gene hybridization 中文意思是什麼
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A genetic transformation model for the brown seaweed undaria pinnatifida has been primarily set up by using micro - particle bombardment as the method, female or male gametophytes as the gene recipients, hybridization as the regeneration route and chloramphenicol, hygromycin or basta as selective reagents
本文從轉化受體、轉化方法、報告基因、再生途徑、篩選方法等方面對裙帶菜的遺傳轉化進行了研究。首先，分離並建立了裙帶菜雌雄配子體的無性繁殖系，進行了裙帶菜的不同再生途徑的研究。 -
That cell hybridization can be used to dissect regulatory mechanisms controlling gene expression in eukaryotic cells.
雜交細胞能被應用於剖析真核細胞中控制基因表現的調節機理。 -
A homolog of ga sbeta - hydroxylase gene expression pattern was examined. hybridization signals of this gene could be detected at day 5 and however, they appear again after day 10 when explants cultured with hormones. it has the similar pattern when the explants cultured without hormones or uncultured
風信子ga羥基化酶類似基回在外植體表達，在含有外源撒素條件下培養的前5天受到激素的抑制而幾乎不表達，第10天開始在花分生組織中表達。 -
Introgression ( introgressive hybridization ) the introduction of genetic material from one gene pool to another by hybridization and subsequent back - crossing to one or other of the parents
漸滲現象（漸滲雜交） ：是通過雜交或其後代與某個親本回交，遺傳物質從一個基因庫被引入另一個基因庫的現象。 -
1. because the taxonomic division is rather complex and has been much disputed and revised, in this part, we will review the classification and phylogeny of families, subfamilies and tribes of anseriformes based on morphology, ethology, osteology, mitochondrial and nuclear dna restriction fragment length polymorphism, single - copy nuclear dna hybridization and the sequences of mitochondrial gene analysis referring to the different definition, classification and phylogenetic relationships of the families, subfamilies and tribes of anseriformes. the controversial questions and deficiency in the systematic studies of anseriformes were pointed out
具體包括以下幾個部分： 1 、針對雁形目鳥類異常復雜的分類狀況及分類上存在的爭議，根據雁形目鳥類的形態學、行為學、骨骼學、角蛋白、線粒體與核dna酶切片段長度多態、單拷貝核dna - dna雜交及線粒體基因dna序列分析等方面的研究，對雁形目鳥類分類中科、亞科和族的劃分及其相互間的系統發生關系進行綜述，分析系統學研究中存在的不足，提出了雁形目鳥類分類中急需解決的問題。 -
The aim of this study was to examine ppar5 expression in rat and mouse uterus during early pregnancy, pseudopregnancy, delayed implanation, artificial decidualization and regulation by steroid hormone treatment by in situ hybridization and inununohistochemisny the expression of ppar gene in preimplanation embryo was also determined by rt - pcr
本實驗以大鼠和小鼠為材料，利用原位雜交和免疫組化方法檢測了ppar基因在早期妊娠子宮中的表達，並利用假孕、延遲著床、人工蛻膜化及激素處理等模型研究ppar基因在子宮中的表達與調節。 -
To dress the question if other virulence gene were present in this kind of strains, 152 of 436 irp2 - hybridized strains were re - confirmed and selected for this study. the virulence genes or putative virulence genes detected by pcr or hybridization include heat stable toxin ( st ) & heat labile toxin ( lt ) for enterotoxigenic e. coli ( etec ), invasive plasmid antigen b ( ipab ) for enteroinvasive e. coli ( eiec ), epec adherence factor ( eaf ), epec secretion protein c ( espc ) for enteropathogenic e. coli ( epec ), hemolysin ( hlya ) and shiga toxins ( sltl and slt2 ) for enterohaemorrhagic e. coli ( ehec ) and eaggec probe for entero - aggregative e. coli ( eaggec ). the prra and yc73 genes of pathogenicity associated island ( pai ) of urepathogenic e. coli ( upec ) and " o " island 28 ( rtx 615 ) gene was also detected, the later was a newly discovered putative pathogenicity island in e. coli o157 : h7
