fused protein 中文意思是什麼

fused protein 解釋
融合蛋白質
  • fused : adj. 1. 熔融的，熔凝的。2. 融合的，合併的。
  • protein : n. 【化學】朊，蛋白（質）。
  1. Whole mount in situ hybridization showed that amphihmgb transcripts were present ubiquitously in the early embryos from zygote to neurula, but they decreased gradually in mesoderm and endoderm and neural tube with development, and became restricted to epidermal cells in the hatched larva. it appear s that hmgb plays a role in the mitosis and differentiation of embryonic cells. another amphioxus cdna, amphiubf80, encoding ubiquitin / ribosomal fused protein s27a, was isolated from the gut cdna library of b. belcheri tsingtauense

    S004cdna序列編碼一個156個氨基酸殘基組成的蛋白質，與人的泛素蛋白ubf80具有較高的氨基酸序列同源性（ 145 / 156同源） ，且該蛋白具有泛素蛋白ubf80所共有的兩個結構域，即由72個氨基酸組成的泛素介導的蛋白降解結構域和由48個氨基酸組成的核糖體蛋白s27a結構域。
  2. Healthy rabbits were inoculated each with vaccine of the recombinant fused pili - el - 2 protein at 250ul per dose to potentiate the immunogenicity. rabbits inoculated two times at one week interval

    擴增此質粒，回收bamh切出的2 . 5kb的基因片段，與pme290 （ bamh酶切後去磷酸化）載體連接。
  3. The blood of each rabbit was collected at days 0, 7, 14, 2l, 30. the antibody titers were evaluated by k - agglutination test. the results showed that lower agglutination titers was observed at day 7 and up to higher levels over 8000at day 21 induced by the vaccine of recombinant fused protein

    將融合基因工程菌株在營養肉湯中培養，離心後上清用0 . 1mmgcl _ 2沉澱，離心，提取表達產物。經sds - page電泳證明表達產物為纖毛蛋白與il - 2的融合蛋白。
  4. Phage display describes a selection technique in which a peptide or protein is expressed as a fussion with a coat protein of bacteriophage, resulting in display of the fused protein on the surface of the virion, while the dna encoding the fussion resides within the virion

    自1985年gpsmith首次提出噬菌體展示技術以來，隨著生物技術的發展，噬菌體隨機肽庫已成為研究分子間相互作用的有力工具，特別是在抗原表位研究方面。
  5. To search proteins that associate with the mouse mint protein and regulate notch signaling in nuclei, and to study the function and mechanism of mint - mediated transcription repression, yeast two - hybrid assay was used to screen proteins that interact with a fragment of mint ( f5, amino acids 2226 - 2959 ). from 4x106 yeast clones transformed with the bait plasmid and a cdna library of 9 dpc mouse embryo, fifty - one were positive for nutritional screening and p - galactosidase assay. restriction digestion identified 10 independent positive clones and these were analyzed by dna sequencing these clones represent 3 correctly fused cdna fragments, which are mint, alpha a - crystallin - binding protein i ( alphaa - crybpl ), and nuclear receptor co - repressor 1 ( n - corl ), respectively

    鑒于mwt基因編碼區較長，共有10799個堿基，故此我們將mint分為六段，分別命名為fi一f6 ，本研究以其中的f5 （ 222e959 ）和f6 （ 296s576 ）片段為研究對象，將h者分別插入pgb盯7載體中，結果顯示： mintfs可與核受體輔助抑制因子1階cori ） ， o晶狀體蛋白結合蛋白1hlphatcrybp入及mw加互作用，而f6可與igm的重鏈恆定區、泛素結合酶2l6和一個未知功能的新的蛋白基因進行結合。
  6. Meq protein, highly expressed in the insect cell line sf9 by the baculovirus vector was immunized into balb / c mice and the immunized spleen cells were collected and fused with the tumor cell line sp2 / 0 via peg - 1000 in vitro. the hybridoma cells were cloned and screened for the ability of anti - meq mcab secretion by fa with the mdv ga infected chicken embryo fibroblast ( cef )

    利用通過桿狀病毒載體在昆蟲細胞系sfg上高度表達的meq蛋白產物免疫balb / c小鼠，然後收獲其免疫脾細胞並與腫瘤細胞系spz / 0通過peg于體外融合;獲得的雜交瘤細胞被克隆並通過與mdv感染的雞胚成纖維細胞（ cef ）做免疫熒光試驗（ fa ） ，進行其分泌抗meq單克隆抗體（ mcab ）能力的篩選。
  7. We fused the gfp into the c - terminal region as well as n - terminal region of emt - 1 protein. the result showed that the localization of the protein encoded by the full length emt - 1 cdna was in endoplastic reticulum ( er ). extracellular region, transmembrane and intracellular region showed the similar cellular localization

    我們用綠色熒光蛋白融合於emt l全長及其不同截斷形式的梭基和氨基端，瞬時轉染cos 7細胞，通過熒光顯微鏡觀察，發現emt l編碼的蛋白呈現內質網定位的特點。
  8. The constructed plasmid containing gst - src fused gene gave a high level of expression of the fused protein

    用iptg誘導表達蛋白的產生，純化后獲得大量的目的蛋白。
  9. A conservative motif, recognized by proteinases of potato virus y, was inserted between nib and ppiv, which will release functional ppiv from the fused protein after infection by potato virus y. then, plant expression vector pnpa was constructed by ligating the fusing gene and pbi121, which is knocked out gus gene

    以馬鈴薯y病毒的復制酶（ nib ）基因為模板，通過聚合酶鏈式反應獲得nib基因，並在nib基因末端保留了在病毒基因組中nib與外殼蛋白（ cp ）基因相銜接的保守序列。
  10. The transmembrane activity of the ptd of tat protein fused at the carboxyl - terminus of gfp in vivo

    端融合在活體體內的跨膜遞送作用
  11. The ha 122 protein, when fused to gfp was observed in the nuclei of h. armigera cells, but only in conjunction with wild type hasnpv infection

    將ha122與綠色熒光蛋白（ gfp ）基因融合，在昆蟲細胞中瞬時表達。當有病毒感染同時發生時， gfp信號定位在核內。
  12. To allow secretion of the crylac protein into the intercellular space, potato proteinase inhibitor ii ( pinll ) signal peptide sequence was n - terminally fused to the crylac coding region to construct plasmid p3301ubisigac

    運用pcr技術克隆了馬鈴薯蛋白酶抑制劑基因的信號肽序列，並將其分別連到crylac 、 gfp基因的5 』端，構建植物轉化載體p3301ubisigac和p3301ubisiggfp 。
  13. To express hau3r gene as fusion protein, it was cloned into pgex - kg and fused with gst, which is controlled by promoter ptac

    將hau3 ~ r基因與融合基因表達載體pgex - kg的ptac啟動子控制下的gst融合，獲得重組質粒phz2083 。
  14. It is the foundation of therapy of hepatitis b. the gene of scfv was cloned into the expression vector pet32a ( + ) and subsequently expressed in escherichia coll. the expressed scfv fused with the 109 aa trx - tag thioredoxin protein was above 30 % total cell protein in the shaking flask cultures

    融合硫氧還蛋白的單鏈抗體在大腸桿菌bl21 （ de3 ）中以包涵體的形式得到高效表達，單鏈抗體表達量達佔30的總蛋白以上。
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