gene copy 中文意思是什麼

gene copy 解釋
基因副本
  • gene : n. 【生物學】基因。 dominant gene顯性基因。
  • copy : n 1 抄本，繕本，謄本，摹本，復製品，【電影】拷貝；【法律】副本 （opp script）。2 （書的）一部，一...
  1. The characteristics of tm - 22 expression presented in transgenic tobacco : 1 ). virus specificity in either homozygote or heterozygote ; 2 ) tm - 22 gene integrated in tobacco genomic dna with single copy and in inheritance and segregation to progenies on the mendel role ; 3 ). transgenic line with tm - 22 promoter ( ptm47 ) showed infected symptoms with cell death distinguished to one with 35s promoter ( ptm49 ) after inoculation with tomv - 2a

    其次，通過氨基酸序列和結構的比較，確定tm - 2 ~ 2基因的編碼蛋白與tomv病毒在抗病反應中相互識別的特異氨基酸及其功能；然後，應用重組dna技術，互換tm - 2 ~ 2基因和tm - 2基因的對應結構域，構建嵌合基因，獲得嵌合蛋白表達的轉化體，驗證tm - 2 ~ 2編碼蛋白中變異氨基酸的作用。
  2. Considering alcaligenes faecalis pencillin g acylase ( afpga ), which possesses the attractive characteristics for beta - lactam antibiotics conversions, the gene of pga was cloned into two expressing vector pkkfpga and psmlfpga. both the constructed plasmids psmlfpga and pkkfpga contained the pga gene, trc promoter and rrnb transcript terminator, differ in the replicon and antibotic marker, pkkfpga contained multicopy replicon ( cole 1 ) and ampicillin marker. while psmlfpga contained medium - copy replicon ( p15a ) and tetracycline marker

    本文將糞產堿桿菌青霉素g酰化酶（ afpga ）基因構建表達載體pkkfpga和psmlfpga ， pkkfpga和psmlfpga均含trc啟動子、青霉素g酰化酶基因、 rrnb轉錄終止子，其中pkkfpga含有氨卞青霉素抗性基因和cole1高拷貝復制子；而psmlfpga含有四環素抗性基因和p15a中拷貝復制子。
  3. 1. because the taxonomic division is rather complex and has been much disputed and revised, in this part, we will review the classification and phylogeny of families, subfamilies and tribes of anseriformes based on morphology, ethology, osteology, mitochondrial and nuclear dna restriction fragment length polymorphism, single - copy nuclear dna hybridization and the sequences of mitochondrial gene analysis referring to the different definition, classification and phylogenetic relationships of the families, subfamilies and tribes of anseriformes. the controversial questions and deficiency in the systematic studies of anseriformes were pointed out

    具體包括以下幾個部分： 1 、針對雁形目鳥類異常復雜的分類狀況及分類上存在的爭議，根據雁形目鳥類的形態學、行為學、骨骼學、角蛋白、線粒體與核dna酶切片段長度多態、單拷貝核dna - dna雜交及線粒體基因dna序列分析等方面的研究，對雁形目鳥類分類中科、亞科和族的劃分及其相互間的系統發生關系進行綜述，分析系統學研究中存在的不足，提出了雁形目鳥類分類中急需解決的問題。
  4. 4. engineering dhqase ( arod ) - deficient e. coli mutant with a second copy of the arob gene gene targeting technique was used to disrupt the arod gene in e. coli chromosome. the mutant 31bk was engineered, in which homologous recombination of the arobkanr gene cassette into the arod locus ( arod : : arobkanr ) of the e. coli strain atcc31884 genome utilized the helper plasmid pkd46 with red system. the host cell 31bk lacked catalytic activity of dhqase ( arod ) and had a second copy of the arob gene, so it improved carbon flow into the quinic acid biosynthesis direction

