gene addition 中文意思是什麼

gene addition 解釋
基因添加
  • gene : n. 【生物學】基因。 dominant gene顯性基因。
  • addition : n. 1. 附加，追加；附加物。2. 【數學】加法，加算。3. 【法律】（加在姓名后的）頭銜，官銜；稱號。4. （建築物的）附加部分。
  1. In addition to avermectins, s. avermitilis produces oligomycin, a strongly toxic compound. gene deletion vector pxl05 was used to disrupt oligomycin polyketide synthase ( pks ) encoding genes ( olma ) in streptomyces avermitilis cz8 - 73, the producer of anthelmintic avermectins b and the cell growth inhibitor oligomycin. olma gene cluster in the chromosome was displaced by deletion allele on the plasmid via double crossover

    本研究以產阿維菌素b和寡黴素的阿維鏈黴菌cz8 - 73為出發菌株，構建了基因缺失載體pxl05 ，並將其轉入cz8 - 73中，通過缺失載體和染色體之間的同源雙交換，對染色體上長達90kb的寡黴素聚酮合酶（ pks ）基因簇（ olma ）進行了缺失。
  2. Since the important roles of eo protein in the viral infection. immunity and virus - host interaction. the homology of 21 csfv strains was investigated by sequence analysis of eo genes in this study, which will provide some evidence for epidemiological study. in addition, the eo gene of hog cholera lapinized vaccine ( hclv ) strain was expressed in the prokaryotic and eucaryotic systems, and the recombinant proteins were preliminarily analyzed by immunological method

    鑒于eo蛋白在病毒感染，誘導機體免疫及與宿主細胞相互關系中的作用，本研究克隆了2株豬瘟病毒eo基因並將其與其它毒株eo基因進行了序列分析，揭示了我國豬瘟病毒流行株之間的遺傳演化關系，為豬瘟病毒的流行病學研究提供依據。
  3. In order to facilitate the study of biological function of add gene cluster, e. coli - s. avermitilis intragenus conjugation system was established. in addition, phz2114 for the replacement of the entire add gene cluster and phz2130 for disruption of adda were constructed

    建立了除蟲鏈黴菌的接合轉移系統，並構建了用於置換全部基因簇的基因置換質粒phz2114和adda的基因中斷質粒phz2130 ，為研究除蟲鏈黴菌add基因簇的生物學功能奠定了基礎。
  4. So must use information theory method depict and abundant the genetic diversity index system. in addition to, the introduce of molecule biology technology and the research of nucleotide sequence evolutive give a new method for population genetic, so must do deeply research about the analysis method of dna sequence data = the research main about the follows : there are three parts about the information model of population genetic : one about the shannon information entropy property of equilibrium population and the entropy change in the process of establish equilibrium ; another research is about the diversity measure - ment of genetic variation ; lastly, research the shannon information measurement about the disequilibrium gene variation. the result is : 1 to a definite gene distribution, the genotype entropy reach the maximum at the equilibrium population, the process of population from disequilibrium to equilibrium, the entropy get large and large

    此外，分子生物技術的介入及核苷酸序列進化的研究都為群體遺傳學的深入研究提供了新的途徑，但關于dna序列數據的分析方法需要作進一步的研究。本研究主要體現在以下幾個方面： （一）關于群體遺傳學的信息論模型研究，主要分為三部分內容：一是群體平衡的shannon信息熵的性質和群體平衡建立的熵變性質；二是群體遺傳多樣性測度的研究；三是非平衡群體的基因變異測量shannon信息量的方法研究。得到了如下結論： 1 、平衡群體的shannon信息熵最大，群體平衡的過程是熵的增大過程。
  5. In addition, in silico studies demonstrated that among many tissues, human brain and testis exhibit a most similar gene expression pattern, suggesting testis might contribute to human speciation and evolution together with brain

    這些結果提示睪丸可能與腦類似，也在人的物種形成和進化歷程中起著重要作用。
  6. In addition, gene expression patterns across brain regions show ariable degrees of restriction to single brain structures

    另外，整個腦區域的基因表現型顯示了單一腦結構的區域可變性。
  7. With the acceleration of extinction of species, biodiversity conservation is extensively concerned. the extinction of species is concerned with the degree of threat by biotic and abiotic factors. so, taking action to preserve plant species is very necessary and paramount before their extinction. one of the most effective biological techniques to conserve the biodiversity is the establishment of genebanks, i. e. ex situ conservation. the elucidation of various factors that regulate seed viability and vigor in storage is essential. an ideal condition to prolong the longevity is mainly depended on seed water content, temperature, humidity and types of containers used during storage. the optimum stage of seed maturity, harvesting techniques and processing, in addition to physiological features such as degree of dormancy, also play key roles in seed storage. certainly, desiccated seeds deteriorate with time even under extremely good genebanking conditions. according to seed storage behavior, it is necessary to consider three principal factors : storage environment, storage duration and plant species which will affect seed survival under good genebanking conditions. the present review is an attempt to discuss the importance of the aforementioned aspects of seeds in detail in order to conserve plant germplasms ( especially wild rare and endangered plants ) for ex situ conservation through seed - gene bank

