full open can 中文意思是什麼
full open can
解釋
全開蓋-
In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。 -
Pour one cup : the tea bag joins after go into the cup very hot open fire, soak 2 - 3 minutes, tremble to move the tea bag lightly. let full spillage of the tea flavor, can immediately drink. 1 - 2 packets of every time, everyday three time
沖泡一杯：茶袋入杯后，加入滾燙開火，浸泡二至三分鐘，輕輕抖動茶袋。讓茶味充分溢出，即可飲用 -
If we change our views on the requirement to the educational theories, regard them as the tools by which we can open our horizons, deepen our understanding, impel us to reflect ; if we take care our dream based on the reality, the teacher ' s personal practical theory becomes the possibility to make the question and the hypothesis, and criticize and improve their experience based on others " experiences and theories, then, the growth of the teacher becomes a process of full of reasonable and self - realizing life experience, instead of the process of obliged and forced and passive life experience. this is the indeed the possibility of the approach that the growth of the teacher will happen
如果轉變對教育理論的要求，將其視為開闊視野、深化認識、促進反思的工具而不是必須照辦的僵化的教條；在關照現實的基礎上胸懷理想，以教師個人實踐理論為根基，把它作為可以依靠、但是存有疑問的可能性和假設，並參照專業理論和他人經驗所提供的多種可能性逐步加以批判、改進，教師的成長才成為一個更加理性的、自為的生命歷程，而不是任由外部力量裹挾的、完全被動的過程，這才是既有立足點又有發展張力的實現教師可能發展的途徑。 -
Secondly, it sets forth the basic principle and constitutes of the full open cnc and so on, and focuses on down - level communication of hardware - function module by using wdm technology, the newest technology developed by microsoft co., and develops a wdm device driver for digital i / o module ( hy - 6140 digital input card ) in pcnc based on windows2000, which can rapidly and correctly reads the digital signal outside from the hy - 6140 digital input card through real testing
然後，闡述了全開放cnc基本原理和構成等，並把重點放在pcnc硬體功能模塊的底層通訊的開發上，採用微軟推出最新一代的wdm技術，在windows2000平臺上為pcnc中的數字i / o模塊（ hy - 6140數字輸入卡）開發了一個wdm設備驅動程序，通過實際測試能夠實時準確地讀取外部的數字信號。 -
Each county ( city, area ) party committee, government must improve knowledge further, execute promotive assist qualification strictly to affirm hair of administrative power of authority, capital, capital to put authority and social superintendence " 4 authority " detached, the fairness that ensures promotive assist works, justice and open, make reward assist gold can seasonable, full specified amount, extend surely in assist object hand
各縣（市、區）黨委、政府一定要進一步提高熟悉，嚴格實行獎勵扶助資格確認權、資金治理權、資金發放權和社會監督權「四權」的分離，確保獎勵扶助工作的公平、公正和公開，使獎勵扶助金能夠及時、足額、安全地發放到扶助對象手中。 -
You can show your endless vigor in the spacious open - air swimming pool or keep fit in the fitting room with the outstanding facilities, or even give full play to your skills in the tennis court
您可在寬敞的室外游泳池隨心暢泳，或到器材完備先進的健身室鍛練身體，更可到網球場一展身手。 -
And it can help us to open up the utilization scope of frequency changer. the main content have been studied in this paper. for example, the energy saving effect of the frequency changer utilization in tube - well irrigation, spray irrigation and drip irrigation, the influence of the frequency changer to the degree of consistency in tube - well irrigation, spray irrigation and drip irrigation, and the full - scale test of the frequency changer used in tube - well irrigation
本項目在國內首次提出了變頻調速裝置在管灌中使水泵進行「恆量變壓」變頻運行的設想，並首次對變頻調速裝置在農業節水灌溉中的應用進行了實地試驗研究，該研究成果對變頻調速裝置在管灌、微噴及大棚滴灌中的應用具重要的指導意義，對變頻調速裝置的設計研究及其應用范圍的拓展等具有一定的理論意義和很大的實用意義。 -
It can break time and apace limit, broad in scale, low cost, has an advantage in optimization and sharing the educational resources. the forms of its running school are open, flexible, rich in teaching resources and diversified forms. it can completely take an important part in the teachers continuing education, meet the needs of the teachers continuing education with full play, promote them to change the educational sense, make convienent for teachers advanced studies and teaching research in the lake - developing areas
方興未艾的現代遠程教育，其教育技術手段先進，能突破時空的限制，規模大、教育成本低，具有優化和共享教育資源的優勢，辦學形式開放、靈活，完全能夠在欠發達地區教師繼續教育中發揮重大的作用：最大限度地滿足欠發達地區教師繼續教育的需求，促進教師教育觀念的轉變，節省開支，緩解工學矛盾，教學資源豐富、前沿、格式多樣，方便教師進修和開展教學研究。 -
Powerful : only need to open the switch, can realize full - automatic control
功能強大：只需打開電源開關，就可以實現全自動化的控制 -
Along with the course of reform and open, wto joining of china, and development at full speed of information technology, the enterprise more and more is hard to suit with " customer " and " competition " and " change " for the modern enterprise of characteristic manages the environment. it is leading no matter the company adopts the cost or the strategy to carry out the difference management all will be faced with the bigger difficulty. only by way of raising the management standard, the challenge of new times can be met to efficiency and the quick reaction capability to raise the company
隨著改革開放的進程，中國的加入wto和信息技術的飛速發展，企業越來越難以適應以「顧客（ customer ） 、競爭（ competition ） 、變化（ change ） 」為特徵的現代企業經營環境，公司無論是採取成本領先還是實行差異化經營的戰略都將面臨著更大的困難，只有通過提高管理水平，提高公司的效率和快速反應能力才能迎接新時代的挑戰。 -
For the full open doors report, you can find a link to the iie website at voa special english report
關于全面開放報道，你可以在美國之音特別英語報道的iie網站里找到相關連接。
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