hygromycin 中文意思是什麼

hygromycin 解釋
潮黴素
  1. A genetic transformation model for the brown seaweed undaria pinnatifida has been primarily set up by using micro - particle bombardment as the method, female or male gametophytes as the gene recipients, hybridization as the regeneration route and chloramphenicol, hygromycin or basta as selective reagents

    本文從轉化受體、轉化方法、報告基因、再生途徑、篩選方法等方面對裙帶菜的遺傳轉化進行了研究。首先,分離並建立了裙帶菜雌雄配子體的無性繁殖系,進行了裙帶菜的不同再生途徑的研究。
  2. By using primers designated for lacz gene, pcr result suggested an integration of lacz into u. pinnatifida genome. nine reagents including penicillin g, kanamycin, g418, teicoplanin, zeocin, chloramphinicol, hygromycin, basta and methotrexate were tested as selective reagents for selection of transgenic sporophytes. the results showed that young sporophytes of u. pinnatifida were sensitive to chloramphinicol and hygromycin and very sensitive to basta which suggest the potential of using the resistance genes as selectable markers

    ,為了解決轉基因裙帶菜的篩選問題,進行了裙帶菜幼孢子體對不同篩選壓力的敏感性實驗,包括抗生素類的青霉素g 、卡那黴素、 g418 、硫酸鏈黴素、鹽酸潔黴素、 zeocin 、氯黴素、潮黴素,除草劑類的basta ,氨甲喋呤類的氨甲喋呤,結果顯示裙帶菜幼孢子體對氯黴素、潮黴素敏感,對basta非常敏感。
  3. The results also revealed that it was a reasonable choice to use carbenicillin as the antibiotics of inhibiting growth of remnant agrobacterium after co - culture, and when hygromycin was used as the selective agent, continuous selection at low concentrations ( 50 ~ 150mg / l ) produced the highest numbers of transgenic plants without escapes

    試驗表明,在農桿菌介導高羊茅遺傳轉化中,抑菌劑宜選用梭節青霉素;以潮黴素作為選擇劑時,採用低濃度( 50一15om留l )連續篩選的方式比較合適,在該方式下,獲得的轉基因植株較多。
  4. These vectors were tried to transform into the p. digitatum imazalil - sensitive isolate by the methods of peg - mediated or electroporation transformation. after that, the transformed protoplasts were regenerated on czapek medium containing hygromycin at 300 # g / ml. unfortunately, the ideal transformants could not be obtained

    採用peg法和電擊法兩種方法進行指狀青黴原生質體的轉化,然後將得到的轉化產物轉移到查氏再生培養基中培養,遺憾的是沒有得到理想的轉化子。
  5. We obtained our transgenic material, the rice suspension cells, by inducing embryonic rice callus. then we constructed the expression vector pca - ced9, and transferred ced - 9 gene into the rice callus and embryogenic suspension cells. the work of using hygromycin selective medium to obtain regenerated plants is still going

    構建了用於水稻中表達的載體pca - ced9 ,通過農桿菌eha105轉化導入水稻愈傷組織和水稻懸浮細胞,經潮黴素( hym )篩選,以期望獲得抗性植株,目前該工作仍在進行中。
  6. Next, the researchers exposed the mushroom cells to hygromycin. the antibiotic kills all the normal cells, separating out those that hae been genetically altered for resistance

    接著,研究人員用這些蘑菇細胞去接觸潮黴素。這些抗生素可以殺死所有正常的細胞,分離出哪些能抵抗潮黴素的細胞。
  7. The predicted bands were amplified from both to and ti generation transgenic plants by pcr, indicating that the hygromycin resistance gene ( hpt ) and svde gene were both transferred into tobacco

    Pcr擴增潮黴素抗性基因hpt和svde基因結果顯示,在『轉淬回植株t斤t ;代中都分別擴增出互okb和二
  8. To create transgenic mushrooms, researchers attached a gene that confers resistance to hygromycin, an antibiotic, to circular pieces of bacterial dna called plasmids, which hae the ability to multiply within a bacterium known as agrobacterium

    為了創造轉基因蘑菇,研究人員需要把一種抵抗潮黴素(一種抗生素)的基因片段插入細菌dna的圓形片段(也叫質粒)中去,它將隨著土壤桿菌復制而大量復制。
  9. When the seeds from to transgenic plants were cultured on medium supplemented with 50mg / l hygromycin, the ratio of germinated to non - germinated seeds is 3 : 1, which is consisted with mendel ' s first law. southern blotting demonstrated that the svde gene had been integrated into the genome of tobacco plants

    轉基因植株的l代種了在潮黴素培養基上的萌發數與未萌發數的比值為3 : l ,符合單基因的盂德爾分離規律。
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