gene transfer vector 中文意思是什麼

gene transfer vector 解釋
基因轉移載體
  • gene : n. 【生物學】基因。 dominant gene顯性基因。
  • transfer : n 1 移轉，轉送；調職；調任[轉學]證書；變換。2 （財產；權利等的）轉讓，讓與（證書），移轉，授受；...
  • vector : n 1 【數學】向量，矢量，動徑。2 【航空】飛機航線；航向指示。3 【天文學】幅，矢徑。4 【生物學】帶...
  1. Envelope gene gp85 of imc10200 subgroup j avian leukosis virus was cloned and expressed in the present study. the sequence encoding the gp85 domain of imc 10200 alv - j was amplified from pgem - imc2. 2 vector, which contains env gene of alv - j imc 10200 strain, and cloned into transfer vector pfast bacl

    為深入探討alv - j的亞群特性，本研究利用alv - jgp85基因兩側的序列片段為引，物從正常spf蛋雞、商品肉雞和df1細胞基因組中完整地擴增了內源性類alv - jgp85基因。
  2. Construction of recombinant fowlpox virus coexpressing aiv ha and ndv f. for the construction of transfer vector pfgs11haf, aiv ha gene of f strain in puc18ha and ndv f gene of f48e8 strain in puc19f were removed and inserted into pfgs11. recombinant fowlpox viruses ( rfpv ) coexpressing aiv ha gene and ndv f gene were constructed by using different promoters of ps and pe / l. recombinant rfpvs were derived by dosper liposome - mediated transfection with the two transfer vectors on chicken embryo fibroblast ( cef ) monolayer cultures which were infected by wild type fpv chinese vaccine strain 282e4 3 - 4 hours earlier

    Puc18ha和sk質粒同時經hind 、 kpn酶切后連接得中間質粒skha ；將質粒skha用bamhi酶切回收ha基因插入到插入載體pfgs11中的bamhi位點，通過酶切鑒定獲得了pfgs11ha ；將含ndvf基因的質粒puc19f用hind 、 sal酶切經klenow酶補平插入到經sma酶切后的skifn中pe / l啟動子下獲得中間質粒skf ，再將質粒skf和puc18質粒先分別用ecor 、 xho酶切klenow補平，后再共同用sac酶切連接得puc18pelf ， sal酶切回收pe / l - f基因盒插入到pfgs11ha的sal位點，通過酶切鑒定獲得了pe / l - f與ps - ha同向的表達載體pfgs11haf 。
  3. Vp3 gene of hl isolate of goose parvovirus derived from recombinant piasmid pproex htb - vp3 was subcloned into ecorl site of psy538, and the reporter gene lacz with promoter pll was cloned into smai site of recombinant piasmid. both vp3 gene and lacz gene were cloned into noti site of psy681, recombinan fpv transfer vector containing vp3 gene of gpv was obtained. the result is basis of construction of recombinant fpv expressing vp3 gene of gpv and gpv genetic engineering vaccine

    本研究另從含有gpvh1分離株主要結構蛋白vp3基因的重組質粒pproexhtb - vp3中切取gpvh1株vp3基因片段，將其亞克隆于psy538的ecori位點，然後將帶有痘苗病毒啟動子p11的lacz報告基因平端克隆于上述重組子的smai位點，再用noti切下同時含有vp3基因和lacz報告基因的片段，再亞克隆于psy681的noti位點，構建出含有vp3基因的重組禽痘病毒轉移載體，為構建表達vp3基因的重組禽痘病毒從而制備gpv基因工程疫苗奠定了基礎。
  4. The transfer vector pbdtk - uni can be used for the construction of recombinant prv expressing foreign gene ( s ). postgraduate : tianzhijun specialty : preventive veterinary science supervisors : prof. li yijing prof. tong guangzhi

    以上結果表明所構建的具有自主知識產權的通用prv轉移載體pbdtk - uni是成功的，為利用該載體構建偽狂犬病病毒二價基因工程疫苗提供了技術平臺。
  5. Two types of repeat sequence, a 15 - amino - acid ( eelcaqlcstppppi ) with 2 repeats and a 6 - amino - acid ( ppictp ) with 4 repeats, were firstly reported. 2. the characterization of meq gene product and its expression within the cells a recombinant baculovirus transfer vector pblubac4 - meq was constructed by cloning meq gene of marek ' s disease virus ( mdv ) ga strain into the baculovirus transfer vector pbluebac4 under the polyhedrin promoter