    一個物種的滅絕是與其受生物因子和非生物因子的威脅程度相關的.隨著物種的加速絕滅，保護生物多樣性受到廣泛地關注.保護生物多樣性的最有效的生物技術之一是建立種子基因庫，進行遷地保護.種子庫理想的貯藏條件主要取決于種子含水量、貯藏環境（如溫度和濕度）和貯存種子的容器.進行種子貯藏，了解種子生命力和活力的影響因子的作用機理是十分重要和必要的.除了種子自身的生理特徵外，種子的貯藏壽命與種子成熟度、收獲技術、加工處理方法也是息息相關的.即使在最適的庫存條件下，種子也會隨時間發生劣變.因此，必須根據種子特定的貯藏行為，加以考慮影響種子存活的3個主要方面（貯藏環境、貯藏期和植物種類）而選擇有效的貯藏方案.本文試圖討論種子貯藏生理的幾個重要方面及其需解決的技術問題，以便更好地通過種子基因庫，長期有效地保存植物種質資源
  8. In addition, the well retained stability and integrity of cell membrane of boea leaves might also be an important mechanism which make them resurrect well. by using mrna differential display, 5 desiccation sensitive cdnas, 52 desiccation - induced cdnas, 21 up - regulated cdnas, 14 down - regulated cdnas and 16 phosphate induced cdnas were obtained. the cloning, sequencing, homological blasting and northern blotting results of 5 desiccation - induced cdnas and 3 phosphate induced cdnas implied that signal transduction induced by desiccation, regulatory gene cascades and functional genes such as g protein, protein kinase, vp3 - and mad3 - like genes might be involved in dehydration in the resurrection plant boea hygrometrica

    對其中5個脫水特異誘導表達牛耳草光合作廠j的脫水保護和復甦機理的cdna （包括可能與復甦能力有關的cdna ）和3個磷酸鹽處理誘導表達的cdna進行克險測序、同源性探測和northern雜交檢測表明，牛耳草脫水過程中誘導表達的基因可能涉及到脫水脅迫的信號轉導「蛋白、蛋白激酶等） 、調節基因的級聯作用（ vp3 ， mad3樣基因等） 、結構基因產物調節細胞結構（包括細胞質膜）在脫水脅迫中的穩定性等。
  9. In addition, base on the certain level of genetic differentiation of among the 4 possible natural populations, it is advised that adult plants or seedlings of ginkgo be transplanted mutually in order to enhance the gene flow among them and preserve the genetic diversity of ginkgo furthest

    因銀杏群體間已出現了一定程度的遺傳分化，建議在可能的自然群體間進行植株和種子幼苗相互移栽，以增強群體間的基因流，最大限度地保護銀杏的遺傳多樣性。
  10. Compared with a reported cmv - cp gene sequence, the homology of nucleotide sequences were 100 %. the sequencing result also demonstrated the recombinant vector pet - 22b - cp has a proper orf encoding 218 amino acids. the recombinant vector was transformed into bl21 ( de3 ) cells. transformants were grown and induced by the addition of isothiopropylgalactoside ( iptg ) to a concentration of imm with continued shaking at 37 ?

    酶切鑒定及序列測定表明，重組表達質粒pet - 22b - cp連接區域符合設計要求，具有正確的開放閱讀框架，插入片段含有218個氨基酸的完整編碼區，其核苷酸序列與報道的cmv - cp基因的同源性為100 。
  11. In this study, a new gene c / wew, encoding cholesterol oxidase, was isolated from rhodococcus equi. 4 - 2g2 found in china, which may be useful in clinical diagnosis healthy food and pest management in agriculture. in addition, the gene has been expressed successfully, the expression product has cholesterol oxidase activity, thus this work provided theoretical basis to the development of genetic engineering of cholesterol oxidase

    本研究從我國自行分離的馬紅球菌4 ? 2g2菌株中分離到一種編碼膽固醇氧化酶的新基因choew ，它將可應用於臨床檢測、保健食品和農業抗蟲等領域；同時利用原核表達載體成功的在大腸桿菌中表達了目的基因，表現出膽固醇氧化酶酶活，為膽固醇氧化酶的基因工程利用開發作了一定的理論實驗基礎研究。
  12. Then, 5. 5kb thrombiotin gene was amplified with the same technique from the genome of a baby ' s blood, which included the begining part of intronl to the teminator. in addition, 6. 0kb and 1. 8kb homlogous arms were also amplified from a cow with high yield. the 6. 0kb homologous arm contains the promotor, extron 1, extron2, extron3 and intron 1, intron2 and part of the intron3 fragment, while the 1. 8kb homologous right arms contains exon13, exon14 and part of intron 13, the whole intron14 and part intron 14 of asl - casein gene of bovine

