gene-specific 中文意思是什麼

gene-specific 解釋
基因獨特性
  • gene : n. 【生物學】基因。 dominant gene顯性基因。
  • specific : adj 1 特殊的;特有的;特定的,專門的。2 明確的,具體的。3 【生物學】種的;【細菌】專性的。4 【醫...
  1. By means of discontinuous tris - hcl buffer system and poly aery 1 amide gel electrophoresis ( page ), zymogram of 23 species of 11 genera in 4 subfamilies mirinae, orthotylinae, phylinae and deraeocorinae are gained. by specific primer pcr and sequencing, 57 cyt b gene 433bp sequences are obtained from 34 species respectively belonging to 17 genera in 5 subfamilies mirinae, orthotylinae, phylinae, deraeocorinae, bryocorinae from 64 species of 23 genera which involved in this research, and 2 outgroup species of family anthocoridae as well. 1

    首次通過特異引物擴增和基因測序的研究方法從被試的5亞科23個屬的64種盲蝽中獲得了盲蝽亞科、合墊盲蝽亞科、葉盲蝽亞科、赤爪盲蝽亞科和蕨盲蝽亞科bryocorinae5個亞科17個屬的34種盲蝽以及外群花蝽科anthocoridae2種花蝽的cytb基因序列57條。
  2. Viral rnas were extracted from virus - infected allantoic fluids using qiaamp mini - extraction kits. after reverse transcription, cdna was amplified using specific primers for each gene segment

    用qiagen的rneasyrna試劑盒提取病毒rna后,逆助興才李醫『筍脂碩聖『老『才轉錄合成cdna 。
  3. The global regulator csra of e. coli is a specific mrna - binding protein. csra negatively regulates several metabolic pathways that are induced post - exponentially, including glycogen biosynthesis, gluconeogenesis, and glycogen catabolism ; positively controls gene expression within the glycolytic pathway ; and also csra modulates the levels of enzymes that participate directly in pep metabolism

    Csra是整體調控網路的調控基因,可負調控指數生長後期誘導的一些代謝途徑,包括糖原的生物合成、糖原的分解代謝、糖異生,而對糖酵解的一些重要基因的表達則執行正調控功能, csra也調控直接參與pep代謝的三個酶的活性水平。
  4. The target gene was then subcloned into plasmid vector and induced by iptg for its expression. after that, the recombinant protein was purified and used for the identification and characterization of its immunogenicity. the effect of the induced specific ige antibody on the hepatic granuloma formation and fibrosis after challenge infection with schistosome cercariae was evaluated

    Niptg誘導表達,產物沉澱中於約45kda處顯見高合蛋白表達帶, westernblot分析表明,該蛋白帶可被篩庫血清中特異性lge抗體識別;而載體本身表達的26gst蛋白帶則否。
  5. Earlier this year, a study by gerd pfreier of beckman research institute pointed out the specific substance causing cancer in cigaret smoke, whose target was some parts of gene which, people discovered, played a prominent role in some cancers

    而今年早些時候,貝克曼研究所的格爾德?普法伊費爾所作的一項研究確切地指出了卷煙煙霧中固有的致癌物,這些致癌物襲擊的目標是一種基因的某些部分,人們已經發現,這種基因在一些癌癥中很突出。
  6. Gene cloning provides a means of purifying and propagating specific dna segments.

    基因克隆化提供了一種純化和擴增特定DNA片段的方法。
  7. Many organisms produce enzymes, termed photolyases, that specifically bind to these damage products and split the via a uv - a / blue light - dependent mechanism ( photoreactivation ), thereby reversing the damage. these two photolyase are specific for either cpds ( cpd photolyase ) or 6 - 4 products ( 6 - 4 photolyase ). a gene that expresses a protein with 6 - 4 photolyase activity in vitro, was recently cloned from high organisms ( arabidopsis thaliam, drosophila melanogaster, danio rerio, xenopus laevis and homo sapiens )

    目前已從高等生物擬南芥、鮐類、果蠅、人類和非洲爪蟾蜍屬中克隆到有( 6 - 4 )光裂合酶活性的基因,本研究從鹽生杜氏藻dunaliellasalina中克隆到( 6 - 4 )光裂合酶的基因,並將該基因在大腸桿菌中得以表達,這是首次在藻類中克隆到( 6 - 4 )光裂合酶基因,對光裂合酶的研究具有重要意義。
  8. Cloning and expression in e. coli of lactase. specific primers were designed according to the sequence of the beta - galactosidase gene from kluyveromyces lactis. klac gene was amplified by pcr and subsequently cloned

    乳糖酶基因的克隆及原核表達以乳酸克魯維斯酵母( kluyveromyceslactis )菌株k538的基因組dna為模板,設計引物,利用pcr獲得乳糖酶基因klac ,經dna測序驗證,得到克隆t1549 。
  9. All kind of oligonucleotides ( 16 sense primers, 8 antisenset primers, 128 different primer combinations in all ) were designed from the conserved motifs glpl and cfly ( distance between them is approximately 210bp ) which existed only in the type of nbs - lrr resistance gene and used as primers for scanning the genomic dna. no specific products ( which is approximately 210bp in length and obtained from genomic dna containing ht gene ) was obtained though amplified products of approximately 500 - looobp appeared in six different primer combinations

