in-vitro embryos 中文意思是什麼
in-vitro embryos
解釋
試管胚胎-
In vitro culture of mature embryos of ginkgo biloba l
銀杏成熟胚的離體培養研究 -
Background : in - vitro maturation ( ivm ) of human oocytes would be an attractive alternative to gonadotrophin stimulation for in - vitro fertilization ( ivf ). it could avoid the complications of gonadotrophin stimulation and reduce the cost of drugs. however, the efficiency of existing ivm technique is suboptimal because embryos resulting from ivm oocytes exhibit frequent cleavage blocks and low implantation rate
研究背景：近20年來，輔助生育技術有了突飛猛進的發展，體外受精、卵漿內單精子注射技術（ intracytoplasmicsperminjection ， icsl ）和種植前遺傳學診斷技術的成熟標志著人類生殖醫學進入了嶄新的領域，但同時也不能否認，在這一領域中還存在諸如妊娠率低、藥物副作用和多胎率高等問題。 -
Abstract : the early embryo developmental block is a common phenomenon in mammal when embryos are cultured in vitro. many studies of phosphorus, glucose, hypoxanthine and cytoplasmic factors on early embryo developmental block carried out by different methods such as morphology, biochemistry, molecular biology and micromanipulation have been reviewed. the merit and shortcoming were analyzed and the necessity of using simple or components limited media overcoming early embryo developmental block were also reviewed. media that have been shown effective in overcoming early embryo developmental block in mouse, rat, hamster, rabbit, pig, sheep, cattle and monkey were listed
摘要哺乳動物胚胎在體外培養中普遍存在早期發育阻滯的現象.對此，人們用形態學、生物化學、分子生物學、顯微操作等手段開展了磷酸、葡萄糖、次黃嘌呤和細胞質因素對早期胚胎發育阻滯的影響的研究.本文綜合分析了共培養系統的優缺點.說明了採用完全成分已知的培養液對進行有關研究的必要性.列出了有效運用於克服小鼠、大鼠、倉鼠、兔、豬、羊、牛、猴等動物早期胚胎阻滯的成分已知的培養液的名稱。 -
The mice showed also ageing - associated decline in oocyte quality, as indicated by increased frequency of premature chromatid separation, spindle disruption and / or chromosome misalignments in mii oocytes of aged mice. the gv oocytes from aged mice normally matured in vitro. the percentages of pronuclear - stage embryos, 2 - cell embryos and blastocyst derived from mii oocytes of aged mice by in vitro fertilization ( ivf ) were comparable with ones of young mice, but pronuclei formed 1 h later in aged mice than in young mice
老齡小鼠gv期卵母細胞能正常體外成熟；老齡小鼠m期卵母細胞經體外受精后形成原核期胚、 2 -細胞期胚和囊胚的比率與年輕小鼠比較無顯著差異，但原核形成時間延遲1小時；在老齡小鼠卵母細胞，注射牛精子抽提物或sr ~ （ 2 + ）處理時所誘發的ca ~ （ 2 + ）振蕩以及在減數分裂成熟及早期胚胎中的pkc分佈未發生改變。 -
Objective the present study was to investigate the effects of endotoxin on capacitation and acrosome reaction ( ar ) of mouse, golden hamster and human sperm, on the sperm - oocyte fusion in mouse, and on the development of 1 - cell, 2 - cell and zona - free 2 - cell mouse embryos in vitro. the purpose was to definite the committed step and the mechanism during in vitro fertilization ( ivf ) on which endotoxin affected, and to distinguish the sensitivities to endotoxin of three developmental systems of mouse embryos. all these data would provide the reference to clinical and laboratory quality control
目的研究內毒素對小鼠、金黃地鼠及人精子的體外獲能和頂體反應、小鼠精卵結合及小鼠1 -細胞、 2 -細胞和去卵透明帶2 -細胞胚胎體外發育的影響，探討內毒素影響體外受精結局的環節及可能的機制，確定三個體外培養系統對內毒素的敏感性，以期為臨床和實驗室質量控制提供參考。 -
