gel filtration chromatography 中文意思是什麼

gel filtration chromatography 解釋
凝膠過濾層析,凝膠過濾色譜法
  • gel : n. 凍膠，凝膠（體）；定型發膠。vi. （-ll-） 成凍膠，膠化。
  • filtration : n. 過濾；滲入。 automatic （centrifugal） filtration 自動（離心）過濾。
  • chromatography : n. 【化學】層析，色層（分離）法。
  1. It was very slow at 5 but become higher above 25. the enzyme from abomasums used in cheese production was rough extracts containing chymosin and pepsin. through the gel filtration chromatography and ion exchange chromatogra

    羔羊胃蛋白酶最適凝乳溫度為45 』 c ；在45處理30inin ，酶活性開始下降， 60c處理30lliln ，酶活性完全喪失；酶的最適ph為1
  2. The separation of the formica rufa linnaeus samples by pel filtration chromatography. in this part, the author has primarily separated the formica rufa linnaeus samples by glucosan gel g - 50 filtration chromatography. the principle is the difference of the molecular weight of different components

    紅褐林蟻樣品的凝膠過濾層析分離本部分旨在通過葡聚糖凝膠g - 50過濾層析初步分離紅褐林蟻樣品，根據分子量大小不同而達到分離目的。
  3. Strain bl21, and gene expression was induced by iptg. the target proteins were directed into the periplasmic space by the staphylococcal protein a signal sequence preceding the rgd - hirudin gene. using ion exchange chromatography and gel filtration chromatography, the chimera proteins were purified, and both of them showed a single band in tricine - sds - page. the results of activity analysis suggested that these two chimera proteins not only have antithrombin activities, but gain platelet aggregation inhibitory activities as well

    通過離子交換層析和凝膠過濾層分別對兩種嵌合體蛋白進行純化，純化產物在tricine - sds - page中都顯示為單一條帶。活性分析結果表明兩種嵌合體蛋白在保留水蛭素抗凝血酶活力的同時，還呈現抗血小板聚集活性。
  4. 6 - phosphogluconate dehydrogenase ( 6 - pgadase, ec 1. 1. 1. 44 ) was isolated by homogenate, ammunium sulfate fractionation, deae - sepharose chromatography, blue - sepharose affinity chromatography and gel filtration with sephadex g - 200 from bacillus subtilis, and some properties of the enzyme had been studied. a 113. 8 - fold purification was obtained with a 8. 2 % yield. the purified enzyme moved as a single electrophoretic band in page

    將枯草芽孢桿菌超聲波破壁后的粗提取物進行分段鹽析、 deae - sepharose陰離子交換柱層析， blue - sepharosecl - 6b特異結合柱層析和sephadexg - 200凝膠過濾等純化步驟，得到聚丙烯酰胺凝膠電泳為單一蛋白區帶，比活為1 . 46u mg的酶制劑。
  5. The xerocomus spadiceus lectin, xsl, was isolated from extracts of fruiting bodies of the mushroom xerocomus spadiceus using a procedure that involved ( nh4 ) 2so4 precipitation, anion exchange chromatography on deae - cellulose, affinity chromatography on affi - gel blue gel, cation exchange chromatography on cm - cellulose, and gel filtration by fast protein liquid chromatography on superdex 75

    從磚紅絨蓋牛肝菌（ xerocomusspadiceus ）子實體粗提物中，經過deae -纖維素陰離子交換層析、 affi - gelbluegel親和層析、 cm -纖維素陽離子交換層析和superdex75fplc凝膠過濾，純化了磚紅絨蓋牛肝菌凝集素。
  6. Using i - dopa as a specific substrate, phenoloxidase ( po ) from penaeus chinensis hemolymph was purified by gel - filtration and ion - exchange chromatography, and characterized in terms of its molecular weight and enzymatic properties in this study

    本文以中國對蝦（ penaeuschinensis ）的血淋巴為材料，利用凝膠過濾和離子交換等方法，對酚氧化酶（ phenoloxidase ）進行了分離純化和生物化學性質研究。
  7. Crotalaria mucronata lectin ( cml ) was purified from seeds of crotalaria mucronata by extraction, fraction with ( nhi ) 2864, hog gastric mucin - sepharose 4b affinity chromatography and followed by gel filtration of sephacryl s - 200 hr. cml agglutinated type a human red blood cells specially. the purified cml gave one band pel e ' ectronhoresis and on sds nolvacrvlamide gel electrophoresis

    野花生豆（ crotalariamucronata ）經磨粉、浸取、硫酸銨分級沉澱、豬胃粘蛋白- sepharose4b親和層析、 sephacryls - 200hr分子篩層析可得到一表觀分子量為103kd且對a型血紅細胞專一凝集的野花生豆凝集素（ crotalariamucronatalectin ， cml ） 。
  8. Under these conditions the fibrinolytic activity of the supernatant can reach 820 urokinase units per milliliter broth. ba - dfe was purified from the supernatant of b. amyloiquefaciens dc - 4 culture broth by ammonium sulfate precipitation, ion - exchange chromatography on cm - and deae - sepharose fast flow, hydrophobic interaction chromatography on phenyl sepharose 6 fast flow and gel filtration on sephadex g - 50. the purified enzyme displayed thermophilic, hydrophilic and strong fibrinolytic activity

