high-level expression 中文意思是什麼

high-level expression 解釋
高水平的表達
  • high : adj 1 高的〈指物，形容人的身高用 tall〉；高處的；高地的。2 高級的，高等的，高位的，重要的。3 高尚...
  • level : n 1 水平儀，水準儀；水準測量。2 水平線，水平面；水平狀態；平面，平地。3 水平，水準；水位；標準；...
  • expression : n 1 表現，表示，表達。2 詞句；語句，措辭，說法。3 表情，臉色，態度；腔調，聲調。4 【數學】式，符...
  1. Fusion protein expression system can overcome those problem, increased the yield of yield of recombinant protein in e. coli. this remarkable increase in protein yield was thought to be due to protection of the target protein from proteolysis, improved folding, and efficient mrna translation. fusion protein also make the detection and purification easy, is a good strategies for achieving high - level expression of genes in escherichia coli

    小分子量異源蛋自在人腸桿菌的表達受mrna不穩定、翻譯起始效率低、易被蛋自酶降解等因素的干擾，較難獲得高效表達，通過與已高表達的蛋自融合表達可以克服以上問題，可以使大多數蛋白獲得高效表達。
  2. High - level expression of bydv gav coat protein gene in escherichia coli

    株系外殼蛋白基因在大腸桿菌中的高效表達
  3. The extracellular 100pl ( ecl1 ) and ecl2 was linked by disulfide bond to maintenanced the stability of the protein secondary structure. in recent years, we showed that ccr5 as a co - receptor could interact with hiv - 1 infect ion. ccr5 was paid closed attention to since it was cloned in 1996. the aim is to - obtain the sequence of first extracillular domain of 3 chemokine receptor 5 ( ccr5 ) n - terminal gene fragment with high level expression in e. coli and to prepare its specific antibody f ( ab ' ) ;, and its detected method

    Ccr5具有g蛋白偶聯受體家族（ g - proteincoupledreceptors ， gpcrs ）所特有的7個跨膜區（ transmembrinedomains ， tm ） ，呈螺旋， tm的氨基酸有很高的保守性，膜外第一襻（ extracellularloop1 ， ecl1 ）和ecl2之間有二硫鍵相連，以維持蛋白質二級結構的穩定性。
  4. 4. we use half quantification and digoxigenin detection to investigate the expression pattern of ent - kaurene oxidase. we found that it was regulated by development, it expressed high in the fast growing tissue, expanded leaves under a fast growing circumstance also express high level of ent - kaurene oxidase

    利用半定量rt - pcr的方法結合地高辛標記法，貝殼杉烯氧化酶在果樹中的表達是受發育調控的，其在旺盛生長的組織中表達量高，在植株旺長期的成年葉中表達量也很高。
  5. The constructed plasmid containing gst - src fused gene gave a high level of expression of the fused protein

    用iptg誘導表達蛋白的產生，純化后獲得大量的目的蛋白。
  6. The choice of an expression system for the high - level production of recombinant proteins depends on many factors

    建立hpk - 5因子分離純化工藝； 4建立體外檢測復性蛋白質活性的方法。
  7. Objective : construct high - level expression system of echistatin in e. coli methods : obtain amino - acid sequence of echistatin from genebank database. considering the bias of usage of 61 available aminoacid codons in e. coli, design the coding sequence of echistatin, synthesize the dna sequence chemically, get single copy coding gene and repeated two copy coding gene of echistatin. insert the sequence into expression vector pbv220, and more, we construct fusion expression clone of echistatin with pcr, identify the recombinant vector by dna sequencing

    目的構建蛇毒鋸鱗蝰素（ echistatin ）的原核高效表達體系方法由genebank數據庫檢索蛇毒鋸鱗蝰素（ echistatin ）的氨基酸序列，結合大腸桿菌蛋白質合成體系對氨基酸密碼子使用的偏愛性，設計了echistatin編碼基因，體外人工合成編碼基因dna片段，通過適當的限制性內切酶位點插入表達載體pbv220 ，分別構建了echistatin的單拷貝表達克隆、雙拷貝串聯表達克隆；進一步通過pcr技術構建echistatin的融合表達基因克隆。
  8. The ade + transformants were selected and fermented in flasks with 20ml bmmy medium, then, induced by 0. 5 % methanol. the expression protein was analyzed by sds - page after five days of induction. sds - page analysis revealed that the high - level expression recombinant strains of pmad16 / pmet a b / abp2304 and pmad16 / pmet a a / abp780 had specific bands at 75kd and 55kd separately, account for 30 % and 10 % of the total protein separately, which were purified using probond resin purification system, and obtained 15mg at levels above 0. 75g / l and 7mg expression protein at levels above 0. 35g / l separately once purification, the purity is both above 90 %