In order to improve the cuture system of mammal embryo and advance the quality of embryo in vitro, the effects of taurine and ethylenediaminetetraacetic acid ( edta ), estrogen ( e2 ) and progesterone ( p ) on preimpiantation development of mouse embryos in vitro were examined and the quality difference of blastocysts from in vitro and in vivo by blastocyst staining and embryo transfer was evaluated
為完善體外培養體系，提高體外發育的胚胎質量，本實驗研究了牛磺酸（ tau ） 、 edta 、雌激素（ e2 ）和孕激素（ p ）對昆明白小鼠胚胎體外發育的影響。在此基礎上，通過胚胎移植技術觀察了不同來源的囊胚在移入子宮后的發育情況，以比較體外培養和體內發育的囊胚質量。 -
Techniques in embryo technology ( such as in vitro production of embryos and animal cloning ) need large quantities of high quality oocytes. but the quality of in vitro matured oocytes from slaughtered animals is generally lower than that of the in vivo matured oocytes. it is usually thought that the reason for this poor quality in in vitro matured oocytes is the lack of capacitation during the dominancy of follicular development in vivo
目前胚胎工程技術研究和開發（如體外生產胚胎和體細胞克隆等）需要大量高質量的成熟卵母細胞，但利用屠宰動物卵巢卵母細胞經過體外成熟培養而獲取的卵母細胞質量還遠不如體內成熟卵母細胞，其原因一般認為是由於缺乏體內主卵泡階段的獲能作用。 -
The technique of microinjection is significant to embryo ' s development. for example, the different volume of microinjection will have direct effect on the in vitro survival rate of the embryos and even the successful rate of the transgenic animals
顯微注射技術對胚胎發育有著關鍵性的影響，如注射劑量的不同將直接影響到胚胎體外發育的存活率乃至影響整個轉基因動物的成功率。 -
The stage when the block occurs has been correlated to the time the embryonic genome takes over control of development from the maternal genome, which occurs in mouse at the 2 - cell stage. the embryos of balb / cxicr fl mouse show 2 - cell block in vitro and the effects of several culture media on overcoming the 2 - cell block in embryos development were studied. m16 ( sigma ), in which 96. 2 % of 1 - cell embryos developed to 4 - cell stage, were shown to be effective in overcoming the 2 - cell block
培養5h后， 53去核卵母細胞可見標記的mtocs ，但數量明顯少於對照組，而又多於培養0h的去核細胞，說明此時53的化學去核卵胞質中確有mtocs的存在，其微管聚合能力正在恢復； mtocs標記，但其輻射產生的微管纖維短，結構還不夠豐滿；其餘細胞的微管蛋白則喪失重新裝配能力。 -
Birth of mouse offspring after transferring of embryos in vitro cultured furt her demonstrated the availability of the culture system of mouse embryos
繼續加長培養時間的試驗表明， m1 』 0cs的恢復類似於培養10h ，微管組裝能力己經最大幅度恢復。 -
According to the reviewers, about 80 percent of women who undergo in vitro fertilization will reach the embryo transfer stage with good - quality embryos, but “ only a small proportion will then go on to achieve a clinical pregnancy and fewer will achieve a live birth
根據評價人員的說法，大約80 %接受體外受精的女性會具有發育完好胚胎而進入胚胎移植期，但是「只有小部分可以繼續完成臨床懷孕，並且有較少女性可以達到嬰兒安全出生。 」 -
Our research used this laser system and its precise measurement software to make a all - round study on the relationship of zona pellucida thickness / hardness variation of human embryos and clinical pregnancy outcome following in vitro fertilization treatment and the application of laser thinning in assisted hatching. the purpose of this study was to try to provide the basic rationale for assisted hatching and an alternative assisted hatching method
本研究即為應用這種激光系統及其精確的測量軟體，對zpt 、 zptv與妊娠結局的關系、體外培養胚胎透明帶硬度的變化及激光薄化透明帶進行輔助孵出的臨床應用進行綜合研究探討，旨在為輔助孵出的病人選擇、方法選擇提供基礎與臨床理論依據。 -