    通過硫酸銨分級沉澱、 cm - sepharosefastflow和deae - sepharosefastflow離子交換層析、 phenylsepharose6fastflow疏水層析和sephadexg - 50凝膠過濾等方法，從解澱粉芽孢桿菌dc - 4的發酵液中分離純化出電泳純的ba - dfe 。
  9. The enzyme activity in fermentation liquid could be inhibited by pmsf and dfp. the fermentation liquor also showed good dehairing activity. the alkaline protease ( named dhap, dehairing alkaline protease ) in the fermentation liquid was purified with hydrophobic interaction chromatography, ion exchange and gel filtration

    通過cm - sepharosefastflow離子交換層析， deae - sepharosefastflow離子交換層析， sephacryls - 100 ， sephacryls - 200凝膠過濾層析，疏水層析等純化步驟對短小芽孢桿菌發酵液中的堿性蛋白酶進行了純化。
  10. Gel filtration chromatography, gfc

    凝膠過濾色譜法
  11. Gel filtration chromatography

    凝膠過濾色譜法
  12. We have sifted 103 medicinal plants, roughly identified 17 plants might contain antifungal proteins. antifungal protein was purified from cassia sophera linn, by extraction, fraction with ( nh _ ( 4 ) ) _ ( 2 ) so _ ( 4 ), cation - exchange chromatography of cm - sepharose ff xk 26, the first cation - exchange chromatography of mono s and the second one, followed by gel filtration of superose 12hr

    對茳芒決明進行了抗菌蛋白的分離純化：經粉碎、磷酸緩沖液浸提、硫酸銨沉澱、 cm - sepharoseffxk26陽離子交換層析、兩次monos陽離子交換層析、 superose12hr分子篩層析可得到具抗真菌活性的蛋白。
  13. The antimicrobial secretion could be divided into three sections, every section had antimicrobial activity. the first could be extracted by petroleum ether, the second could be extracted by ethyl alcohol, the third could dissove in water and could be separated by sephadex g - 50, g200 gel filtration chromatography and carbornmethyl cellulose - 1 anion - exchange chromatography and detected by a256, . the antimicrobial secretion had wide spectrum and had strong inhitory activity against germs and fungi, they could inhibit sixteen kinds of plant pathogenic germs, eight kinds of animal germs and eight kinds of plant pathogenic fungi

    粗提物經石油醚萃取可得第一個活性部分，剩餘部分經無水乙醇萃取可得第二個活性部分，剩餘物質再經凝膠sephadexg - 50後有兩個峰，第二峰有活性，再經凝膠sephadexg - 200後有三峰，第二峰有活性，將其經過陽離子交換柱cm - 1後有兩峰，此兩峰均有抑菌活性。
  14. One is a combination of ion exchange chromatography on q sepharose ff, hydrophobic interaction chromatography on phenyl hp, gel filtration chromatography on sephadex 200 and higher resolution ion exchange chromatography on mono q. the other is a combination of ion exchange chromatography on q sepharose ff, two affinity chromatography on con a sepharose 4b and benzamidine sepharose 6bcl sequentially

    上清液中重組蝗蛇毒類激血酶的純化是通過設計的不同分離純化方法組合進行的，並對每一種組合進行了活力與純度及口收率的評價，結果表明兩種組合方式都具有一定的實用性和可操作性。
  15. Bioassay showed that the second - stage juvenile ( j2 ) could be killed by the raw extract of serine protease. serine protease was characterized after purification with ion exchange chromatography and gel filtration chromatography. the t profile, t stability profile, ph profile, substrate specificity and inhibitor were tested

    該酶分子n -端的氨基酸序列為avidtgveashpef ，通過n -端氨基酸序列設計簡並引物，提取被毛孢owvt - 1的總rna ， rt - pcr克隆了該酶的成熟肽編碼基因（ 1000bp ） ，序列分析表明，絲氨酸含量高達12以上。
  16. The purified badh, which became a single band in sds - page, has been obtained by a combination of ammonium sulfate fractionation, ion exchange chromatography and gel filtration chromatography

    遼寧堿蓬甜菜堿醛脫氫酶分子量為129kd ，單個亞基的分子量為64 . 5kd ，表明其為同型二聚體。
  17. The brine shrimp he was prepared and purified by 67 % ammonium sulfate precipitation, deae - sepharose fast flow ion - exchange chromatography, and sephacryl column gel - filtration, and its biochemical and enzymological properties were identified in this study. it was found that the deduced molecular weight of he in sds - page is about 98. 5 kda, and its proteolytic activity was optimized at ph of 7. 5 - 8. 5 and at temperature of 40, respectively

    我們利用67硫酸銨沉澱、 deae - sepharosefastflow陰離子交換柱層析和sephacryl凝膠過濾柱層析，並以酪蛋白為其蛋白酶水解活性的檢測底物、以卵膜為其卵殼裂解活性的特異性底物，從鹵蟲胚胎孵化液中分離純化出了鹵蟲的孵化酶分子，其在sds - page電泳中的分子量約為98 . 5kda 。
  18. 3. the extracellular phb depolymerase was purified from 09 by using hydrophobic column chromatography and gel filtration technique in sephadex g - 100. the specific activity of the purified enzyme was increased by 37. 9 folds over crude extract, and the recovery yield was 8. 9 %

    以粗酶液為起始，經硫酸銨分級沉澱、 sephadexg - 100凝膠過濾后，分離純化了該酶，純化倍數約為37 . 9 ，酶活力回收率8 . 9 。
  19. The results of superose 6 gel - filtration chromatography and western blot suggest that the deletion mutant cause a gigantic change in global structure of arog

    第三部分工作研究了戶四g結構上的「 dz 」對稱性在反饋抑制中的作用。
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