gene cloning 中文意思是什麼

gene cloning 解釋
基因克隆化
  • gene : n. 【生物學】基因。 dominant gene顯性基因。
  • cloning : 基因轉殖 克隆
  1. Gene cloning provides a means of purifying and propagating specific dna segments.

    基因克隆化提供了一種純化和擴增特定DNA片段的方法。
  2. Molecular biology reagents of gene cloning 4

    基因克隆相關分子生物學試劑4
  3. This method can be widely applied in type ii pha synthase gene cloning

    該方法的特點是簡單易行、重復性好且適用性強。
  4. Human liver glutathione - s - transferase m1 gene cloning and temperature - dependent expression in e. coli

    1基因的克隆及在大腸桿菌的溫控表達
  5. Recent studies show that dna transfer and recombination technology provides great potential for genetic improvement of zoysiagrasses ; molecular linkage maps were constructed from zoysia japonica and its hybrids with zoysia matrella ; the researches on gene cloning and gene resource are in progress

    最近的研究表明,基因轉移與重組技術在結縷草遺傳改良中具有巨大應用潛力;結縷草遺傳連鎖圖譜構建取得重要進展;基因克隆和基因資源研究正在展開。
  6. By the technology of gene cloning, bioconversion of d - amino acids with engineered cells containing d - hydantoinase and d - carbamoylase would be expected to overcome the drawbacks presented by using the original strains described above. according to the reported amino acid sequence of d - hydantoinases, two primers were designed and synthesized

    本文根據文獻報道的海因酶基因序列及大腸桿菌對密碼子的偏愛性分別設計了正向及反向引物,以基因組dna為模板,利用pcr技術擴增得到菌株pseudomonasputidayz - 26和sinorhizobiummorelensess - ori的d -海因酶基因。
  7. Gene cloning and expression of outer membrane protein of helicobacter pylori

    幽門螺桿菌外膜蛋白的基因克隆及表達
  8. At last, ctab - dna and sds - dna methods are used in this experiment. on the basis of optimizing experiment procedure and pcr system of the materials, the initial reversion transcription system and orthologus gene cloning technique are established

    對這3種方法比較后,確定本實驗採用ctab - dna酶消化法和sds - dna酶消化法提取白樺雄花芽組織的總rna 。
  9. Gene cloning and expression of pka catalytic subunit and its activity in arabidopsis thaliana

    克隆和表達及鈣調素結合活性分析
  10. Gene cloning differential fragment gha27 was produced by cdna - aflp method employed to compare the gene expression in developing anthers between the male sterile and fertile plants of cotton dong a. a cdna clone, designated gharf ( gossypium hirsutum adp - ribosy lation factor ), was isolated from a cotton ( gossypium hirsutum l. ) cdna library using gha27 as probe. 2

    棉花arf基因的克隆選取棉花洞a雄性不育株和可育株花藥cdna - aflp差別顯示的差異片段gha27 ,用地高辛標記作為探針,篩選棉花洞a花藥的cdna文庫,克隆得到了棉花arf基因的cdna全長序列,將其命名為gharf ( gossypiumhirsutumadp - ribosylationfactor ) 。
  11. Not only sequence information from the c - terminus of proteins and peptides is of especial interest in the investigation of n - terminally blocked proteins and peptides, but also c - terminal sequence analysis can facilitate the production of more specific probes for gene cloning

    C端與n端一樣,在蛋白質分子結構分析中具有重要的地位,對其順序測定具有重要的意義,不僅可以對n端封閉的蛋白質進行序列測定,而且對基因克隆有指導意義。
  12. The article describes some advances in the research of douchi fibrinolytic enzyme. it focuses on screening of strain, enzyme production and purification, gene cloning and expression, enzyme property and pharmacodynamics

    摘要對豆豉溶栓酶的菌種選育、產酶條件、分離純化、基因的克隆與表達、酶學性質以及藥效試驗的研究進展進行綜述。
  13. In this paper, we summarized the research advances on the gene cloning, sequence and structure analysis, function identification, expression and regulation of the ion transporting proteins and focused on their functions in the process of absorption, distribution and translocation of nitrogen, phosphorus, potassium and other nutrients in arabidopsis thaliana

    文中綜述了擬南芥中養分離子轉運蛋白在基因克隆、序列與結構分析、功能鑒定、表達與調控方面的研究進展,其中著重討論了這些轉運蛋白在氮、磷和鉀等營養元素吸收、運輸、分配中的作用。
  14. The works currently in carrying out are the respiratory effect of ischemia of brainstem and its mechanism and the gene cloning and expression and functional identification of neurotrophin 6

    腦幹缺血性呼吸效應及其機制,面神經核的非運動性調節功能,神經營養素的基因表達和功能鑒定等。
  15. Results there are wide applications for medicinal plant researches, such as identification, genetic relationship, origin and evolution, genetic diversity, gene mapping, gene cloning and genetic engineering of metabolic pathway

    結果分子標記在藥用植物的鑒定、親緣關系、起源進化、遺傳多樣性、基因定位及克隆、代謝途徑的基因工程研究等方面有廣泛的應用空間。
  16. Abstract : this paper reviewed the recent advance of the application of dna molecular marker in various aspects of tomato breeding including genetic map construction, germplasm research and purity control of cultivars, identification markers linked to important genes and map - based gene cloning

    摘要本文綜述了dna分子標記在番茄遺傳圖譜構建、番茄種質資源研究與品種純度的鑒定、番茄基因分子標記研究及番茄基因圖位克隆方面的應用研究進展。
  17. Gene cloning and sequence analysis of chitinase gene in wheat seed

    小麥種子中幾丁質酶基因的擴增及序列分析
  18. Ceu line 2 ) molecular biology reagents of gene cloning 3 ) reagents for northern blot and rt - pcr 4 ) cell transfection kit 5 ) reagents for kinase assay 2

    肝癌bel7402細胞系和cos7細胞系2基因克隆相關分子生物學試劑3 northern雜交及rt - pcr相關試劑4
  19. The gene cloning and sequence analysis of senv - d and senv - h isolated from china according to the published nucleotide sequences of sen viruses, specific primers were designed and synthesized. from the serum of two chinese patients with non - a - e hepatitis, one senv - d isolate named senv - d - bj1 spanning the complete coding region was amplified by semi - nested pcr, another isolate named senv - d - bj2 spanning the partial coding region ( including orf1 and orf2 ) was amplified too. from one blood donor serum, two senv - h isolates named senv - h12 - 1 and senv - h 12 - 2 spanning the complete coding region were amplified by nested pcr respectively

    Sen病毒d和h亞型中國分離株的克隆及序列分析我們在前期工作的基礎上,結合已發表的文章及基因序列,針對senv - d和senv - h基因組設計特異性引物,利用套式pcr技術從兩例non - a - e肝炎患者血清中分段克隆得到了一個senv - d亞型分離株( senv - d - bj1 )的全部編碼區基因序列,還得到了一個senv - d亞型分離株( senv - d - bj2 )的大部分編碼區基因序列(包括orf1和orf2 ) ;從一例健康人血清中分段克隆得到了兩個senv - h亞型分離株( senv - h12 - 1和senv - h12 - 2 )的全部編碼區基因序列。
  20. Dominating methods in wheat gene cloning include homology - based cloning, map - based cloning, suppression subtractive hybridization, ddrt - pcr etc

    目前,小麥基因的克隆方法主要有同源克隆、圖位克隆、差減雜交、差異顯示等。
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