    依據nbs - lrr類r基因特有的兩個保守序列glpl和cfly (兩者相距約70個氨基酸)合成所有可能的簡並寡核苷酸引物(左引物16個,右引物8個,共組成128個引物組合)對玉米基因組dna進行擴增的結果表明:在6個引物組合中得到了擴增產物,產物長度在500 1000bp之間;沒有獲得特異性擴增產物(指僅從含ht基因材料中得到,長度約210bp的dna片斷) 。
  10. Now transgenic cell lines that express the protein encoded by ugt gene were broadly used instead of liver microsomes. the enzyme expressed in cell lines helps to realize the function of a specific gene and will provide direct information related to the isozyme interested

    『為解決上述問題,國際上人們己經廣泛採用建立各種ugt轉基因細胞系的方式試驗,在明確基因構成的倩況下直接獲得由特定基因所表達的ugt同工酶, fi什對性地研究該酶對藥物的代謝作用。
  11. Site - specific mutagenesis of murine anti - human tnf - fab gene and its expression in e. coli

    基因的定點突變及其在大腸桿菌中的表達
  12. Research on search and detection of specific endogenuous reference gene in brassica napus

    油菜內源特異參照基因尋找與檢測研究
  13. Molecular basis of neural - specific gene expression

    神經特異性基因表達的分子基礎
  14. Construction of neuron specific expression vector for human noggin gene

    基因神經細胞特異性表達載體的構建
  15. Mouse homeobox gene pem was newly isolated in 1990 ' s. its expression pattern during the murine ontogeny and in the adult tissues is unique, namely, in a stage and spatial specific manner

    Pem基因在小鼠發育過程及成體部分組織中呈時間和空間特異性表達,並在一系列腫瘤細胞株中持續表達。
  16. By race pcr, amplified products were obtained with all the gene - specific primers and the adaptor primers

    由氨基酸序列推斷的ejo基因編碼蛋白的等電點為6 18 ,分子量為28
  17. Using rt - pcr with two degenerate primes designed from conserved amino acids of cdpk kinase and autoinhibitory domains, three 618 bp cdna fragments ( 618 - 1. 618 - 2 and 618 - 3 ) were also isolated from broad bean leaves, and a partial cdna vjcpk2 lacking 5 " end was isolated from broad bean leaves with two gene - specific primers designed according to the 618 - 1 sequence

    用rt - pcr技術,以cdpks激酶區和連接區的保守氨基酸設計的一對簡並引物,從蠶豆葉片中還擴增到了3個618bp ( 618 - 1 、 618 - 2和618 - 3 )的cdna片段;用race技術,以618 - 1序列設計的一對基因特異性引物,克隆到了還缺少5 』末端的cdna片段vfcpk2 。
  18. Part 1 : identification of a novel gene, tsarg2, and its sequence character cloning new apoptosis - related novel gene is a key to further understanding of apoptosis mechanism and the biological process of germ cell, and it is of momentous significance on clarifying physiology and pathology process of spermatogenesis. to rapidly attain human novel gene full - length cdna sequence, the gene - specific primers and the vector - specific primers have been designed for successful performing nested pcr and draft human genome searching to rapidly identify the tsarg2 ( genebank accession number ay040204 ) 5 " end from a human testis cdna library by using a cdna fragment ( genebank accession number be644542 ) as a electronic probe, which was significantly changed in cryptorchidism and represents a novel gene. furthermore, a mouse homologue of this gene was identified ( genebank accession number af395083 ) by lab on - line

    本研究分為三個部分,其主要實驗方法及實驗結果如下:第一章tsarg2基因的克隆與序列分析從已獲得的在隱睪和正常睪丸對照中表達量有明顯差異的est片段( be644542 )入手,設計了基因特異性引物和載體特異性引物進行巢式pcr擴增,結合人類基因組草圖搜索法,從睪丸cdna文庫中快速分離出人類睪丸凋亡相關基因的5末端而獲得全長cdna , genbank登錄號為ay040204 ,同時應用生物信息學的方法克隆了該基因在小鼠中的同源基因, genbank登錄號為af395083 。
  19. Total rna was extracted from the second stage larve of hypoderma sp, then single chain cdna was synthesized by reverse transcription using oligo ( dt ) 18 as a primer. the hypodermin c ( hc ) and hypodermin a ( ha ) gene specific primers were devised by dnastar software

    本試驗的目的旨在進行hypodeminc ( hc )和hypodermina ( ha )基因的克隆、測序、構建重組表達載體並誘導表達,獲得重組抗原,以解決天然抗原的不足並為診斷和免疫試劑的產業化奠定基礎。
  20. A complete cdna of 1047 bp was obtained by means of 5 ' - race ( 5 ' - rapid amplification of cdna end ) techniques using gene specific primer p1a5 - 1

    Rtpcr結果證明它在苞片中強烈表達而在處于同時期的葉片中幾乎檢測不到其表達。
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