fusion of cells 中文意思是什麼

fusion of cells 解釋
細胞融合
  • fusion : n. 1. 熔解，熔化；【物理學】（核）聚變，合成。2. 〈美國〉融合；（政黨等的）合併，聯合。
  • of : OF ＝Old French 古法語。
  • cells : cells]上皮細胞
  1. After this aggregation, paramyxoviruses effect fusion of the agglutinated cells.

    在這種聚合之後，副粘液病毒能使凝集的細胞發生融合。
  2. Aliquots of cells were mixed 0. 15 % mg / ml fb - 28, and kept at 4c for 30min, fusion assays were conducted : fluorescence was measured immediately at regular time - points with fluorescence spectrophotometer with an excitation wave length of 560nm and emission wave length of 590nm. the percentages of membrane fusion was calculated. by monitoring fusion using the r18 assay, we found that the fluorescent brightener 28 influenced membrane fusion of virus and midgut epithelia cells

    此外，採用分子探針r18 （熒光標記物）標記病毒囊膜，體外分離中腸上皮細胞，將標記的病毒粒子與離體中腸上皮細胞混合后保溫，病毒吸附zh后，通過檢測熒光的變化來監測病毒粒子與上皮細胞的融合。
  3. E. coli xl1 - blue cells were tansformed by psurfpga and phages were rescued by m13ko7 helper phage particles. results showed that the heterodimeric enzyme was expressed as a fusion protein that matures to an active biocatalyst connected to the coat protein of phage fd

    以構建的噬菌粒psurfpga轉化具有琥珀突變的大腸桿菌xl1 - blue ，以輔助噬菌體m13k07超感染，進行青霉素g酰化酶基因的表達和在噬菌體表面的展示。
  4. In particular, we will focus on the ability of cell fusion that is caused by viruses to induce chromosomal instability, a common affliction of cancer cells that has been thought to underlie the malignant properties of cancerous tumours

    特別是，我們將聚焦于細胞的融合能力，該能力由病毒引起來誘導染色體不穩定性，而不穩定性則是癌癥細胞中常見的異常，其被認為癌癥惡性特徵的基礎。
  5. To investigate the consequence of this interaction, aes - rfp fusion protein expression vector was constructed and co - transfected into nih 3t3 cells with tle1 - gfp fusion protein expression vector. confocal microscopy observation showed that aes could interact with tle1 at the cytomembrane region. moreover, this interaction inhibited the concentration of tle1 into nucleus

    在構建了紅色熒光蛋白aes表達載體后，將其與tle綠色熒尤蛋白載體共轉染細胞，共聚焦顯微鏡觀察發現這兩種分子在胞漿中有共存現象，而且aes的表達可抑制tlei向胞核內的聚積。
  6. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  7. Different from mammals, the early embryos of fish can not be preserved for the long period at the very low temperature ( - 196 ). therefore, three methods were usually applied to cryogenic preservation of the fine and rare species of fish : 1 ) perserving fish spermatozoon in cryogenic condition. researchers have had systematically studied on this technique for many years, and this technique has been utilized in application and made a lot of effects ; 2 ) combining with the techniques of cell engineering ( nuclear transplantation and electric fusion etc. ), and through the process of culturing histiocyte of fish, cryopreservation and re - culture after thawing, carrying out somatic cell breeding of fish. the past studies showed that the nucleolus of somatic cells of fish have totipotency

    多年來，國內外學者對各種魚類精液的冷凍保存進行了大量的系統研究，目前這項技術已達到實用水平，並日益發揮作用；二是對魚類培養的組織細胞冷凍保存，通過魚類細胞的培養、超低溫凍存、解凍后再培養過程，結合細胞工程技術（如核移植、電融合等）進行體細胞育種；大量的研究結果表明魚類體細胞核具有發育的全能性，隨著細胞培養技術、細胞工程技術日益發展成熟，完全具備實現魚類物種種質長期保存的理論基礎和技術條件。
  8. In order to get further evidence of the localization and distribution of emt - 1 in cells, we have prepared emt - 1 his6 - fusion protein and intend to abtain emt - 1 antibody for immuno - histochemistry assay

    為了進一步證實emt l在細胞內的定位及組織分佈，擬制備抗emt l抗體，進行免疫組化驗證。
  9. The follicle cells taked part in the synthesis of yolk precursor, and the yolk precursor were transported into oocyte by pinocytosis and fusion. after follicle cells secreting egg shells, oogenesis completed

    在幫助卵母細胞沉積完卵黃后，濾泡細胞還分泌卵殼蛋白，形成卵殼之後退化消失
  10. Somatic hybrid cells were obtained between ethionine resistant cell line of astragalus adsurgens pall, and agrobacterium / " / z / zogerae. s ' - transformed cell line of medicago saliva l. using protoplast fusion by peg method

    通過peg誘導原生質體融合和雜種細胞的篩選培養，首次得到沙打旺（ + ）紫花苜蓿的屬間體細胞雜種。
  11. Expression amount of rhbsag in the former was increased 60 % and 80 % respectively than the latter. antigenicity of pres2 of fusion protein was increased 8 times and 10 times in bmn cells and in pupa hemolymph respectively than nonfusion hbsag