    此外，研究還發現了meq基因的兩類有趣的重復結構，其中一類是含15個氨基酸殘基（ eelcaqlcstppppi ）的結構，有2個重復，另一類是含6個氨基酸殘基（ ppictp ）的結構，共有4個重復，它們全部分佈在meq蛋白c -端的轉錄激活域內。
  6. 3. using clamp technique, ex vivo gene transfer into liver graft was performed during cold preservation via perfusion of the portal vein with 5ml ringer ' s solution containing replication - defective adenovirus vector adhuctla4 - ig

    供肝冷保存時，採用血管夾技術lamptechnique ）經門靜脈灌注攜帶融合基因hllctla4dg的重組腺病毒，于術后3天、 7天能定性檢測到hllctla4ig在受體外劃血卜1 。
  7. To obtain large amounts of appa phytase, the appa gene was subcloned into the prokaryotic expression vector pet - 28a ( + ) and baculovirus transfer vector pvl - 1393 under the control of the lac and polyhedrin promoter, respectively. the appa phytase was overexpressed in e. coli strain bl21 induced by lactose

    為了大量表達appa植酸酶，我們將appa基因分別克隆至原核表達載體pet - 28a （ + ）和桿狀病毒轉移載體pvl - 1393中，將其分別置於lac和polyhedrin啟動子控制之下。
  8. In order to get the soluble recombinant eo protein and inspect the protein expression status convinently, the egfp and eo gene were ligated into baculovirus transfer vector. with the co - transfecting sf9 cells of baculovirus recombinant transfer vector and linearized viral dna, and plaque purification in the posttransfection procedure, the pure recombinant baculovirus were harvested, which infected the sf9 cells for amplifying to generate a p - l stock. in the meantime, the fluorescence microscopy detection indicated expressed egfp protein to confirm the heterogenous protein expression of recombinant baculovirus. the pi - stock from a pure plaque was used to generate a high liter p - 2 stock, which was determined in liter as 1. 14 107pfu / ml by performing a plaque assay. when a volume of p - 2 stock infected the sf9 cells with moi 5 - 10 for expression, the strong fluorescence was obeserved on the day 3 of postinfection

    此外，為了得到可溶性重組eo蛋白並便於觀察重組蛋白的表達情況，我們將egfp基因與eo基因相連插入昆蟲桿狀病毒轉移載體中，與線性桿狀病毒dna共轉染sf9細胞后通過噬斑純化得到純的重組桿狀病毒，將其感染sf9細胞制備p1種子液，同時用熒光顯微鏡觀察綠色熒光蛋白的表達情況剔除表達效果差的重組桿狀病毒。再用p1種子液感染sf9細胞制備高效價的p2種子液。通過病毒液的梯度稀釋和噬斑測定，確定p2種子液的病毒滴度達1 . 14 10 ~ 7pfu ml 。
  9. In this study, a 1. 7kb kpni fragment and a lacz gene expression cassette carrying the e. coli lacz gene under the control of sv40 promoter were inserted into the transfer vector pbdtk - uni ( a 277bp acci - accl fragment in the tk gene was deleted ). the new transfer vector was called puni - lacz. the transfer vector puni - lacz and purified genomic dna of strain bartha - k61 were used to cotransfect vero cells using lipofectin transfection procedure

    本研究以呈ge ~ -表型的經典弱毒疫苗bartha - k61株為親本株，在通用prv轉移載體pbdtk - uni的基礎上，在其多克隆位點中插入由sv40早期啟動子控制下的lacz基因表達盒，同時將下游同源臂增加了一個1 . 7kb的kpni片段，使上下游同源臂的長度都超過了1kb ，構建了一個新的轉移載體puni - lacz 。
  10. The recombinant pcr technic was used to introduce a linking peptide klgggg to the site between scfv single chain form of the monoclonal antibody sz - 51 specific for the glycoprotein gmp140 on activated platelet membrane and uk32 low molecular weight form of pro - urokinase, to make the scfv - linker - uk32 chimeric gene. this gene was cloned into the transfer vector pbacpak9, and cotransfected with bacpak6 bsu36i digest into sf 9 cells. the fusion protein was secreted into the medium. in the fifth day after the cotransfection, the supernatant of the medium showed 107 iu ml fibrinolytic activity, higher than 25 iu ml fibrinolytic activity of scfv - uk32. elisa showed that the supernatant had the binding activity to activated platelet. wastern blotting also indicated that the supernatant could bind to the monoclonal antibody of urokinase b chain