    構建宿主菌基因精確定位突變株31bk （ arod : : arobkan ~ r ）為了改變代謝途徑脫氫奎尼酸（ dhq ）分支點上的代謝流量，使之充分流向目的產物奎尼酸合成方向，利用基因打靶技術構建了31884宿主菌arod基因精確定位插入突變體，使dhq脫水酶（ dhqase ）失活，阻斷了碳代謝流流向芳香氨基酸生成的方向，同時用同源重組的方法將arob基因定位整合入染色體上，解除了限速酶對碳代謝流通過共同途徑到達dhq的阻遏影響，並減輕代謝負擔。
  5. The mechanisms of gene silencing include copy numbers and configuration of transgene, integration sites in plant and transcription of transgene, etc

    基因沉默的機制是多方面的，包括轉基因的拷貝數和構型、在植物上的整合位點、轉基因的轉錄水平等。
  6. The genomic osbhlhl gene is a unique copy gene in rice genome with four introns

    Osbhlh1基因的在基因組序列中是單拷貝的，有4個內含子。
  7. The whole gene was added every six hours instead of all in one time. in proper time, they were mixed and continued to ligate to multi - copy in the same direction

    通過控制基因和接頭加入的量及次序，可得到兩側有ecor和sal酶切位點的同向串連的多拷貝基因。
  8. Objective : construct high - level expression system of echistatin in e. coli methods : obtain amino - acid sequence of echistatin from genebank database. considering the bias of usage of 61 available aminoacid codons in e. coli, design the coding sequence of echistatin, synthesize the dna sequence chemically, get single copy coding gene and repeated two copy coding gene of echistatin. insert the sequence into expression vector pbv220, and more, we construct fusion expression clone of echistatin with pcr, identify the recombinant vector by dna sequencing

    目的構建蛇毒鋸鱗蝰素（ echistatin ）的原核高效表達體系方法由genebank數據庫檢索蛇毒鋸鱗蝰素（ echistatin ）的氨基酸序列，結合大腸桿菌蛋白質合成體系對氨基酸密碼子使用的偏愛性，設計了echistatin編碼基因，體外人工合成編碼基因dna片段，通過適當的限制性內切酶位點插入表達載體pbv220 ，分別構建了echistatin的單拷貝表達克隆、雙拷貝串聯表達克隆；進一步通過pcr技術構建echistatin的融合表達基因克隆。
  9. Streptomyces low copy number plasmid scp2 * is also employed for the cloning and recovery of large dna fragment from streptomyces. as scp2 * can accommodate large dna insertions and is efficiently transmissible among its permissive hosts such as s. lividansbut seemed to be unable to replicates in streptomyces sp. fr - 008, it might be a suitable vector for the cloning of the entire fr - 008 pks gene cluster through gene replacement followed by conjugation with 5

    Scp2 *可以在變鉛青鏈黴菌等許可宿主內復制並在許可宿主間有效轉移，但似乎不能夠在鏈黴菌fr - 008中復制，因此可能適合於在鏈黴菌fr - 008中通過進行基因置換及隨后的鏈黴菌fr - 008和變鉛青鏈黴菌間的接合過程來克隆完整的fr - 008生物合成基因簇。
  10. I have used low copy pbin19 and single copy pmw755i5j binary vectors as backbone plasmids, to create a gene targeting insertion vector designated gfp tnos. after agro - infiltration into transgenic nicotiana benthamiana 16c, progeny were analyzed genetically for phenotypic changes, sirna accumulation, and dna methylation

    採用農桿菌浸潤法（ agro - infiltration ）感染轉基因本生煙16c ，並對同源基因瞬時表達所引起的植物表型變化、小分子rna的產生、 dna甲基化程度、以及相關性狀在後代中的遺傳情況進行了檢查。
  11. Carriers possessing only one copy of this gene are much more common ( around one in 25 ) than individuals with the disease ( around one in 2, 300 )