為探討攜帶小腸結腸炎耶爾森氏菌的hpi毒力島的大腸桿菌是否具有其他已知的毒力基因，選取82株由原位雜交和pcr方法初篩irp2陽性的大腸桿菌菌株，進行在致瀉性大腸桿菌的25個毒力基因的檢測，包括腸產毒性大腸桿菌的熱穩定毒素st和熱不穩定毒素lt ，腸侵襲性大腸桿菌的侵襲蛋白b基因ipab ，腸致病性大腸桿菌的eaf 、 espc基因，腸出血性大腸桿菌的溶血素hly 、志賀毒素1 （ slt1 ） 、志賀毒素2 （ slt2 ）基因，腸集聚性大腸桿菌的eaggec探針，以及在泌尿道致病性大腸桿菌和o157 ： h7大腸桿菌中新發現的毒力島基因。 -
Lastly by using the technique of dot blot hybridization, the genome dna of chlamydia was detected with the probe of momp gene labeled with dig - 11 - dutp by using the way of random primer. the results showed the degree of sensitivity of the probe was 10 pg and other pathogens could not be detected by this probe. by comparing the diagnostic ways of nucleotide probe and fc, the technique of nucleotide probe were proved to have high sensitivity and speci fi city
最後，用地高辛隨機引物法標記成momp基因核酸探針，斑點雜交檢測衣原體基因組dna ，靈敏度可達10pg ，且不能檢出其它病原體的核酸。將核酸探針法與補體結合反應法對衣原體感染的診斷進行比較，初步證明該探針具有較高的敏感性與較強的特異性。 -
The engineering bacterium which carried bcih i - chi and i - glu cdna was pcg - ii. two methods of agrobacterium - mediated and gene gun were used to transformate long ya lillium. the results of pcr analysis and southern dot blotting hybridization demonstrated that the chi a nd glu cdna have been intergrated into host genome. at the same time ; compared agrabactenum - mediated method with gene gun method, the transformation frequency of the former was 16. 7 %, while the latter was 50 %, so gene gun transformation method was suitable for long ya liiliwn
用攜帶有幾丁質酶基因和- 1 、 3葡聚糖酶基因的工程菌，通過農桿菌介導法和基因槍轉化法轉化龍牙百合，經pcr和點雜交檢測證明外源基因已經整合到植物染色體中。同時對農桿菌介導法和基因槍法進行比較，發現農桿菌介導法的轉化率為16 . 7 ，基因槍法的轉化率為50 ，因此可能基因槍轉化法更適于龍牙百合的遺傳轉化。 -
Hybridization bands were detected by southern blot analysis using lea3 gene probe labeled by digoxigenin the result showed that foreign lea3 gene integrated into strawberry genome
2 。對點雜交為陽性的植株進行southernblot分析，轉化植株檢測到了雜交帶，除預期的1 -
The env protein deduced from env gene encodes the hydrophilic surface protein ( su ) and the hydrophobic transmembrane domain ( tm ) that determine the specific interaction between virus particles and cell surface receptors during retroviral entry. the su of retroviruses is a highly variable genetic element, containing receptor binding sites and major antigenic determinants. exjsrv - specific dna probes were derived. by using these dna probes in tissue hybridization. we successfully identified jsrv mrna expression and proviruses dna in sheep lung tissues infected with jsrv and control group has no postive signals, validating the use of exogenous virus - specific dna probes in the analysis of oncogenic proviral integration sites and identification of integrated exogenous proviral sequences
用地高辛隨機引物法標記exjsrv特異的env片段，制備探針，原位雜交檢測spa肺組織中的rna及前病毒dna ，結果表明spa患羊肺組織內有jsrvenv基因mrna的表達，同時也檢測到了前病毒dna ，而相應的陰性對照卻無陽性信號，證實外源性病毒特異的dna探針在致瘤性前病毒的整合位點和整合的外源性前病毒的檢測中具有可信度。 -
Was multiplied and the tk gene was cloned. the cloned tk gene was retrieved by proper restrictive hemodynamics. the retrieved tk gene was labeled by digoxin according to the kit of labeling and detection of digoxin. then, the specificity and sensitivity of tk gene probe were detected with dot blot hybridization. the sequence of tk gene of nm98a strain was analysed. the result of the analysis of tk gene ' s sequence confirmed that autoploidy between tk gene of nm - 98a strain and issued strain was 99. 7 %
本研究中首次對iltv - nm98a株的tk基因進行了克隆和序列分析，結果表明： iltv - nm98a株tk基因的核苷酸序列與已發表的iltvtk基因的核苷酸序列具有高度的同源性，兩者之間僅相差4個核苷酸，同源性高達99 . 7 ，從而證實了iltvtk基因是高度保守的，為iltvtk基因核酸探針的制備提供了有力的依據。 -