    為了提高重組導向溶栓分子scfv - uk32的溶纖活性，通過重組pcr方法在編碼scfv與uk32的堿基之間引入編碼klgggg連接肽的堿基序列，並克隆到轉移載體pbacpak9上，通過與線性病毒dna bacpak6 bsu36i digest共轉染到昆蟲細胞sf 9內，進行表達。表達產物分泌到上清中，共轉染后第5d天用纖維平板法測得sf 9細胞上清溶纖活性達到107 iu ml ，比未引入連接肽的scfv - uk32的表達活性25 iu ml高。
  11. Results : the retroviral vector containing vegf gene was constructed successfully. immuohistochemistry showed that it expressed vegf protein in bmsc plasma ( brown staining ) and the experimental study confirmed that its mvc was higher than that in control group ( p < 0. 01 ). conclusion : gene transfer technology mediated by retroviral vector

    結果:成功構建vegf逆轉錄病毒表達載體，免疫組化方法顯示經感染的bmsc胞質中有陽性棕色顆粒出現，且動物實驗顯示新生血管計數明顯高於對照組（ p < 0 . 01 ） 。
  12. The gd and ge gene was subcloned into puc18, resulting in pugdge. the fragment from pcdnas. 1 - including hcmv promoter / enhancer, mcs and neomycin resistance gene was inserted into the bamhi and bsteii restrication sites of pugdge, resulting in the universal transfer vector pgd - m - ge. the universal transfer vector pgd - m - ge has deleted the gi gene and 363bp in the 5 ' end of the ge orf of prv. there were 11 restrication sites for insertion of the foreign gene. the upstream and downstream flanking sequences were up to 1. 25kb and 1. 42kb. it will be useful for developing the recombinant prv expressing foreign gene ( s )

    將gd 、 ge基因連接于質粒puc18獲得pugdge ，缺失質粒pugdge的bamh和bste位點間391bp的片段。在此缺失位置插入來自質粒pcdna3 . 1 -的一偽狂犬病病毒gd 、 ge 、 tk基因的克隆與通用轉移載體的構建段含hcmv啟動子。多克隆位點和neo報告基因的片段，構建了通用轉移載體ppd m pe 。
  13. Dna sequence analysis showed that the cloned nk gene was highly homologous to the sequence reported by nakamura. the deduced amino acid sequence also had the conserved sequences ( serine 221, histidine 64, and aspartic acid 32 ) which was essential for the catalytic center of serine proteases. the nk gene was then cloned into the transfer vector pvl1393

    本實驗以b . subtilis （ natto ）基因組dna為模板擴增了nk基因，測序結果顯示與nakamura報道的納豆激酶基因高度同源，其氨基酸序列也含有絲氨酸蛋白酶的活性保守中心（ serine221 ， histidine64 ， asparticacid32 ） 。
  14. Fusion gene by pcr was inserted into bombyx mori baculovirus transfer vector pbacpak. 8 and contransfected with lineared dna of bm - bacpak6 virus into bmn cells. the homologous recombination occurred inside the cells, and the recombinant virus bacpak - 6aa - hgm - csf was expressed, as identified by pcr and southern hybridization. the bmn cells and the fifth instars were infected by the recombinant virus bacpak - 6aa - hgm - csf

    本研究首先通過pcr將家蠶桿狀病毒多角體蛋白起始密碼子后的18個堿基引入到hgm - csf基因的5 』端之前，然後將融合基因重組與家蠶桿狀病毒轉移載體pbacpak8中，獲得重組轉移載體pbacpak8 - 6aa - hgm - csf ，並與線性化bm - bacpak6dna共轉染家蠶細胞株，獲得重組病毒bacpak - 6aa - hgm - csf 。
  15. Then we conducted in vitro gene transfer, transfecting p388d1 with recombinant eukaryotic vector and infecting hela with recombinant adenoviruses respectively, in order to observe inhibition of constitutive or inducible cii ta gene expression by introduced complementary antisense rna of c iita and consequent do wnregulation of class ii mhc molecules expression

    結果顯示：含反義c ta的真核表達載體轉染組成型mhc -類分子表達p388d1細胞株，經抗生素抗性篩選后表達mhc -類分子的細胞陽性率比對照組下降65
  16. Additionally, baculovirus has been a new gene transfer vector used in gene therapy due to its advantages of the extremely high expression of foreign genes and being non - reproduce vector

    另外，桿狀病毒是非復制型載體，能高效表達目的蛋白，其作為基因轉移載體在基因治療中乙顯示出良好的應用前景。
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