host strain 中文意思是什麼

host strain 解釋
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  • host : n 〈常 H 〉【宗教】聖餅，聖餐用麵包。n 1 主人 （opp guest）。2 （廣播、電視的）節目主持人。3 旅館...
  • strain : vt 1 用力拉，拉緊，抽緊，扯緊。2 使緊張；盡量使用（肌肉等）。3 強迫，強制；濫用，盡量利用。4 拉傷...
  1. Since the important roles of eo protein in the viral infection. immunity and virus - host interaction. the homology of 21 csfv strains was investigated by sequence analysis of eo genes in this study, which will provide some evidence for epidemiological study. in addition, the eo gene of hog cholera lapinized vaccine ( hclv ) strain was expressed in the prokaryotic and eucaryotic systems, and the recombinant proteins were preliminarily analyzed by immunological method

    鑒于eo蛋白在病毒感染，誘導機體免疫及與宿主細胞相互關系中的作用，本研究克隆了2株豬瘟病毒eo基因並將其與其它毒株eo基因進行了序列分析，揭示了我國豬瘟病毒流行株之間的遺傳演化關系，為豬瘟病毒的流行病學研究提供依據。
  2. 4. engineering dhqase ( arod ) - deficient e. coli mutant with a second copy of the arob gene gene targeting technique was used to disrupt the arod gene in e. coli chromosome. the mutant 31bk was engineered, in which homologous recombination of the arobkanr gene cassette into the arod locus ( arod : : arobkanr ) of the e. coli strain atcc31884 genome utilized the helper plasmid pkd46 with red system. the host cell 31bk lacked catalytic activity of dhqase ( arod ) and had a second copy of the arob gene, so it improved carbon flow into the quinic acid biosynthesis direction

    構建宿主菌基因精確定位突變株31bk （ arod : : arobkan ~ r ）為了改變代謝途徑脫氫奎尼酸（ dhq ）分支點上的代謝流量，使之充分流向目的產物奎尼酸合成方向，利用基因打靶技術構建了31884宿主菌arod基因精確定位插入突變體，使dhq脫水酶（ dhqase ）失活，阻斷了碳代謝流流向芳香氨基酸生成的方向，同時用同源重組的方法將arob基因定位整合入染色體上，解除了限速酶對碳代謝流通過共同途徑到達dhq的阻遏影響，並減輕代謝負擔。
  3. The advance of the metabolic engineering was briefly reviewed and the application of metabolic engineering methods for the construction of d - ribose - producing genetic engineering strain such as host selection, rate - limited step acceleration, metabolic flow alteration, fermentation process optimization and its application prospects of metabolic engineering was also described

    摘要簡要介紹了代謝工程的進展情況，並較為詳細地從宿主的選擇、加速限速反應、改變代謝流和生產過程的優化等方面論述了代謝工程在d -核糖基因工程菌構建方面的應用及其應用前景。
  4. This paper concerns studies on rhizobia in symbiosis with important legume soybean and peanut crops in china, following : 1 ) summarized bio - diversity of rhizobia, including indigeneous population, isolation frequency, strain type, distribution, and strain - host promiscuity and compatibility from large and small samples. 2 ) reviewed symbiont improvement in both sides of rhizobia and hosts, involving selection of effective rhizobial strains, evoluation and utilization of the host germplasm for symbiotic properties, and selection of the both sides for special characters. 3 ) discussed the subject on the rich symbiont germplasm resource in china and on further research being necessary to exploitation and utilization of the benificial germplasm

    針對我國主要豆科作物大豆和花生的研究成果，並結合本身研究工作內容， （ 1 ）總結了與宿主共生的根瘤菌生物學多樣性，包括大、小樣本的土著根瘤菌群體數量、分離頻率、類型與分佈及其菌株宿主共生混交性與親和性； （ 2 ）評述了根瘤菌宿主植物共生體雙邊固氮改良，包括優良菌株的選育、宿主品種資源共生特性的評價與利用及特異性狀的選擇； （ 3 ）討論了我國這一類共生體資源的豐富性以及有必要進一步加大力度研究有益資源的開發和利用的問題。
  5. Abstract : this paper concerns studies on rhizobia in symbiosis with important legume soybean and peanut crops in china, following : 1 ) summarized bio - diversity of rhizobia, including indigeneous population, isolation frequency, strain type, distribution, and strain - host promiscuity and compatibility from large and small samples. 2 ) reviewed symbiont improvement in both sides of rhizobia and hosts, involving selection of effective rhizobial strains, evoluation and utilization of the host germplasm for symbiotic properties, and selection of the both sides for special characters. 3 ) discussed the subject on the rich symbiont germplasm resource in china and on further research being necessary to exploitation and utilization of the benificial germplasm

