fusion bodies 中文意思是什麼

fusion bodies 解釋
融合體
  • fusion : n. 1. 熔解,熔化;【物理學】(核)聚變,合成。2. 〈美國〉融合;(政黨等的)合併,聯合。
  • bodies : 本斯瓊斯氏體
  1. The inclusion bodies of recombinant protein were purified with washing buffer consisting of various urea ( 2mol / l and 4mol / l ) for several times, and then dissolve the fusion protein in the denature buffer using 8mol / l urea as denaturant

    用含有2mol l和4mol l尿素的包涵體洗滌液洗滌包涵體,在37條件下,洗去了大部分菌體蛋白及其它核酸物質。用8mol l尿素作為變性劑溶解包涵體,包涵體在8mol l尿素中的溶解性非常好。
  2. External fertilization occurs when gametes are expelled into a watery substrate from the parental bodies before fusion ; it is typical of sporebearing plants

    當配子在融合之前從親本體中排出到有水介質時發生體外受精,這是孢子植物的特徵。
  3. Its content was about 9. 8 % among total cell protein by gene genius bio imaging system. the fusion proteins were found largely in an insoluble inclusion bodies. the purified fusion proteins was obtained by his6 technique used to immunize rabbits to obtain polyclonal antiserum with titer of 1 * 105

    經工ptg誘導,重組質粒在點co力『 blzi中表達出了c端融合了6xhis的融合蛋白,過量表達的蛋白主要以不溶性蛋白形式存在,其表達量占菌體總蛋白的9 . 8 % 。
  4. Telamon : greek for support, what ' s telamon main function in between vertebral bodies while the fusion is getting done

    希臘語是"支持"的意思, telamon的主要功能是在融合完成後在椎體間起支持的作用。
  5. Bonification and centrifu - gation were recommended for the lysis of collected cells and the supernatant and precipitation were collected respectively. as to the plenty of include bodies in the precipitation, denationalization, detergence, purification and dissolution, last regeneration were recommended to acquire great deal of expressed gst fusion proteins

    Coltblzi生產融合蛋白,重組后的dna序列包括一個pgex質粒,依照pgex質粒的融合蛋白的表達是在tao啟動子的控制之下,而枯啟動子可由乳糖的類似物i剛來誘導它表達。
  6. Get the inclusion bodies 2 ) western blot analysis of fusion protein expression ( 1 ) electrophoresis ( 2 ) transfer proteins from gel to membrane ( 3 ) blocking ( 4 ) incubation with primary antibody ( 5 ) enzyme conjugate incubation ( 6 ) substrate incubation. 3 ) gst detection module with cdnb enzymatic assay 3 purification of gst fusion proteins 1 the denaturalization of inclusion bodies. 2 purification using glutathione sepharose 4b column wash matrix with 1 pbs, prepare a 50 % slurry for batch purification method, pack column with matrix slurry

    三、 gst一hbrp重組蛋白的純化1 .超聲破碎細胞,離心,上清和沉澱進行sds一page電泳分析2 .樣品處理提取包涵體,變性后,加人用pbs平衡過的以utal腸onesepb抓脫4b ,室溫下孵育3 .以utadtionesepharose4b柱純化以utad雲onesepharose4b柱的準備根據毛山lel ,決定純化所需的gll衛ta1如oneseph娜se4b的柱床體積,用預冷的1xpbs清洗cldtathionese戶, se4b ,得到50 %的基質
  7. The results show that the optimal conditions of the expression of bl21 ( de3 ) - ptxbl - hng and bl21 ( de3 ) - ptxb1 - m - insuiin are : bl medium, 37, 200rpm for 4. 75h, then 1ptg was added to the medium to 0. 01mmol / l, induce for another 5h. 3. hng fusion protein and m - insulin fusion protein are expressed as inclusion bodies in e. coli

    工程菌表達條件的研究山西醫科大學2003屆碩士學位論文表達鑿l株blzi ( de3 )一ptxbi一hng及bl21 ( de3 )一ptxbi一介insulin均在bl培養基, 37oc 、 zoorpm搖床培養4小時45分時進入對數生民中後期,此時加入終濃度0 . olmlnol / l的i屍tg開始誘導,再持續培養5小時可達到最人表達量。
  8. The percent of the fusion protein in that of the bacterium bodies is 23. 6 %, and the produced zz : g3 fusion protein present a single band after purified by affinity igg - sepharose. the systematic comparison and analysis of expression of pheromone 3 of euplotes in

    重組菌在0 . 4mmol / liptg誘導下進行分泌融合表達,融合蛋白的表達量約占菌體總蛋白的23 . 6 ,表達產物zz : g3經igg - sepharose親和純化后基本為單一組分。
  9. The conventional spinal fusion procedure, in which a bone graft ( harvested from patient s own pelvic bone ) is placed between vertebrae, aims to unite the adjacent vertebral bodies after several months of healing. fusion has been the traditional operation to alleviate neck and arm pain, a concept which is similar to that used in the welding in industry

    醫生會從患者身上(一般為髖骨的部位)取出骨骼作移植物,填補受損的椎間盤,待數個月後骨骼細胞生長后,兩節脊椎便會自動融合,病人的神經或脊髓不再受壓,紓緩患者頸部及手臂痛楚。
  10. We find that the fusion proteins are inclusion bodies when the bacteria lysised by the lysozyme. the product of pet - 32a ( + ) - igf - i was analyzed by western blotting, which confirmed that the hybrid protein was expressed as expected

    菌體採用溶菌酶裂解,融合蛋白以包涵體形式存在;對higf -融合蛋白進行了westernblot分析,確認所表達蛋白為目的蛋白。
  11. The recombinant fusion protein was highly expressed in e. coli bl21 induced by immol / l iptg in the form of inclusion bodies. the molecular weights of gst - h a1 is 62kd

    將構建好的融合表達載體,在1mmol l的iptg誘導下,在大腸桿菌bl21中得到大量表達,經過4h表達量既達到高峰。
  12. After dissolving inclusion bodies, renaturing inclusion bodies and further purification by immobilized metal ion affinity chromatography ( imac ), the fusion protein trx - scfv of electrophoretic purity was obtained, they still possessed antigen - binding activity

    經變性復性,用固相金屬離子親和色譜法一步分離純化了具有功能的單鏈抗體,在一升的發酵液中得到78mg的重組蛋白。
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