gel chromatography 中文意思是什麼

gel chromatography 解釋
凝膠層析法
  • gel : n. 凍膠,凝膠(體);定型發膠。vi. (-ll-) 成凍膠,膠化。
  • chromatography : n. 【化學】層析,色層(分離)法。
  1. It was very slow at 5 but become higher above 25. the enzyme from abomasums used in cheese production was rough extracts containing chymosin and pepsin. through the gel filtration chromatography and ion exchange chromatogra

    羔羊胃蛋白酶最適凝乳溫度為45 』 c ;在45處理30inin ,酶活性開始下降, 60c處理30lliln ,酶活性完全喪失;酶的最適ph為1
  2. Two protein peaks can be obtained by bio - gel p - 6 chromatography and both peaks have antimicrobial activity. so the bacteriocin is consisted of two proteins with different mw. only one protein with larger mw can be detected through tricine - sds - page, and its mw is about 8, 570da

    採用30硫酸銨就能完全把發酵液中的細菌素全部沉澱,通過生物膠bio - gelp - 6層析發現細菌素被分離出兩條抗菌蛋白峰,這表明r21 - 4產生的細菌素是由兩種不同分子量的蛋白質組成的,通過tricine - sds - page檢測,只能檢測到一條分子量相對較大的細菌素,分子量在8 , 570da左右。
  3. The metabolites eliciting inhibition to foam cell formation process of macrophage produced by endophyte hccb00017 were studied. several products were isolated through solvent extraction, and silica gel chromatography et al. one compound, hccb00017 - a, showed cytotoxicity ; the other two, hccb00017 - c and hccb00017 - e, showed inhibitory activity against foam cell formation process of macrophage

    對具有巨噬細胞泡沫化抑制活性的植物內生菌hccb00017的代謝產物進行研究,應用溶媒萃取、硅膠柱分離等方法,從其發酵液中分離出具有細胞毒性的活性物質hccb00017 - a ,以及具有巨噬細胞泡沫化抑制活性的組分hccb00017 - c和hccb00017 - e 。
  4. The separation of the formica rufa linnaeus samples by pel filtration chromatography. in this part, the author has primarily separated the formica rufa linnaeus samples by glucosan gel g - 50 filtration chromatography. the principle is the difference of the molecular weight of different components

    紅褐林蟻樣品的凝膠過濾層析分離本部分旨在通過葡聚糖凝膠g - 50過濾層析初步分離紅褐林蟻樣品,根據分子量大小不同而達到分離目的。
  5. Strain bl21, and gene expression was induced by iptg. the target proteins were directed into the periplasmic space by the staphylococcal protein a signal sequence preceding the rgd - hirudin gene. using ion exchange chromatography and gel filtration chromatography, the chimera proteins were purified, and both of them showed a single band in tricine - sds - page. the results of activity analysis suggested that these two chimera proteins not only have antithrombin activities, but gain platelet aggregation inhibitory activities as well

    通過離子交換層析和凝膠過濾層分別對兩種嵌合體蛋白進行純化,純化產物在tricine - sds - page中都顯示為單一條帶。活性分析結果表明兩種嵌合體蛋白在保留水蛭素抗凝血酶活力的同時,還呈現抗血小板聚集活性。
  6. 6 - phosphogluconate dehydrogenase ( 6 - pgadase, ec 1. 1. 1. 44 ) was isolated by homogenate, ammunium sulfate fractionation, deae - sepharose chromatography, blue - sepharose affinity chromatography and gel filtration with sephadex g - 200 from bacillus subtilis, and some properties of the enzyme had been studied. a 113. 8 - fold purification was obtained with a 8. 2 % yield. the purified enzyme moved as a single electrophoretic band in page

    將枯草芽孢桿菌超聲波破壁后的粗提取物進行分段鹽析、 deae - sepharose陰離子交換柱層析, blue - sepharosecl - 6b特異結合柱層析和sephadexg - 200凝膠過濾等純化步驟,得到聚丙烯酰胺凝膠電泳為單一蛋白區帶,比活為1 . 46u mg的酶制劑。
  7. We got 4 parts from the extraction of astragalus variabilis bunge through systemic research ways of phytochemistry, which respectively are the part of petroleum ether, the part of petroleum ether, the part of etoa and the part of n - buoh the part of n - buoh are isolated by the successively methods of silica - gel column chromatography, tlc and sublimation

    摘要採用植物化學成分系統研究的方法,從變異黃芪醇提總浸膏中分段萃取得到石油醚提取部分、氯仿提取部分、乙酸乙酯提取部分和正丁醇提取部分。
  8. Study on analysis of trace ni in samples for resid gel permeation chromatography

    重油凝膠色譜制備樣品中痕量鎳的分析研究
  9. The xerocomus spadiceus lectin, xsl, was isolated from extracts of fruiting bodies of the mushroom xerocomus spadiceus using a procedure that involved ( nh4 ) 2so4 precipitation, anion exchange chromatography on deae - cellulose, affinity chromatography on affi - gel blue gel, cation exchange chromatography on cm - cellulose, and gel filtration by fast protein liquid chromatography on superdex 75