    篩選ade +表型轉化子， 20mlbmmy搖瓶培養，用0 . 5甲醇誘導表達5天後， sds - page檢測結果表明：選出的重組高效表達菌株pmad16 pmet b abp2304和pmad16 pmet a abp780都存在明顯的表達特異條帶，分子量分別為75kd和55kd ，分別占其總蛋白的30和10 ，經過probondresin鎳親和層析柱都得到了純化，其純度都在90以上，一次純化分別可得到大約15mg和7mg表達蛋白，推知表達量分別高達0 . 75g l和0 . 35g l以上。
  9. After getting the cdna sequence of curcin, four fragments of the gene were cloned through pcr and high - level expression in e. coli was achieved. the four target dna fragments, i. e

    在得到麻瘋樹毒蛋白cdna序列后，通過pcr對毒蛋白基因進行了克隆，並實現了部分基因片段在大腸桿菌中的高效表達。
  10. High level expression of pngase f in escherichia coli and its bioactivities

    在大腸桿菌中的高效表達及其脫糖基化作用研究
  11. Conclusion constructed the high - level expression clone of echistatin in e. coli. the expression of recombinant protein is higher, it make the further study of echistatin feasible

    結論成功構建了echistatin的原核高效表達克隆，表達量高於現有國內外研究水平，為echistatin功能及相關疾病的研究奠定了基礎。
  12. In this research two full - length cdnas have been cloned by a combination of rt - pcr and 3 " - is1 - race with synthesized degenerate primers from young leaves of vicia faba l., pichia methanolica high - level expression systems of the genes have been constructed, and the milligram expressed protein was purified using probond resin purification system, which may result in further identification of the function of the aba binding protein. the full - length cdna of abp370 fragment is 3449 bp long and has an open reading frame of 2304 bp encoding 768 amino acids with 876 bp long 5 ' - utr, 369 bp long 3 ' - utr and poly ( a ) tail. the full - length cdna of abp640 fragment is 1012 bp long and has an open reading frame of 780 bp encoding 260 amino acids with 88 bp long 5 ' - utr, 144 bp long 3 " - utr and poly ( a ) tail

    3 - 5 - race擴增片段序列分析結果表明， abp370擴增片段的全長cdna為3449核苷酸，其中5非翻譯區為876個核苷酸， 3非翻譯區為369個核苷酸並末端帶poly （ a ）尾巴，從起始密碼子atg至終止密碼子tga ，含有一個編碼768個氨基酸殘基的開放閱讀框架（ 2304bp ） ； abp640擴增片段的全長cdna為1012核苷酸，其中5非翻譯區為88個核苷酸， 3非翻譯區為144個核苷酸並末端帶poly （ a ）尾巴，從起始密碼子atg至終止密碼子taa ，含有一個編碼260個氨基酸殘基的開放閱讀框架（ 780bp ） 。
  13. High level expression of human recombinant albumin gene in the yeast pichia pastoris

    人血管內皮生長因子受體2胞外段在畢赤氏巴斯德酵母中的表達
  14. Construction of high - level expression strain of human acidic fibroblast growth factor

    人酸性成纖維細胞生長因子的高效表達
  15. Refolding and high - level expression study on human interferon - in e. coli

    在大腸桿菌中高效表達和色譜復性研究
  16. These include the ability to target the gene of interest to site - specific areas of the chloroplast genome, high - level expression of foreign genes and increased environment security

    由於植物葉綠體表達系統能夠高效表達目的基因、環境安全性高，已成為目前植物生物反應器研究的熱點之一。
  17. High - level expression and purification of recombinant staphylokinase from engineering bacterium

    重組葡激酶的高效表達與純化
  18. High level expression of human tnf - related apoptosis - inducing ligand extra cellular domain in e. coli

    探討腫瘤壞死因子相關細胞凋亡誘導配體
  19. Chemical synthesis of human interleukin - 18 gene and its high - level expression in e. coli

    人白細胞介素18基因化學合成及其在大腸桿菌中高表達
  20. Strategies to achieve high - level expression of foreign genes in plants are mainly introduced in this paper

    摘要介紹提高外源基因在植物體內表達的方法。
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