gene-library 中文意思是什麼
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Screening target ' gene of artemisinin antimalarials using drug - western from cdna expressing library : 12 b - deoxoartemisinyl - ( 4 ' - oxyacetic acid ) phenyl ether was linked to bsa by using of edc cross ! inker and the product acted as drug - probe
對重組pmd一18一t克隆載體及pqe一30表達載體雙酶切，提取tctp基因和pqe一30空載體並使二者重組，然後轉化m15 ，挑取陽 -
Even so, by truncating hbv pres gene, we finally obtained some useful " " bailors ", either nontoxic or self - activating, and used them to fish dna fragments of hbv pres interacting protein ( s ) from an ad vector constructed human embryonic cdna library
我們通過第回軍巨大學碩士學位論文對pres基因分段截短的方法，獲得了對酵母細胞即無毒性作用，又沒有自激活作用的「誘餌」 ，通過它在酵母雙雜交系統中篩選構建於ad載體的人胎肝。 -
In this study, we at first aimed at obtaining the gene encoding the specific ige antibody related proteins by immunoscreening the schistosoma juponi cum adult worm cdna library with the pooled high - titer ige antisera from the individuals living in the schistosome epidemic regions
J測定。 jnij序結果顯示，該插入片段為1200hp ，第一開讀框長507hp ，編碼169個氨基酸殘基，理論分子量為19 3kdi 。 -
The construction and screening of cdna expression library of oncosphere of taenia solium and cloning of immunogen gene
文庫的構建及免疫原基因的篩選與克隆 -
This sequence emergences fourteen times from 1000 ests library indicts that it is a middle affluently gene in cdna library. the cdna of 634 basepairs contains an open reading frame of 339 nucleotides encoding a novel nonspecific lipid transfer protein. the first 23 amino acids constitute the putative signal peptide, characteristic for targeting to the secretory pathway
測得th - nsltp序列全長為634bp ，含有一個非特異性脂轉移蛋白與植物耐逆性的相關性研究編碼112個氨基酸的閱讀框架， n端的23個氨基酸組成一段信號肽序列，表明它可能和分泌有關。 -
We have identified a nucleotide sequence from ests ( expressed sepuence tagged ) acquired fromzap - cdna library of thellungiella salsuginea treated with 200mmol. l - 1 nacl and analyzed its nucleotide sequence characterization, gene organization, and differential expression under salt stress
本實驗從200mmol . l ~ （ - 1 ） nacl處理的小鹽芥地上部分構建的zap - cdna文庫中克隆了編碼硫氧還蛋白的基因序列。 -
Part 1 : identification of a novel gene, tsarg2, and its sequence character cloning new apoptosis - related novel gene is a key to further understanding of apoptosis mechanism and the biological process of germ cell, and it is of momentous significance on clarifying physiology and pathology process of spermatogenesis. to rapidly attain human novel gene full - length cdna sequence, the gene - specific primers and the vector - specific primers have been designed for successful performing nested pcr and draft human genome searching to rapidly identify the tsarg2 ( genebank accession number ay040204 ) 5 " end from a human testis cdna library by using a cdna fragment ( genebank accession number be644542 ) as a electronic probe, which was significantly changed in cryptorchidism and represents a novel gene. furthermore, a mouse homologue of this gene was identified ( genebank accession number af395083 ) by lab on - line
本研究分為三個部分，其主要實驗方法及實驗結果如下：第一章tsarg2基因的克隆與序列分析從已獲得的在隱睪和正常睪丸對照中表達量有明顯差異的est片段（ be644542 ）入手，設計了基因特異性引物和載體特異性引物進行巢式pcr擴增，結合人類基因組草圖搜索法，從睪丸cdna文庫中快速分離出人類睪丸凋亡相關基因的5末端而獲得全長cdna ， genbank登錄號為ay040204 ，同時應用生物信息學的方法克隆了該基因在小鼠中的同源基因， genbank登錄號為af395083 。 -
A gene library of psedomonas fluorescens g2 was constructed in the cosmid vector pla2917 using e. coli jm109 as the host strain. two recombinants, pgr3 and pgr7, which can confer glyphosate resistance ofe. coli jm109 were identified from the selective medium containing 10mm glyphosate
以粘粒pla2917為載體、大腸桿菌jm109為受體菌構建熒光假單胞菌g2的基因組文庫，在含有10mm草甘膦的固體選擇培養基上篩選出兩個耐受克隆pgr3和pgr7 ，插入片段分別為7kb和11kb 。 -
Construction of araneus ventricosus major ampullate gland cdna library and screening spider silk gene
文庫構建和絲蛋白基因篩選 -
Nies - 90 microcystin producing algae strain genome library construction and one isolated gene analysis
構建用於相關基因篩選的微囊藻產毒株基因組文庫 -
The chloroplast shsp gene was screened from the cdna library of tomato flower by pcr strategy and confirmed by sequencing. but difference was found at 3 bases of the sequence from the reported in genbank. then, an integrated vector prok ii of the chloroplast shsp gene and nptii gene ( a kanamycin resistant gene ) with camv35s promoter was constructed and introduced into tomato mediated by agrobacterium tumefaciens lba4404. transgenic tomato were screened by their ability of growing on media containing kanamycin
