gel fraction 中文意思是什麼
gel fraction
解釋
膠凝部分-
This material was loaded onto a silica gel column and the column was eluted with chloroform several times until the fraction of interest was collected. finally we obtained some light yellow green ointment liquid f i
採用硅膠作干層析柱填充物，用氯仿作展開劑和洗脫液，反復硅膠層析，對柄海鞘鞘囊氯仿浸提物進行分離、提取與純化，最後得到一淺黃綠色的油狀液體fraction （以下均稱f ） 。 -
Crotalaria mucronata lectin ( cml ) was purified from seeds of crotalaria mucronata by extraction, fraction with ( nhi ) 2864, hog gastric mucin - sepharose 4b affinity chromatography and followed by gel filtration of sephacryl s - 200 hr. cml agglutinated type a human red blood cells specially. the purified cml gave one band pel e ' ectronhoresis and on sds nolvacrvlamide gel electrophoresis
野花生豆（ crotalariamucronata ）經磨粉、浸取、硫酸銨分級沉澱、豬胃粘蛋白- sepharose4b親和層析、 sephacryls - 200hr分子篩層析可得到一表觀分子量為103kd且對a型血紅細胞專一凝集的野花生豆凝集素（ crotalariamucronatalectin ， cml ） 。 -
We have sifted 103 medicinal plants, roughly identified 17 plants might contain antifungal proteins. antifungal protein was purified from cassia sophera linn, by extraction, fraction with ( nh _ ( 4 ) ) _ ( 2 ) so _ ( 4 ), cation - exchange chromatography of cm - sepharose ff xk 26, the first cation - exchange chromatography of mono s and the second one, followed by gel filtration of superose 12hr
對茳芒決明進行了抗菌蛋白的分離純化：經粉碎、磷酸緩沖液浸提、硫酸銨沉澱、 cm - sepharoseffxk26陽離子交換層析、兩次monos陽離子交換層析、 superose12hr分子篩層析可得到具抗真菌活性的蛋白。 -
Nanosized lead titanate ( pt ) and lead titanate doped with calcium and lanthanum ( pclt ) powder obtained by the sol - gel method was added into vinylidene fluoride - trifluoroemylene [ p ( vdf - trfe ) ] to form nanocomposite to be used as the sensing film of the pyroelectric detector. the experiment results show that after mixing in 0. 16 volume fraction of pclt powder, the pyroelectric coefficient of pttp ( vdf - trfe ) composite film was about 37 % higher than that of the pure p ( vdf - trfe ) film prepared under the same condition, and the voltage figure - of - merit of pt / p ( vdf - trfe ) composite film was about 22. 4 % higher
用溶膠-凝膠法制備了鈦酸鉛（ pt ）和摻鈣鈦酸鑭鉛（ pclt ）納米粉粒，與聚偏氟乙烯-三氟乙烯（ p （ vdf - trfe ）均勻復合，由於16的摻鈣鈦酸鑭鉛納米陶瓷粉粒的摻入，使得pclt / p （ vdf - trfe ）復合膜的熱釋電系數值提高了約37 ，探測優值提高了約22 . 4 。 -
Method was investigated to extract multiple proteins from complex system including water - soluble fraction of egg yolk and agkistrodon acutus venom by bacterial specific absorption. immunoglobulin yolk ( igy ) were absorbed directly from water - soluble fraction by streptococcus mutans, then eluted and further purified by columns of thiophilic gel. based on above experiments of antibody extraction, bacterium cells were applied further to extract interactive proteins from crude venom of agkistrodon acutus
首先，利用變異鏈球菌（ s . mutans ）與其特異性抗體的吸附作用從水溶性蛋白組分中分離出卵黃抗體（ igy ） ，並將菌體上洗脫下來的igy用嗜硫色譜分離純化，得到高純度的細菌特異性抗體。 -
Major difference of hn proteins lies in no. 18 - no. 75 amino acid residues on n - terminal which includes three active sites of hn protein. the influence on biological action, caused by the difference of hn protein, is needed to study further. hn gene has only four glycosylation sites, which is 1 or 2 less on amount than that of other strains. so, this difference can be take on as a natural mark of ndv b95 strain furthermore, the fragment encoding hn gene was excised from the positive clone pgem - hn with sail and saci enzyme, and purified by agrose gel fraction method. then the fragment was subcloned into the pet - 28c expressing vector digested by sail and saci restriction enzyme
作者將正確的重組克隆質粒pgem - hn用saii 、 saci雙酶切，電泳回收目的片段，將其亞克隆到經saii 、 saci雙酶切處理的pet - 28c中，轉化大腸桿菌tgi感受態細胞，得到的轉化子經pcr鑒定和酶切分析，篩選出符合閱讀框的重組子，構建成重組表達質粒pet - hn ，並在大腸桿菌bl _ （ 21 ） （ de _ 3 ）宿主菌中成功地表達了含目的蛋白的融合蛋白，融合蛋白的分子量約66kd ，加入iptg誘導8h后，蛋白表達幾乎達到最高水平。 -
A surface antigen hemagglutinin - neuraminidase ( hn ) gene of the ndv b95 strain, was selected to study as target gene. referred to the reported sequence, rwo primers were designed and synthesized. hn gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fraction method
為此，從澳大利亞某大學獸醫病理系引進一株ndv熱穩定性天然弱毒b95株，並選取其表面抗原血凝素?神經氨酸酶的編碼基因為目的基因，根據國外已發表的序列，設計一對引物，利用rt - pcr技術擴增出了ndvb95株hn基因。
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