Study of gene expression of p16 with in situ hybridization technique
16基因在小鼠胚胎發育過程中的表達 -
With strong specificity and easy to manipulation, this simple, inexpensive, rapid molecular beacon hybridization technique permits visual monitoring of gene point mutation and snp, which shows better advantage than pcr - sscp. 2
Mb雜交技術特異性強，簡單快捷，能進行可視性檢測，完全適用於點突變和snp的研究，技術特點明顯優于pcr sscp 。 -
By using single strand conformation polymorphism analysis of polymerase chain reaction ( pcr - sscp ), the frequency of codon 54 mutant allele of mbl structural gene in the population of hans was investigated. 3. the molecular beacon ( mb ) hybridization technique with visual monitoring was established
建立聚合酶鏈反應單鏈構象多態性分析inglestrandconforma tlonpolymorphlsmanalysisofpol仰erasechainreactionproduction ， pcr sscp ）方法，初步調查漢族人群mbl基因ggc54gac突變情況。 -
705bp dna fragment of mxnrampl gene and full cdna of mxlrtl gene which were related to resist iron stress were cloned by using malus xiaojinensis cheng et jiang - the first iron - efficient genotype in the genus malus in the world as material. ( 1 ) using fragment of nramp gene from wheat and fe ( ll ) - transporter gene fragment of maize ( zmlrt ~ ) as probes, we analysed these genes by blotting hybridization technique in malus xiaojinensis cheng et jiang
本實驗以中國農業大學園藝植物研究所篩選到的一個蘋果鐵高效基因型? ?小金海棠（ malusxiaojinensischengetjiang ）為試材，分別克隆了小金海棠的抗缺鐵相關基因mxnramp1基因的752bp基因組dna片段和fe （ ） -轉運蛋白基因（ mxirt1 ）的cdna全長，為深入探討小金海棠抗缺鐵的分子機理奠定了基礎。 -
In previous study, janghong have succeeded cloning 24 ests of mouse testis spermatogenic cell apoptosis - related gene by creating mouse cryptorchidism model and making use of suppression subtractive hybridization, and registered in genbank
在前期的研究工作中，本室姜宏等通過建立小鼠隱睪模型，運用抑制消減雜交法（ suppressionsubtractivehybridization ， ssh ）克隆出24個小鼠睪丸生精細胞凋亡相關基因的est ，並在genbank中登錄。 -
As heterologous probe and subsequently show to code for desired enzymatic activity. after a serial of subcloning coupled with southern hybridization and enzymatic activity assay, the functional s. griseus atcc14811 cholesterol oxidase gene ( chog ) was localized onto 2. 3kb ecori - sall fragment
對phz1140和phz1141進行bamh及bgl的酶譜分析及與choa探針的雜交，將膽同醇氧化酶基因初步定位在8 . 8kbbamh和9 . 9kbbgl片段上。 -
After the screening, the expression and identity of the gene for nadp - malic enzyme in leaves of aloe vera l. under salt stress had been done. methods : construction and screening of the subtractive library : to construct a cdna subtractive library of aloe vera l. under salt stress, the leaves of aloe vera l were removed from the seedlings which were treated with 300 mm nacl. suppression subtractive hybridization ( ssh ) was carried out and a subtractive library was constructed
鹽脅迫下庫拉索蘆薈葉子中nadp -蘋果酸酶基因的表達鑒定：為了檢測蘆薈鹽脅迫下庫拉索蘆薈cdna消減又庫的構建篩選及nadp一蘋果酸酶基因的鹽誘導表達中nadp一蘋果酸酶基因『刀叻心）的表達和nadp一蘋果酸酶（ nadp一ma1ieenzyme ）蛋白的積累是否受著高鹽的誘導，我們利用耐鹽品種庫拉索蘆薈（ a了口。 -
Therefore, the determination of seb is very important for food hygienic analysis as well as for clinical analysis. nucleic acid hybridization technique is one of the widely - used methods in molecular biology and gene technology. the present work has developed piezoelectric biosensors used in the detection of seb dna by tacking the piezoelectric quarts crystal as a sensitive component while synthetic oligonucleotide probe as recognize molecule
其中b型葡萄球菌腸毒素（ seb ）是一種通常條件下更穩定，毒性最強的毒素，而核酸雜交技術則是分子生物學和基因工程中最常用和最基本的方法之一，因此本論文以該毒素的產毒基因為檢測對象，以壓電石英晶體為敏感元件，以合成的寡核苷酸探針為識別分子，構建了用於seb基因檢測的壓電生物傳感器。
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