homozygous gene 中文意思是什麼
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In 138 han samples, homozygous and heterozygous genotypes of codon 54 allele in mbl structural gene were 2. 17 % and 21. 01 % respectively, while in 120 uigurs samples, those were 0. 83 % and 18. 34 %
17 ，雜合子29例，佔zi刀1 ； 120份維吾爾族標本中，檢出突變純合子1例，佔0 -
Furthermore, the wing imaginal disc clonal analysis showed that in the homozygous sll mutant clones, the extracellular wg was dramatically reduced. the reduction of extracellular wg indicated that the wg signaling in the wing imaginal disc was disrupted. the reason leaded to this phenomenon is that sll encodes a paps transporter, so the disruption of the sll gene would generate the unsulfated heparan sulfate proteoglycan ( hspg ), which most likely would lose normal function
進一步結合運用flp - frt系統和minute ~ - ，用缺損myc蛋白的表達來標記純合的sll突變基因克隆，用minute ~ -來相對的擴大含sll突變基因的克隆，對翅成蟲盤所作的克隆分析表明，在含sll突變基因的克隆中，細胞外的wg蛋白分佈明顯減少，這種細胞外的wg蛋白減少表明在翅成蟲盤中wg信號傳導受到了明顯抑制。 -
The resulting plasmid, named prok - sod2, was mobilized to agrobacterium tumefaciens strain gv3101 used for plant transformation. the yeast sod2 gene was introduced into arabidopsis thaliana ( ecotype landsberg erecta ) by agrobaterium tumefaciens - mediated transformation with floral - dipping method under the control of camv 35s promoter. transformants were selected for their ability to grow on medium containing kanamycin ( 30mg / l ), several homozygous lines that were all tolerant to kanamycin were selected and used for further molecular and physiological determination
本實驗將sod2基因構建到植物表達載體prok中，導入農桿菌后，進行植物遺傳轉化，實現其在擬南芥中過量表達，在含30mg l的卡那黴素的培養基上篩選獲得純合轉基因株系，自交一代獲得足夠的純和轉基因種子后，對其進行了分子生物學的驗證及生理指標的檢驗。 -
Results showed that : ( 1 ) in general, the segregation ratios of target gene in some tl lines are conformed to 3 : 1, however, some are not possibly due to gene silence or missing in the self - pollinated progeny ; ( 2 ) two homozygous plants were identified from 10 putative transgenic plants
主要結果如下： （ 1 ）我們對外源基因在番茄體內的遺傳規律研究表明，在大多數情況下，單拷貝插入外源基因將導致轉基因後代按孟德爾單顯性基因3 1規律分離，但是部分轉化系比例偏低，可能發生了基因丟失或者沉默。
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