    Bmpak - hbmp表達的融合m蛋白（ pres2 + s ）中， pres2抗原性顯著提高，與bmpak - hbm表達的非融合蛋白相比，融合蛋白pres2抗原性在細胞中約提高了8倍，蛹中約提高了1o倍。
  12. Bonification and centrifu - gation were recommended for the lysis of collected cells and the supernatant and precipitation were collected respectively. as to the plenty of include bodies in the precipitation, denationalization, detergence, purification and dissolution, last regeneration were recommended to acquire great deal of expressed gst fusion proteins

    Coltblzi生產融合蛋白，重組后的dna序列包括一個pgex質粒，依照pgex質粒的融合蛋白的表達是在tao啟動子的控制之下，而枯啟動子可由乳糖的類似物i剛來誘導它表達。
  13. Construction of p21 egfp fusion gene expression vector and its expression in hrpe cells

    15細胞乙型肝炎病毒復制的實驗研究
  14. The cytotoxicities of rta and the fusion toxin rta - yqrl were measured by the mtt assay in hela, skov - 3, and wish cells following fluid - phase endocytosis. [ methods ] 1

    因為rta可與f3ga發生特異性結合，我們用bule一sepharose一6b柱對rta和rta一yqrl蛋白分別進行親和層析純化。
  15. Rab gtpases are involved in cell differentiation, migration, endocytosis and exocytosis, cytosolic proteins transport and regulation of the transport and fusion of vesicle. in neuron cells, rab gtpases play a crucial role in synaptic vesicle transmission and transmitter release

    Rab蛋白主要通過調節囊泡（ vesicle ）的出芽（ budding ） 、運輸、錨定和融合，參與細胞的分化發育、細胞的內吞和外排、胞內蛋白的運輸、交換和定位以及神經遞質的運輸和釋放。
  16. To observe tsarg2 fusion protein expression in mammalian cell, pegfp - nl / tsarg2 fusion plasmid was constructed and transiently introduced into cos7 cell by liposome transfection. under the fluorescence microscope, the green fluorescence produced by pegfp - nl / rsarg2 was detected on the nucleus of cos7 cells after 24 hours post transfection, while the fluorescence produced by pegfp - nl was detected through the cells

    同時將21大、 35天、 49大、 280天小鼠睪丸進行組織石蠟切片，結果表明小鼠翠丸在第21天可見精子細胞，到第35天，精子生成的最後階段開始發生，部分牛精小管中可見少量成熟的精於。
  17. Hfea deferred a decision on other types of human - animal embryos, such as what are known as " true hybrids " ? created by the fusion of a human sperm and an animal egg ? and so - called " human chimeras, " where human cells are injected into animal embryos

    Hfea延緩了針對另外一種類型的人-動物胚胎的決定，例如已為我們熟悉的「真雜交體」 ? ?通過融合人精子和動物卵細胞製造的雜交體? ?因此被成為「人嵌合體」 ：人細胞被注射到動物胚胎中。
  18. To circumvent these deficits, novel antigen - delivery systems utilizing cytokine gene - modified tumor cells and dc or fusion of dc with tumor cells have resulted in induction of antitumor immunity. however, this u approach is difficult in some cases ( for example in breast cancer ) because only rarely has it been possible to isolate enough viable tumor cells from an individual to prepare the vaccine

    為了克服上述缺陷，有學者採用滅活的腫瘤細胞、腫瘤抗原提取物（包括腫瘤細胞裂解物、 it na和洗脫肽等）沖擊致敏dc或將腫瘤細胞與dc融合后再回輸體內以激發機體的抗腫瘤免疫應答，也取得了較好的療效。
  19. Methods employed to prepare vaccine include rumor cells genetically modified with cytokines, costimulatory molecules and tumor antigenic peptides, dendritic cells ( dc ) primed with tumor antigens in vitro or genetically modified with tumor antigens, or fusion of tumor cells with antigen presenting cells ( apc )

    現有的比較受到關注的制備腫瘤疫苗的方法有各種細胞因子、共刺激分子、腫瘤抗原肽等基因修飾的腫瘤細胞疫苗；體外抗原致敏的或腫瘤抗原肽基因修飾的樹突狀細胞疫苗；腫瘤細胞與抗原遞呈細胞融合疫苗等。
  20. Hybridomas secreting monoclonal antibodies ( mcabs ) specific for clostridium perfringens type a enterotoxin ( cpe ) were produced by fusion of nso myelomas cells with spleen cells from balb / c mice immunized with purified cpe. wells containing hybridomas secreting immunoglobulin g ( igg ) antibodies against cpe were specifically identified by an indirect enzyme - linked immunosorbent assay ( elisa ), and two strains of elisa - positive hybridomas were selected and cloned forth by limited dilution

    該純化cpe對小白鼠的半數致死量為2 . 5ug /只；該cpe豚鼠皮膚藍斑單位為2500 4000 ；引起紐西蘭白兔小腸積液的最小毒素量為25ug 。將純化的cpe ，在含0 . 4福爾馬林的的pb液中透析制備類毒素。
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