germ line cell 中文意思是什麼
germ line cell
解釋
生殖譜系細胞-
2. isolation and cloning of mouse embryonic germ cells of icr species primordial germ cells ( pgc ) were isolation from 8. 5 - 15. 5 dpc ( days post coitum ) embryos. eg cell lines with the characteristic of murine es cell line were established and continuously cultured to 6th passage
Icr小鼠eg細胞的分離克隆研究本試驗以icr品系小鼠胚胎8 . 5 15 . 5dpcpgc為材料，經傳代培養，獲得能連續而穩定傳至6代的，具有胚胎幹細胞諸多特性的eg細胞系。 -
Multi - locus dna fingerprint technique was used to check the chimerism of chimeric mouse generated by injecting es cells into blastocysts and to detect whether the chimeric mouse is a germ - line chimeras. the results indicated that : the multi - locus dna fingerprint with a new synthesized probe - jl - 02, has enough polymerism and good stability, and should be very useful to monitor the chimerism in different tissues of es cell chimeric mouse and to check whether an es cell line has the capacity to enter the germ line, especially when involving strains that can not be discerned with coat color or biochemical markers
嘗試應用多位點dna指紋技術，檢測經過胚胎幹細胞es細胞途徑所獲得的嵌合體小鼠中es細胞在各種臟器中的嵌合情況檢測es細胞在嵌合體小鼠中是否實現種系傳遞。結果表明：採用新型的人工合成的寡核苷酸多聚體探針jl - 02探針的多位點dna指紋圖譜，具有足夠的多態性和很好的穩定性。 -
Part 1 : identification of a novel gene, tsarg2, and its sequence character cloning new apoptosis - related novel gene is a key to further understanding of apoptosis mechanism and the biological process of germ cell, and it is of momentous significance on clarifying physiology and pathology process of spermatogenesis. to rapidly attain human novel gene full - length cdna sequence, the gene - specific primers and the vector - specific primers have been designed for successful performing nested pcr and draft human genome searching to rapidly identify the tsarg2 ( genebank accession number ay040204 ) 5 " end from a human testis cdna library by using a cdna fragment ( genebank accession number be644542 ) as a electronic probe, which was significantly changed in cryptorchidism and represents a novel gene. furthermore, a mouse homologue of this gene was identified ( genebank accession number af395083 ) by lab on - line
本研究分為三個部分，其主要實驗方法及實驗結果如下：第一章tsarg2基因的克隆與序列分析從已獲得的在隱睪和正常睪丸對照中表達量有明顯差異的est片段（ be644542 ）入手，設計了基因特異性引物和載體特異性引物進行巢式pcr擴增，結合人類基因組草圖搜索法，從睪丸cdna文庫中快速分離出人類睪丸凋亡相關基因的5末端而獲得全長cdna ， genbank登錄號為ay040204 ，同時應用生物信息學的方法克隆了該基因在小鼠中的同源基因， genbank登錄號為af395083 。
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