guanidinium thiocyanate 中文意思是什麼
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Our efforts were taken to lay the foundation of further studies on cloning and function of new genes in fish. the construction and evalution of the a, gtlo cdna library of grass carp leukocytes are described. total rna was extracted from head - kidney leukocytes using single - step method of rna isolation by acid guanidinium thiocyanate - phenol - chloroform extraction
本文第一部分取病毒感染36小時后的草魚頭腎並分離白細胞，用改進的異硫氰酸胍一步法從其中提取總rna ，磁珠法分離純化mrna ，電泳檢測其質量。 -
In this research, total rna was extracted from fetal liver by the modified single - step method of acid guanidinium thiocyanate - phenol - chloroform ( agpc ). based on reported sequence of hsa cdna, specific primers were designed to amplify its encoding region by rt - pcr. at the same time, kozak sequence was added to the translation site, to strengthen initiation of translation
本研究通過改進的異硫氰酸胍-酸性酚-氯仿一步法從人工流產胎兒肝臟中提取總rna ，然後根據報道的序列設計特異性引物，同時在上游引物中引入了kozak序列，經rt - pcr克隆到hsacdna編碼區序列，長度為1758bp 。
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