indirect elisa 中文意思是什麼

indirect elisa 解釋
間接酶聯免疫吸附測定,間接elisa
  • indirect : adj. 1. 間接的,第二手的;迂迴的;曲折的。2. 不直截了當的,不坦率的,不誠實的。adv. -ly ,-ness n.
  • elisa : 埃莉薩
  1. After the protein refolding of denaturant inclusion body following dialysis, we got the pure recombinant gst - eo protein by gst affinity columns. using the purified protein as coating antigen, an indirect elisa were developed for detecting the anti - eo antibody in the csfv serum by exploring the concentration of coating an tigen and dilution degree of serum

    使用分步透析法對變性的包涵體進行復性,將復性蛋白過gst親和層析柱得到純化的gst - eo融合蛋白。以gst - eo融合蛋白為診斷抗原,初步建立了用間接elisa檢測豬瘟血清eo抗體的方法。
  2. Rules of indirect elisa technique for equine infectious anemia disease

    馬傳染性貧血病間接elisa技術規程
  3. Test 3 : detected activity of serum and immunoglobulin samples by indirect elisa test, rabbit antibody against foxes ' igg and hen ' s labeled with hrp igy was used in indirect elisa test

    試驗三:間接elisa檢測的抗體活性。用過氧化物酶標記的兔抗狐igg和雞igy抗體進行間接elisa測定獲得的樣品的活性。
  4. The average csfv titer of samples is 1 : 32 by indirect elisa, but 1 : 4 dilution is used in practice

    通過方陣試驗找出了mcab和血清多抗( pcab )的最佳工作稀釋度。
  5. 2 - e4 - a and 82 - 6 are hybridized during their log growing time, and the hybrid - hybridomas are cloned for 3 times and produce 6 hybrid - hybridoma cells. the chromatosome of hybrid - hybridoma 3 - hu and hybridoma 2 - e4 - a and s2 - b are counted, and the antibody of ascites fluid or culture supernatant of 3 - hn is prepared. the positive clones are detected by three methods at the same time : rbc agglutination for monospecific anti - human rbc type a antibody, indirect elisa for anti - p24 antibody, and rbc solid - phase adherence for bispecific antibody

    選其中一株3 - h _ ( 11 )做雜交-雜交瘤細胞染色體計數,同時計數兩母株2 - e _ 4 - a和s _ 2 - b的染色體數:制備腹水型和上清型抗體,用三種方法同時檢測其中的雙特異性抗體、單特異性抗人紅細胞抗體和抗p24抗體,即紅細胞固相吸附法測雙特異中文摘要性抗體,紅細胞凝集試驗測單特異性抗人a型紅細胞抗體,間接elisa法測抗p24抗體;用腹水型抗體做耐熱性及耐凍融實驗。
  6. It was testified that the antibody can special immunological recognition the protein gst - cpti and cptl by indirect enzyme linked immunosorbent assay ( elisa ). the coefficient of correlation is significant and the potency is more than 1 : 800

    經間接elisa法檢測,抗體能與gst - cpti 、 cpti蛋白特異性結合,其相關系數達到顯著水平,效價1 800 。
  7. From dead chicken which infected infectious stunting syndrom of our province, one virue was isolated using spfeggs, chicken embryo fibroblast, mdck18, and vero cell. this virus was unable to agglutinate chicken erythrocytes. in order to definite the pathogeny of infectious stunting syndrom. physical and chemical specific property, types of the nucleic acid of the isolated virus, recurrent infection and other biological property determination and indirect elisa test proved it as a parvoviruses like strain of chicken

    為確定該病的病原,對所分離病毒進行了理化特性測定、病毒核酸型別測定、動物回歸試驗等生物學特性測定,證明該分離病毒與細小病毒科( parvovirdae )細小病毒屬( parvovirus )的雞細小病毒( chickenparvovirus )特性基本相符,核酸型為dna型。
  8. Colibacollosis serotype k. 99 positive sera in ducks and duck plague positive sera were tested, whereas, the results of detection to r. a. sera were positive. the sensitivity of indirect elisa were 50 to 100 times ( serotype 1 ), ( 2 ) 25 to 100 times ( serotype 2 ), ( 3 ) 12 to 100 times ( serotype 4 ) and 25 to 200 times ( serotype 5 ) th an micro - agglutination test

    包被鴨疫里默氏桿菌抗原時,對鴨抗5 : a多殺性巴氏桿菌陽性血清、鴨抗型鴨病毒性肝炎陽性血清、鴨抗鴨源大腸桿菌k _ ( 88 )陽性血清、鴨抗鴨源腸炎沙門氏菌陽性血清、鴨抗鴨源大腸桿菌k _ ( 99 )陽性血清、鴨抗鴨瘟陽性血清檢測結果呈陰性。
  9. 3 immunogenicity of m3m4 loop recombinant peptide antigen of nr1, neuroexcitotoxicity protective effects and their mechanisms study of antiserum against m3m4 ( 1 ) to explore the immunogenicity of m3m4 loop recombinant peptide vaccine, m3m4 recombinant peptide was injected subcutaneously to immunize balb / c mice ( h - 2 ), cd4 / cd8 t lymphocytes subsets, the cytokine levels in serum and the liter of specific humoral response were detected with facs, indirect elisa and elisa separately