本實驗採用pcr方法從番茄花cdna文庫中克隆到葉綠體shsp基因，經測序證實與genbank中已發表的序列在編碼區相差2個堿基，其中一個堿基導致1個氨基酸的改變。將葉綠體shsp基因定向克隆于帶有組成性表達啟動子camv35s的植物表達載體prok中，凍融法轉化農桿菌lba4404 ，利用葉圓盤法對番茄進行ti質粒介導的遺傳轉化。 -
The cdna library of major ampullate glands of araneus ventricousus was prepared successfully for the first time by using puc18 vector in the world. two genes encoding spider silk protein and one gene encoding no - silk protein were cloned by means of randomly picking colony ( bird gun )
本研究在國內外首次構建了大腹園蛛（ araneusventricosus ）主壺腹腺（ majorampullategland ） cdna文庫，並以鳥槍法從中篩選出兩個蛛絲蛋白和一個非絲蛋白cdna新基因。 -
Gene cloning differential fragment gha27 was produced by cdna - aflp method employed to compare the gene expression in developing anthers between the male sterile and fertile plants of cotton dong a. a cdna clone, designated gharf ( gossypium hirsutum adp - ribosy lation factor ), was isolated from a cotton ( gossypium hirsutum l. ) cdna library using gha27 as probe. 2
棉花arf基因的克隆選取棉花洞a雄性不育株和可育株花藥cdna - aflp差別顯示的差異片段gha27 ，用地高辛標記作為探針，篩選棉花洞a花藥的cdna文庫，克隆得到了棉花arf基因的cdna全長序列，將其命名為gharf （ gossypiumhirsutumadp - ribosylationfactor ） 。 -
The gpa1 gene was obtained via pcr amplification and was cloned in the two - hybrid dna binding domain vector pgbkt7 the combinant plasmid was designated as pgbkt7 - ga. - galactosidase assay indicated that got did not have the property of self - activation. after pgbkt7 - ga was transformed to yeast pj69 - 4a, we transformed arabidopsis vegetative tissue two - hybrid cdna library plasmids to yeast pj69 - 4a containing pgbkt7 - ga
通過pcr擴增得到gpa1基因並將其克隆到雙雜交dna結合域載體pgbkt7中，得到的重組質粒命名為pgbkt7 - g ， ?半乳糖苷酶活性鑒定表明g不具有自激活特性，將其轉化到酵母菌pj69 - 4a中，再以此為受體菌轉化擬南芥綠色營養組織cdna文庫質粒。 -
After the screening, the expression and identity of the gene for nadp - malic enzyme in leaves of aloe vera l. under salt stress had been done. methods : construction and screening of the subtractive library : to construct a cdna subtractive library of aloe vera l. under salt stress, the leaves of aloe vera l were removed from the seedlings which were treated with 300 mm nacl. suppression subtractive hybridization ( ssh ) was carried out and a subtractive library was constructed
鹽脅迫下庫拉索蘆薈葉子中nadp -蘋果酸酶基因的表達鑒定：為了檢測蘆薈鹽脅迫下庫拉索蘆薈cdna消減又庫的構建篩選及nadp一蘋果酸酶基因的鹽誘導表達中nadp一蘋果酸酶基因『刀叻心）的表達和nadp一蘋果酸酶（ nadp一ma1ieenzyme ）蛋白的積累是否受著高鹽的誘導，我們利用耐鹽品種庫拉索蘆薈（ a了口。 -
In our studies, we have used the nuclear localization signal department of medical genetics and development biology 4 ( nls ) selection system to isolate a novel gene from a mouse embryonic cdna library. the full - length cdna is about 1802bp and contains an open reading frame of 1296 nucleotides. it encodes 431 amino acid
本研究工作，利用本室構建的核定位信號篩選系統從小鼠胚胎cdna文庫中克隆到一個新的全長cdna片段，分析表明在其1802bp育回軍區大學刃士學位論文的序列中含有一個長1293hp的開放閱讀框，編碼431個氨基酸。 -
Strain sa - coo by southern hybridization. a cosmid - based gene library of streptomyces griseus atcc14811 was constructed using phz1357, a streptomyces - e. coli bifunctional vector carrying two cohesive sites
為了獲得膽固醇氧化酶基因，以大腸桿菌-鏈黴菌雙功能柯斯質粒phz1357為載體，構建了灰色鏈黴菌atcc14811的基因組文庫。 -
It ' s imperative to establish the rh gene library based on han nationality corresponding primer designing and gene typing method suitable for chinese
建立以漢民族為代表的rh基因文庫，以及相上的引物設計和更適合中國人群的基因檢測方法，勢在必行。 -
It was necessary for gene library construction and transformation with enzyme ligased products. there were many factors to affect the preparation of competant cells. the affected factors of preparation of competence cells and transformation process had been studied
已知影響感受態細胞制備的因素很多，本研究從感受態細胞制備的影響因素和轉化過程對轉化效率的影響因素兩個方面進行了研究。 -
The researchers propagated individual dna fragments in the traditional way, in bacteria, to create a gene library ; they also developed a method for pulling out specific sequences from the library, providing an easy way to study any gene of interest
研究者用傳統方法擴增單獨的dna片段，在細菌中建立基因文庫；他們同時也開發出一種從文庫中選出特殊序列的新方法，為研究任何感興趣的基因提供了一種簡單途徑。
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