full-length rna 中文意思是什麼
full-length rna
解釋
全長rna-
In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。 -
Linearized full - length cdna was used as template then genomic rna of csfv was in vitro transcriped by t7 rna polymerase
以線性化的全長cdna為模板，體外轉錄得到了csfv基因組rna 。 -
Detailly, it was ( l ) to isolate and construct high efficiency expression vector of rice starch branching enzyme gene sbe2b and ( 2 ) to establish a high - effecient rice transformation system. total rna was extracted from maize endosperm 15dap ( days after pollination ). and cdna of sbe2b was obtained through rt - pcr. sequencing result showed that the full length cdna was 2. 4kbp, coding for 800 amino acids, with estimated mw 93kd
本研究從授粉15天左右的玉米胚乳中提取了總rna ，採用rt - pcr方法，克隆了玉米胚乳的澱粉分支酶基因sbe2b ，測序結果表明，玉米胚乳sbe2b的cdna全長為2 . 4kb ，編碼800個氨基酸，推測的氨基酸的分子量為93000d ，該序列與genbank中登錄號為af072725的玉米sbe2b的cdna序列有98的同源性，與水稻、大麥、小麥等禾本科植物的有關澱粉分支酶的dna序列都有極高的同源性。 -
To device a primer pairs and amplify the full length pstvd by rt - pcr, the positive rna extraction from tuber sample was used as template. the product of rt - pcr was purified and connected to the plasmid pmd 18 - t vector
Cdna核酸斑點雜交反應（ nash ）檢測pstvd方法準確、靈敏度高，一次檢測樣品數量多，且對于異地樣品檢測非常方便，是以往其它檢測方法的有效補充。 -
Successfully cloned and constructed infectious full - length cdna of attenuated lapinized csfv chinese - strain ( derived from spleen ) could make us get pure rna virus genome of csfv c - strain, and further study and utilize mutation, deletion, insertion and substitution of csfv gene on dna molecular level
中國豬瘟兔化弱毒（脾淋毒）全長感染性cdna的克隆和構建，可以使我們得到純粹的csfvc -株rna病毒基因組，在dna水平上研究和利用csfv的基因突變、缺失、插入和替換。 -
[ methods ] by using the overall rna of our previously cultured human melanoma cell line ( a375 ), full length fasl gene is detected by rt - pcr. using the cdna as template, . the extracellular domain of fasl ( fasl - ecd, 127 - 278aa ) is amplified by pcr. the pcr products are directly cloned into t vector pmd - 18t
L方法採用新鮮人黑色素瘤細胞（ a375 ） ，抽提該細胞的總rna ，進行rt一pcr反應分析a375內fasl全長編碼基因的轉錄表達，以a375細胞cdna為模板，用pcr產物直接克隆法擴增人fasl一ecd （人fasl胞外區）的編碼基因，即127一278位氨基酸殘基，而後將pcr產物直接克隆于pmd一1st載體中，獲得重組質粒pmd一t - fasl一ecd ，進行dna測序。
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