    從磚紅絨蓋牛肝菌( xerocomusspadiceus )子實體粗提物中,經過deae -纖維素陰離子交換層析、 affi - gelbluegel親和層析、 cm -纖維素陽離子交換層析和superdex75fplc凝膠過濾,純化了磚紅絨蓋牛肝菌凝集素。
  10. The powder was then applied to a silica gel column chromatography charged with 80 % acetone in water and eluted with the same solvent. after concentrating and drying, relatively pure fr - 008 was obtained. hplc assay a comparison between antibiotic fr - 008 and candicidin has been made by studying the hplc separation profiles of antibiotic fr - 008, candicidin and a mixture of both

    對鏈黴菌fr - 008和灰色鏈黴菌imru3570的rflp分析發現,它們的染色體具有很高同源性; pfge的比較研究發現,這兩個菌株的明顯區別是鏈黴菌fr - 008攜帶有兩個線性質粒,而在灰色鏈黴菌imru3570中則沒有這兩個線性質粒。
  11. Using i - dopa as a specific substrate, phenoloxidase ( po ) from penaeus chinensis hemolymph was purified by gel - filtration and ion - exchange chromatography, and characterized in terms of its molecular weight and enzymatic properties in this study

    本文以中國對蝦( penaeuschinensis )的血淋巴為材料,利用凝膠過濾和離子交換等方法,對酚氧化酶( phenoloxidase )進行了分離純化和生物化學性質研究。
  12. Purification and characterization of phytase from a. niger an 01001 a. niger an01001 was inoculated on solid media and cultivated at 30 for 5 days. proteins were extracted from solid - state fermentation with 50mm acetate buffer ( ph5. 5 ). the molecule weight of the phytase protein was determined as about 78kd by sds - page. the purification procedures include ammonium sulfate precipitation, deae - cellulose ion - exchange chromatography, gel electrophoresis and electroelution

    3 .植酸酶的分離純化及其性質研究黑麴黴ano1001經固體發酵,用緩沖液抽提后,經硫酸按沉澱, deae一纖維素離子交換層析,聚丙烯酞胺凝膠電泳和電洗脫等純化步驟獲得的植酸酶,用sds一page檢測為一條均一譜帶,其分子量約為78kd 。
  13. Glycoproteins were detected from the elution of classified proteins by gel chromatography. glycoproteins from the leaves of camellia sinensis were respectively detected by preparatory quality of gel chromatography and glycoprotein staining in sds - gel

    各分級蛋白進行sephadexg - 100凝膠過濾,使分級蛋白中的蛋白質按其分子量的大小先後洗脫,從每個分級蛋白凝膠過濾的收集液中,鑒定了茶樹葉糖蛋白。
  14. In order to attain bioactive glycoprotein, glycoprotein of the leaves of camellia sinensis, was purified from coarse glycoproteins purified by sephadex g - 100 gel chromatography, by cona - sepharose 4b affinity chromatography

    為了純化天然糖蛋白,進行了茶樹葉糖蛋白的cona - sepharose4b親和層析,從sephadexg - 100凝膠過濾收集的粗糖蛋白中,分離茶樹葉糖蛋白。
  15. Conclusion the co - utilization of silica gel column and gel chromatography can greatly improve the efficiency of isolation and purification

    結論硅膠柱色譜和凝膠柱色譜的混合利用可以提高供試品的分離純化效果。
  16. Methods the optimal elution requirement was found by comparing several different elution agents, and the pure compound was purified by silica gel column and gel chromatography

    方法通過最佳展開劑的選擇,找到硅膠柱色譜系統的最佳洗脫條件,粗分離后的供試品通過凝膠柱色譜系統分離純化得到純供試品。
  17. The examination of the urine specimens by gel chromatography and electrophoresis showed that the active ingredient excreted in the urine was partly unchanged and partly depolymerized to molecules of shorter chain lengths

    用凝膠電泳色譜法檢測尿液顯示,尿液中的活性藥物成分部分為原形,部分解聚成更短的分子鏈。
  18. 1. isolation, purification and characterization of lipovitellin from the ovary of female eriocheir sinensis broodstock lipovitellin was extracted and purified using gel chromatography and electrophoresis procedures from ovaries of chinese mitten - handed crab. one kind of lipovitellin was obtained

    一、中華絨鰲蟹卵黃磷蛋白的分離、純化與鑒定目前在判定蝦蟹卵巢成熟和卵質時,大多採用性腺指數、蛋白質和脂類的組成與含量等生化指標,尚不夠全面。
  19. Metachromasia, electrophoresis, and gel chromatography have shown that the charge of mps and its chain length remain unchanged

    利用藥物的變色反應性,電泳和凝膠色譜法顯示mps (多磺酸粘多糖)的電荷及其鏈長沒有改變。
  20. Second, we used high performance liquid chromatography ( hplc ), thin - layer chromatography ( tlc ), gel chromatography ( gc ), and protein and nucleic acid analytic apparatus to evaluate f i " s purification

    其次,利用高效液相色譜,蛋白核酸分析儀,薄層層析,凝膠色譜等檢測手段,從各個角度入手,證明該f為單一組分。
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