    只有當復制的有缺陷基因來自父母雙方時，才會引發疾病。所以，只攜帶來自父方或母方一方缺陷基因的正常人更為常見（機率為1 ： 25 ） ，而會患遺傳病人之機率僅為1 ： 2300 。
  12. They can pass on the gene to their children and have no symptoms of disease themselves, and the single copy of the founder mutation gives the carrier an advantage in the struggle for survival

    攜帶者能將突變基因遺傳給他們的孩子，本人卻不會表現出任何疾病癥狀，但是這一個創始者突變基因，卻可以讓他們在遇到困境時擁有生存優勢。
  13. Then, we transformed those two genes into tomato and tobacco plants via agrobacterium tumefaciens. after verified by antibiotic resistance, reporter gene examination, southern blot detection, and genetic segregation analysis, we obtained 3 and 7 transgenic tobacco plants with one copy of rbcs 3a - gus or rbcs 3c - gus gene, respectively. further, we established two suspension - cultured cell lines using above mentioned two kinds of transgenic tobacco plants

    對得到的再生煙草植株分別進行了報告基因表達水平檢測、 southern - blot鑒定以及t _ 0代轉基因種子遺傳分離規律分析，分別得到了單拷貝的穩定表達番茄rbcs3a - gusa 、 rbcs3c - gusa和35s - gusa基因的轉基因煙草3株、 7株和1株，同時還得到單拷貝的只轉化gusa基因的陰性對照轉基因煙草3株。
  14. The hplap induced from the clone with two copies of hplap gene had higher enzyme activity. the clone with more or less copy was unfavourable to the hplap expression in p. pastoris

    對部分克隆進行誘導表達發現只有2拷貝轉化子表達產物hplap活性最高，過高過低拷貝數均不利丁hplap的表達。
  15. Southern analysis showed that the cspds gene was a single copy in saffron

    Southern印跡表明該基因在番紅花柱頭中以單拷貝形式存在。
  16. Although some diseases occur when both copies of a gene are mutated, mutation of just one copy of certain tumour - suppressor genes promotes tumorigenesis

    雖然，一些疾病發生在當一個基因的兩個副本均發生突變或者只是某個腫瘤抑制基因的一個副本發生的突變促進了腫瘤的發生發展。
  17. Construction and expression of tandem multi - copy gene edcoding ca ( 1 - 8 ) me ( 1 - 10 ) hybrid fused acidic peptide to improve antimicrobial peptide ' s expression, a novel mass - production method is proposed. it is based on the neutralization of the positive charges of antimicrobial peptide by fusing to an acidic peptide to avoid the lethal effect of the expressed peptide on the host cells

    為提高抗菌肽的表達，設計在抗菌肽基因的n端融合一段編碼酸性短肽的片段作為對抗菌肽前體的模擬，以減輕表達產物對宿主的毒性；同時通過含有酶切位點的接頭將該融合肽基因以同向串連的方式連接成多拷貝基因。
  18. Osdd2 gene existed as a single copy gene in the rice genome based on the result of southern blot analysis. we took a pcr method to clone the cdna of osdd2 gene

    使用克隆的邊端插入序列為探針對水稻（中花11品種）進行southern雜交分析，表明osdd2基因在水稻基因組中是以單拷貝存在。
  19. Based on the blast analysis and other studies, osddl mutant was a multi - copy - insertion mutant, and one of the insertion sites was in an nptii like transposase gene, whereas osdd2, a single - copy - insertion mutant was disrupted at a gene encoded wrky transcript factor

    Osdd1突變體可能是多拷貝插入，其中一個插入到nptii轉座酶類似基因。 osdd2突變體為單拷貝、插入到一個wrky類轉錄因子基因5翻譯起始區附近。
  20. In this study the heat output also suggested there was obvious relation between the gene copy number and heat output. the heat output rate of different strains with various copy number is bmb304gfp ) bmb315gfp

    研究含質粒拷貝數目分別為4 、 15的gfp菌株bmb304gfp 、 bmb315gfp的熱動力學變化，發現質粒拷貝數目高的菌株向外釋放的代謝熱少，反之亦然。
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