    文摘:針對我國主要豆科作物大豆和花生的研究成果，並結合本身研究工作內容， （ 1 ）總結了與宿主共生的根瘤菌生物學多樣性，包括大、小樣本的土著根瘤菌群體數量、分離頻率、類型與分佈及其菌株宿主共生混交性與親和性； （ 2 ）評述了根瘤菌宿主植物共生體雙邊固氮改良，包括優良菌株的選育、宿主品種資源共生特性的評價與利用及特異性狀的選擇； （ 3 ）討論了我國這一類共生體資源的豐富性以及有必要進一步加大力度研究有益資源的開發和利用的問題。
  6. Beijing ' s effort to host an olympic games untainted by criticism of its foreign and domestic policies has come under unprecedented strain amid growing international outrage over chinese support for the government of sudan

    中國政府希望在不受對其內政及外交政策批評的影響下主辦奧運會，但在國際社會日益對中國支持蘇丹政府表示憤慨的情況下，中國政府的努力遇到了前所未有的壓力。
  7. It was then cloned to the secreted vector - ppic9k and recombined successfully into the chromosome of pichia pastoris host strain - gsl 15 by electroporation

    通過電轉化作用該基因片段被成功地整合至酵母cs115的染色體上，經過甲醇誘導之後，該基因得到了分泌表達。
  8. Transformation was done by electroporation. human fl extracellular domain cdna transformed to yeast host strain km71, then his + muts phenotype transformant was screened out and cultured in flasks, and rhfl was expressed under the induction of 0. 5 % methanol

    我們提取了km71ppic9k - fl轉化菌株的基因組dna進行southern實驗，檢測目的基因的整和插入；提取總rna ，進行了northern實驗，檢測fl基因在轉化菌株中的表達。
  9. A gene library of psedomonas fluorescens g2 was constructed in the cosmid vector pla2917 using e. coli jm109 as the host strain. two recombinants, pgr3 and pgr7, which can confer glyphosate resistance ofe. coli jm109 were identified from the selective medium containing 10mm glyphosate

    以粘粒pla2917為載體、大腸桿菌jm109為受體菌構建熒光假單胞菌g2的基因組文庫，在含有10mm草甘膦的固體選擇培養基上篩選出兩個耐受克隆pgr3和pgr7 ，插入片段分別為7kb和11kb 。
  10. The recombination vectors were transformed into host strain of bl21 and induced with iptg. all the recombinant protein was expressed into inclusion body. the recombinant protein was identified with sds - page and westen - blot and purified through elution method. the muticlone antibody was got by immuning the rabbit with the purified protein

    把重組質粒轉化入表達受體菌bl21 ，經iptg誘導后都獲得了表達，且都以包涵體的表達形式存在，用sds - page和western - blot的方法對重組蛋白進行了鑒定。
  11. The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins

    將帶有hwtx -基因的ppic9k經blgii線性化后，轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發酵上清經90飽和度的（ nh _ 4 ） _ 2so _ 4沉澱， yw - 3 （ mwc03000 ）的超濾膜超濾，再經cm陽離子交換， c _ （ 18 ）反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經質譜鑒定，氨基酸組成分析和序列測定為正確的表達產物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。
  12. The fragments were ligated directly to the pichia pastrois expression vector ppic9 to got ppic9 - e3and ppic9 - e8. vectors were amplificated in the e. coli dh5 a and were linearized with bgl ii. the linearized vctors were transformed into host strain gs115. the recombinated strain was selected though phynotype and pcrthe positive strain was induced with methyl alcohol and was selected by dot - elisa. the recombinated protein was detected with sds - page and western - blot as before

    重組菌用甲醇誘導表達，用dot - elisa的方法篩選到表達量較高的菌株。將篩選出的菌株大量的誘導表達，對表達上清處理后，用sds - page和western - blot進行鑒定。同時，用hiprep16 10heparinff肝素親和柱對表達蛋白進行了初步的純化。
  13. All the result showed that ndv f48e9 strain has its own speciality compared with other five ndv strains, and there were many difference between velogenic strains and lentogenic strains. so the infectious cdna of rnesogenic strains and lentogenic strain was far from enough to understand the replication, pathogenicity of ndv and the interaction between ndv and host cells, and the infectious cdna of velogenic strains ( eg. f48e9 ) was required to explain the relationships between structure and function