    ,抗血清還可以結合合成膚nps ,證明該表位具有免疫原性和抗原性。收集抗血清用於功能檢測。 (二) m3m4片段抗血清抗興奮毒性損害作用以臺盼藍染色法和原位末端標記( tun 』 el )法觀察抗血清對原代培養海馬神經元興奮毒性壞死和凋亡的保護效應。
  10. Vaccine, and to monitor the antibodies level of unvaccinated ducks. the response curves were established when titers were varies along with the time, and the results showed that the indirect elisa could used to predict the efficiency of vaccination

    應用建立的間接elisa方法,對40隻用油佐劑滅活疫苗免疫后鴨子的抗體消長進行了測定,結果表明該方法適于作為疫苗免疫效果的評價。
  11. 107 + 2. 819x ( serotype l ), log [ et reciprocal ] = 1. 019 + 2. 935x ( serotype 2 ), ( 3 ) log [ et reciprocal ] = 0. 99 + 2. 709x ( serotype 4 ) and log [ et reciprocal ] = 1. 052 + 2. 953x ( serotype 5 ). the derived regression lines ( equations ) were used to transfer the optical density value ( od ) obtained from a single 1 : 100 dilution of any unknown serum sample of duck into elisa titer. the indirect elisa technique were used to monitor the variety of the mean antibodies titers of vaccinated ducks with the oil - emusified formalin - inactived r. a

    間接elisa方法的靈敏度是微量凝集試驗的50 - 100倍(血清1型鴨疫里默氏桿菌) , 25 - 100倍(血清2型鴨疫里默氏桿菌) , 12 - 100倍(血清4型鴨疫里默氏桿菌) , 25 - 200倍(血清5型鴨疫里默氏桿菌) 。
  12. Methods : the balb / c mouse is immunized with gene recombinant antigen p24 for four times in 2 months. the spleen cells of immunized mouse is hybridized with sp2 / 0 by peg, and the positive cell clones secreting the antibody to antigen p24 are detected by indirect elisa. through three clonings less diversed anti - p24 hybridoma cells are gained

    方法:基因工程p24抗原免疫小鼠4次,歷時2個月,取脾細胞與骨髓瘤細胞株sp2 0 ,用peg融合, hat選擇培養和間接elisa篩選分泌抗p24抗體陽性的雜交瘤細胞,三次克隆化后得穩定分泌抗p24抗體的雜交瘤細胞株。
  13. The screening of supernatants was carried out with using indirect elisa. after subcloning by limiting dilution and selection. five clones of cells with high od value were acquired and named mvi

    過陽性雜交瘤細胞的篩選和有限稀釋法的克隆化步驟,獲得了5株od值高的單克隆細胞株,編號為mv1 , mv2 , mv3 , mv4 , mv5 。
  14. 2. the putative epitopes that displayed on phages were identified by indirect elisa using swine antisera against prrsv and mouse antisera to recombinant structural protein of prrsv. seven putative epitopes could be recognized by antisera

    利用prrsvbj - 4陽性血清和鼠源抗重組結構蛋白抗體,採用間接elisa方法對噬菌體展示的表位進行了鑒定。
  15. By immune the animal with the protein, the antibody which is anti - gst - cpti fusion protein was prepared. lt was testified that the antibody can special immunological recognition the protein gst - cpti, gstand cpti by indirect elisa. the coefficient of correlation is significant and the potency is more than 1 : 8000

    對其進行細菌表達后,利用glutathionesepharose4b親和柱純化獲得gst - cpti融合蛋白,免疫動物后制備了相應的抗gst - cpti融合蛋白抗體。
  16. And the binding specificity of the scfv fusion protein with hbsag was confirmed by indirect elisa

    間接elisa檢測證實所表達的單鏈抗體具有與hbsag結合的特異性。
  17. Immuno - pcr for il - 6 was established by use of 1ng / l of the biotinylated dna as template. this immuno - pcr has a detection limit of 1fg / ml of pil - 6 that is 105 times lower than that of indirect elisa, and is the most sensitive method to detect il - 6 up to date

    通過與elisa法比較,發現新建立的免疫pcr法靈敏度為1fg ml豬il - 6 ,是elisa法的10 ~ 5倍,是目前已報道的檢測豬il - 6最靈敏的方法。
  18. The mcabs except for dg5 could react with csfv e2 protein in indirect elisa, indicating that ac9, cf8 and ec9 are anti - csfv e2 protein mcabs

    四株單抗與基因工程csfve2蛋白反應結果表明ac9 、 cf8和ec9是抗csfve2蛋白的單抗。
  19. Two weeks after the second boost, blood was collected and the antisera were tittered using indirect elisa, the liter was beyond 1 : 1600 and accorded with the demands of producing antisera

    完成免疫方案之後,取血,並用另一隻未經免疫的小鼠作陰性對照,測定效價為1 / 1600 ,符合單抗制備的要求。
  20. The activity of the purified product was confirmed by indirect elisa analysis and was further confirmed by indirect immunofluorescence and immunohistochemistry after they were added to the culture medium of hepatocarcinoma cells

    在大腸桿菌blzi中實現了scfv融合蛋白的表達。經ni nta柱純化獲得純化產物,經間接elisa分析確定所得產物具有與hbsag結合的特異性。
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