    通過長片段pcr從高產奶牛的基因組中獲得了打靶所需的長、短同源臂序列，長度分別為6 . 0kb和1 . 8kb ，位於s1 -酪蛋白基因的5上游區到第三內含子和十二到十四內含子；從綿羊全血基因組克隆得到了綿羊的-酪蛋白基因啟動子區到第二內含子區4 . 1kb的5調控序列；利用同對引物克隆得到了水牛的同基因序列；從廣西當地一嬰兒臍血基因組中通過獲得了人血小板生成素基因，位於第1內含子到終止子後部分的序列，長達5 . 5kb 。
  13. In addition, we also focused on constructing a plant virus - based gene vector pvx to express ltb - st fused gene by means of agroinfection. the possibility of producing vaccine was probed through plant virus - based gene vector expression system

    同時本研究還通過構建ltb ? st融合基因pvx病毒表達載體，並用農感染法轉化植物，探索了暫態表達系統生產疫苗的可能性。
  14. These include cell growth characteristics, expression levels, intracellular and extracellular expression, posttranslational modifications, and biological activity of the protein of interest, as well as regulatory issues in the production of therapeutic proteins. in addition, the selection of a particular expression system requires a cost breakdown in terms of process, design, and other economic considerations. section i : construction of pet22b ( + ) / hpk - 5 vector the hpk - 5 gene encoding 82 amino acid residues from c462 to c543 was recombined with the sequence of plasmid pet22b ( + ) for constructed a new expressed vector pet / hpk - 5

    方法在對hpk - 5 （ humanplasminogenkringle5 ， hpk - 5 ）因子的基因序列和蛋白質序列進行分析的基礎上，利用pcr技術分別構建其可溶性原核表達載體和不溶性原核表達載體；用pcr快速檢測法及其基因測序儀測序以鑒定pet22b hpk - 5和pbv220 hpk - 5重組質粒，用不同的感受態大腸桿菌（ e
  15. Because there are many copies of chloroplast dna and the chloroplast has a strong tolerance to accumulation of the products expressed by the introduced foreign gene, a high level of expression is often happened in chloroplast transformation. in addition, because of prokaryotic property of the chloroplast, the prokaryotic gene can be expressed in chloroplast without any modification and multigene can be simultaneously transferred in " polycistron ", which is impossible in nucleic transformation

    另外，由於葉綠體基因組的原核性質，對來自原核生物的外源基因無需改造就可以在葉綠體內高效表達，而且可以將多個外源基因採取「多順反子」的原核表達形式同時引入，並由共同的啟動子控制，既方便操作又可避免由於存在多個相同啟動子所帶來的「共沉默」 。
  16. Intergenomic gene transferring for early - morning flowering from o. latifolia to o. sativa by common hybridization method and studying on the early - morning flowering lines of o. sativa with the monosomic alien addition of o. latifolia

    闊葉野生稻黎明開花特性的轉育和栽培稻早晨開花系的研究
  17. In addition, instead of just examining what happens with one specific gene if it is turned on or off, they were able to directly compare the effects of remoing different genes on organ formation and function

    此外，對於一個特定基因是否被打開或者被關閉僅僅是檢查一下已經被取代，他們可以直接比較移除在器官形成和功能時不同的基因時的效果。
  18. In addition, instead of just examining what happens with one specific gene if it is turned on or off, they were able to directly compare the effects of removing different genes on organ formation and function

    此外，對於一個特定基因是否被打開或者被關閉僅僅是檢查一下已經被取代，他們可以直接比較移除在器官形成和功能時不同的基因時的效果。
  19. The genomics dna of the transformants was extracted and assayed by pcr with nptii primer camv35 / cp primer and the results indicated that the chloroplast shsp gene has been integrated into the genomics of the tomato. then the transgenic tomato were exposed to low temperature ( in winter, on natural condition, the top temperature was 15 ? and the lowest temperature was 5 and a set of physiology parameters were measured after 6 weeks. the results were shown as follows : 1 ) effect on growth height of the transgenic tomato and the control plants after 6 weeks at low temperature showed that the transformants had been grown faster than the control. in addition, the leaves of the control plants appeared to be much reder than the transgenic tomato, and the change were obvious followed by far from the treated time at low temperature, which suggested that the constituently expression of the chloroplast shsp had some protective fountions to the tomato at low temperature

    提取轉基因番茄基因組dna ，分別以npt和35s cp引物對其進行pcr分析，結果表明葉綠體shsp基因已整合進番茄基因組中；對轉基因番茄進行低溫處理（冬季，自然條件下（無加熱的溫室） ，白天最高溫度15 ，夜間最低溫度5 ） ，生長6周后，檢測轉基因番茄的系列生理指標，主要結果如下： 1 ）生長勢：測量轉基因番茄與對照（未轉基因番茄）的株高，結果顯示轉基因植株生長明顯快于對照，且從外觀上看到對照葉片發紅程度遠大於轉基因植株，隨著低溫時間延長，對比更加明顯，說明葉綠體shsp的組成性表達在低溫下對番茄具有一定的保護作用。
  20. And strain ss - ori is sinorhizobium sp. according to the " blast " data. in addition, a hydantoinase gene ( 1440bp ) from yz - ii6 was amplified by pcr with genomic dna as the template

    我們還利用pcr方法從菌株yz - 6基因組中克隆得到了海因酶的基因（ 1440bp ） ，並構建了表達質粒pet3a - hdtase 。
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