    本研究成功地獲得了ndvf48e9 t因組的核昔酸序列，並構建了表達ndvf48e9基因組cdna的低拷貝表達載體休f48e9 ，為構建新城疫病毒強毒株f48e9株的感染性cdna奠定了物質基礎，進一步研究ndv的生物學特性、結構與功能的關系；進一步探討影響ndv毒力的因素、以及研製新型疫苗載體提供了可靠保證。
  14. According to their pathogenic activity, three strain of marine bacteria were selected as l2 ( alteromonas sp ), g ( pseudomonas sp ), py ( pseudomonas sp ). the biochemical changes of malondialdehyde ( mda ), which is the lipid peroxidation end product and can be measured by thiobarbituric acid ( tba ) regeant, and polyphenol content, which reflected the host non - specific chemical defense activity and can be measured by folin - ciocalteu method, were determined at different time intervals during host pathogenesis

    本研究採用海帶作為模式藻類生物，以褐藻酸降解菌l2 ， g及非褐藻酸降解菌py為復染菌建立人工復染體系，用硫代巴比妥酸（ tba ）試劑測定抗感染過程中海帶組織細胞膜脂肪氧化產物丙二醛（ mda ）的變化情況及folin - ciocalteu酚試劑測定病原菌感染過程中海帶組織中多酚含量隨時間變化情況。
  15. Kurstaki strain hd73, were inserted into two copy sets of res sites. the res sites have same direction. when - the recombinant plasmid was introduced into crystal negative b. thuringiensis host bmb171, antibiotic resistance genes and other non - 5, thuringiensis dna can be selectively eliminated after the selection by antibiotic resistance marker

    將crylac10基因或壯觀黴素基因和蘇雲金芽胞桿菌的質粒復制起始區oril030連接在一起，置於兩個同向的解離區之間，再將基因操作中所必需的大腸桿菌質粒復制起始區和抗生素標記基因等與之相連構成解離載體。
  16. The most variable regions between hi and b strain reside in vp2 and vp3 overlap region ( 319 - 470 ), which are supposedly exposed to the surface of the capsid. it is possible due to the selective pressure from host immune system. the largest divergence between the gpv - h1 and mdpv capsid polypeptides located between the start cordons of vp2 and vp3

    H1株與gpv - b株氨基酸的差異集中在vp2 、 vp3重疊區的319 - 470位之間，這與預期暴露于病毒最表面的區域相吻合，可能是宿主免疫篩選導致的變異； gpv - h1株和mdpv之間還存在另一高變區，位於vp2和vp3起始密碼子之間，這可能與兩病毒有不同宿主域相關。
  17. Attachment to host tissue is essential for colonization by most mucosal pathogens, such as mycoplasma hyopneumoniae. according to the reported sequence of p97 gene in strain 168, a pair of primers was designed and the rl region of p97 gene in strain 168f485 was amplicated by pcr

    本研究根據已報道的序列為參考，設計一對帶有ecor和hind酶切位點的引物，並通過引物的定點突變，利用pcr方法從豬肺炎支原體168f485株擴增到其黏附因子p97基因的抗原決定簇r1區。
  18. It has made the strong basis for further studying mechanism of replication, virulence and determinant, attenuation, pathogenesis, functions of genetic products, specific diagnosis, cell and host tropism, development of dna vaccine and marker vaccine of csfv, and provided an excellent tool for molecular virology. main research contents include : based on published nucleotide sequences of csfv and by the help of computer analysis software, high conservative regions and single restriction enzyme sites of genome were selected. utilizing rt - pcr and nested - pcr techniques, 7 overlapping cdna fragments covering the full genome of csfv c - strain were successfully amplified

    中國豬瘟兔化毒（脾淋毒）基因組cdna文庫的構建、序列分析：根據已發表的豬瘟病毒（ csfv ）核苷酸序列，藉助計算機軟體分析，選擇高保守區段和基因組中的單一限制性酶切位點，利用rt - pcr及nested - pcr和helf - nestedpcr技術，成功地擴增出了覆蓋c -株全基因組的7個cdna重疊片段f1 f7 ，分別克隆到pmd - 18t或pgem - teasy載體進行測序后，拼接出了其核苷酸序列。
  19. A review on maize dwarf mosaic disease is presented, including its symptom, distribution, damage, host range, viral strain, viral transmission and control method. future directions for the research of maize dwarf mosaic disease are also recommended

    介紹了玉米矮花葉病的癥狀、分佈、危害、寄主范圍、病毒株系、病毒傳播及防治方法等方面的研究進展。
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