between ybt - 1463 and bmb171, whereas the electro - transformation frequencies of bmb171 were much higher than those of ybt - 1463, respectively with 5 exogenous plasmids as the donor dnas
對出發菌株ybt - 1463和其無質粒突變株bmb171的部分形態、生理生化和遺傳學特性進行的比較研究的結果表明，突變株bmb171不形成伴胞晶體，但在個體形態與菌落特徵、對紅黴素等10種抗生素的敏感性、對葡萄糖等19種碳源和谷氨酸等12種氮源的利用能力及生長性能與出發菌株ybt - 1463無明顯差異。 -
After linerization, the recombinant plasmid was transformed into pic hia pastoris by electroporation, which then were cultured in md plate free of histidine, from which the positive colones were propagated
重組質粒線性化后，用電擊法將重組質粒轉化入巴氏畢赤酵母，在缺組氨酸的md板上篩選陽性菌落，然後用不同濃度的g418 ? ypd板篩選多拷貝插入單菌落。 -
The acrystalliferous and plasmid - free derivatives of bacillus thuringiensis were screened with ethidium bromide treatment, elevating growth temperature from 42 to 44 then to 46 by degrees, and treating it with 0. 05 % sodium docecyl sulphate ( sds ) as the plasmid - curing agent at 46
分別用溴化乙錠誘變處理、逐級升溫培養以及用sds處理等三種方法對蘇雲金芽胞桿菌無晶體（ cry ~ - ）和無質粒突變株進行了篩選研究。 -
The competition between plasmid - bearing organisms and plasmid - free organisms in the host was researched
摘要研究宿主體內帶質粒微生物和不帶質粒微生物的競爭關系。 -
The recombinant plasmids pbmb121l, pbmb9821l and pbmb986l were constructed after transferring bacillus thuringiensis icps gene crylaal, crylaclo and crylca from their original plasmid vectors to different plasmid vector. the relevant recombinant strains were obtained after introducing the 3 recombinant plasmids into bacillus thuringiensis plasmid - free mutant strain 8mb 171 by electroporation. the transformation and expression properties of 8mb 171 were studied
通過將蘇雲金芽胞桿菌殺蟲晶體蛋白基因cry1aal 、 cry1ac10和cry1ca轉移至不同質粒載體上，分別構建了重組質粒pbmb121l 、 pbmb9821l和pbmb986l ，並分別導入無質粒突變株bmb171 ，篩選得到攜帶相應icps基因的重組菌株。 -
The optimized parameters of high - frequency in bacillus thuringiensis plasmid - free mutant bmb171 by electroporation was studied. it showed that a highest electro - transformation frequency could be obtained, when sg solution was used as the buffer, and a 10. 0 kv / cm of field strength, one time of pulse as well as a growth phase of recipient cells at the exponential phase ( od650nm value was 0. 2 - 0. 3 ) were selected
對用電脈沖法轉化蘇雲金芽胞桿菌受體菌bmb171優化條件的研究結果表明，採用sg溶液作電脈沖緩沖液，用10 . 0kv cm的脈沖場強和1次電脈沖（ 4 . 6ms ） ，以及採用對數前期（ od _ （ 650 ）約0 . 2 0 . 3 ）收獲的受體菌，可以達到最高轉化頻率。
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