The study of human embryos cultured in vitro indicated that the survival rate and viability of embryos cultured in vitro is much lower than those in vivo
對在體外培養的人胚胎的觀察發現，大約有50的人胚在發育至胚泡期之前停止發育。 -
This study did microinjection to kunming white mouse inbred embryos to test its in vitro developmental ability, then pluripotency of porcine eg cells was tested with production of chimera by blastocyst injection. finally, first chimeric pig in china was delivered, and by chimera analysis, chimerism is present in some tissues
本研究首先利用近交系昆明白小鼠進行胚胎顯微注射觀察發育效果，然後利用所獲五指山豬eg細胞製作嵌合體檢驗eg細胞多能性，最終獲得國內首例胚胎幹細胞（ eg細胞）嵌合體豬，經dna分析確認在多種組織中存在嵌合狀態。 -
Effects of ethylenediaminetetraacetic acid, energy substrate, amino acids on in vitro maturation of porcine oocytes and in vitro development of porcine parthenogenetic embryos
氨基酸對豬卵母細胞的體外成熟和早期孤雌發育的影響 -
Some interest has been generated in studying the rationale for assisted hatching. some reports suggest that embryos with lower zona pellucida thickness ( zpt ) and higher zona pellucida thickness variation ( zptv ) had a better chance of successful hatching and so the patient become pregnant ; another reports suggest that in vivo aging ; in vitro culture and exposure to ultralow temperature may induce zona hardening and then impair the embryo ability of hatching
有作者提出胚胎透明帶厚度（ zonapellucidathickness ， zpt ）小的胚胎孵出時阻力減小，而透明帶厚度變化率（ zonapellucidathicknessvariation ， zptv ）高的胚胎由於透明帶上有一個明顯薄的區域而使胚胎易於孵出；還有作者推測病人年齡、體外培養天數的增加及冷凍處理會增加透明帶的硬度，使胚胎孵出困難。 -
Taken together, these results indicated that an understanding of how in vitro oocyte maturation, in vitro fertilization and embryo culture systems influence both preimpiantation embryo and fetal development should result in systems that consistently produce normal embryos and fetuses
由此說明，體外受精的胚胎的質量顯著低於體內受精的；體外培養液中同時添加雌激素和孕激素能協同促進小鼠胚胎的體外發育，提高胚胎移植后的發育潛力。 -
This study were designed to investigate development potency of the reconstructed embryos of black bear - cat inter - species nuclear transfer in vitro / vivo, to develop the principle and technology of somatic nuclear transplantation in inter - species of mammalian and to study the mechanism of coordination between cell nuclear and cytoplasm. somatic nuclear transplantation technology has been used to establish stem cell from different animals, which can provide materials to treat various diseases
本研究運用體細胞核移植的原理和技術，以六月齡雌性黑熊的成纖維細胞為供核體，去核未受精家貓成熟卵母細胞為受體，構建種間核移植重組胚胎，研究種問體細胞核移植胚胎的構建方法和重組胚胎的體內外發育潛能及其影響因素。 -
This study were designed to investigate development potency of the reconstructed embryos of rat - mouse and rat - bovine inter - species nuclear transfer in vitro / vivo, to develop the principle and technology of somatic nuclear transplantation in inter - species of mammalian and to study the mechanism of coordination between cell nuclear and cytoplasm. somatic nuclear transplantation technology has been used to establish stem cell from different animals, which can provide material to treat various diseases
本研究運用體細胞核移植的原理和技術，以成年大鼠的成纖維細胞為供體，去核小鼠和牛成熟卵母細胞為受體，構建種間核移植重組胚胎，研究種間體細胞核移植胚胎的構建方法和重組胚胎的體內外發育潛能及其影響因素。 -
The preimplantation embryos of several mammalian species undergo blocks in development in vitro
當然目前該方法體系還不成熟，有待于進一步研究和探索。
分享友人