gel layer 中文意思是什麼
gel layer
解釋
凝膠層-
In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。 -
Secondly, it will be better to use breathable and high effective gel layer for the middle layer
第二，中層以透氣、內含高效膠化層的為好。 -
The pamps gel layer swells - shrinks reversibly as the solution environments ( ph and i ) change. the water flux increases with decreasing ph and decreases with 7
在凝膠復合膜過濾bsa溶液的實驗中，離子強度減小，截留率升高；離子強度增大，截留率下降。 -
Aiming at characteristics of serious conflict among layers, thin layers developed and scattered distribution of remaining oil, this paper introduces techniques of non - gel fracturing, multi - fractures fracturing, large discharge capacity and large sand amount fracturing, thin layer fracturing and high effective return discharge
摘要針對大慶油田薩南開發區三類油層層間矛盾突出、油層發育薄及剩餘油分佈零散等特點，根據油藏精細地質研究成果，通過個性化壓裂設計，在試驗區三類油層聚驅挖潛過程中，應用了非凝膠壓裂、多裂縫壓裂、大排量及大砂量壓裂、薄隔層壓裂以及高效返排壓裂等配套的工藝技術。 -
Design and analysis of optical anti - reflective multi - layer coatings derived from sol - gel process
多層高反膜的理論設計與光學特性分析 -
The sanitary napkin with high effective gel layer can congeal the infiltrating liquid to jelly, not flow back after pressing, and you won ' t feel sticky on the surface
內含高效膠化層的衛生巾，可把滲入的液體凝結成?喱狀，受壓后不回滲，表面沒有粘乎乎的感覺。 -
In the experiments, two series of catalysts were synthesized by co - deposition and sol - gel methods each. the composition of both waterproof and gas diffusion layer and catalysis layer were optimized by the orthogonal test. meanwhile, quasi - steady cathode polarization curves, x - ray diffraction ( xrd ), scanning electronic microscope ( sem ) were used to analyst the relationship between the microstructure and the performance of air electrode
實驗採用共沉澱法和溶膠凝膠法合成了兩類催化劑;對防水透氣層和催化層進行了正交試驗優化;同時，還採用測準穩態極化曲線， x射線衍射（ xrd ） 、掃描電子顯微鏡（ sem ）等分析測試手段，對空氣擴散電極催化材料、空氣擴散電極界面結構與性能的關系等作了較深入的研究。 -
The crosslinking system was : [ mbaa ] = 0. 03, 0. 06, 0. 09mol / l, [ k2s2o8 ] = 0. 0075 mol / l, t = 60 c, t = 40 ~ 60min. the existence of pamps gel layer on the surface of composite membrane was confirmed by ftir and sem
通過傅立葉紅外光譜（ ftir ）分析和掃描電子顯微鏡（ sem ）的觀察，證實了pvdf基膜表面pamps凝膠層的存在。 -
Differentiation of black gel pen inks by thin layer chromatography
薄層色譜法鑒別黑色中性筆色痕的種類 -
Gel thin layer chromatography
凝膠薄層色譜法 -
Second, we used high performance liquid chromatography ( hplc ), thin - layer chromatography ( tlc ), gel chromatography ( gc ), and protein and nucleic acid analytic apparatus to evaluate f i " s purification
其次，利用高效液相色譜，蛋白核酸分析儀，薄層層析，凝膠色譜等檢測手段，從各個角度入手，證明該f為單一組分。 -
In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals
首先，用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上，並轉化e colidh5a感受態細胞中，經pcr 、酶切以及測序證明得到了完整的2c3abc基因，並與國內外參考序列進行比較分析。然後，將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ，以zeocin ~ （ tm ）抗性篩選陽性克隆，大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞，通過zeocin ~ （ tm ）抗生素梯度濃度篩選，獲得重組酵母用0 . 5甲醇誘導表達，通過sds - page電泳、 westernblotting分析，結果表明， 2c3abc基因在畢赤酵母中成功表達，其表達產物為一95ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。 -
In this articles, we research the all - solid state electrochromic device which ec layer is wo3 doped with tio2 moo3 cro3 peo, ce layer is ceo2 doped with tio2, and ic layer is epichlorohydrin crosslinked polyethenoxy ether gel macro - molecule electrolyte. in this articles, we research the color change, responding time, electrochemistry performance, preserving time, and lay importance on the research in the circle times, crystal structure and the uniformity, stability and suitable viscosity of precursors
本文首次研究了wo _ 3以為主體， tio _ 2 、 moo _ 3 、 cro _ 3 、 peo摻雜的電致變色層的性能，以tio _ 2為主體的對電極變色層的性能和以liclo _ 4 -環氧氯丙烷交聯聚氧乙烯醚為高分子固體電解質的全固態電致變色器件。 -
Ectoplasm the outer gel - like layer of the cytoplasm in the cells of plants and some protoctists, which lies immediately beneath the cell membrane and contains a dense array of microtubules
外質：植物及某些原植體細胞內，細胞基質近外方的膠狀層，直接位於細胞膜之下，並含有密集排列的微管。
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