induce mutation 中文意思是什麼
induce mutation
解釋
誘導突變-
Environmental mutagens can also induce mutations at undamaged sites and result in the so - called nontargeted mutation
環境誘變劑引起的突變也可發生在非dna損傷部位，我們稱之為非定標性突變（ nontargetedmutagenesis ） 。 -
In our laboratory, a unique mutation detection system using a shuttle vector plasmid has been established to demonstrate that a low concentration of mnng ( 0. 2 m ) can induce nontargeted mutation in mammalian cells : the mammalian cells were exposed to 0. 2m mnng for 2. 5h, then a shuttle plasmid pz189 carrying supf trna gene was transfected into cells after 24h culture. we found a 5 - fold higher mutation frequency of the plasmid replicated in pretreated cells than the spontaneous mutation frequency of the plasmid replicated in control cells. this kind of mutation did not occur immediately after mnng exposure
我們實驗室曾用一特殊的突變檢測系統，直接證明dna損傷劑可在哺乳動物細胞誘發非定標性突變：首先用低濃度（ 0 . 2 m ）的短壽烷化劑mnng （半壽期為1 . 1hr ）處理細胞2 . 5h后，繼續培養24h ，將重組有用作突變檢測的靶基因supftrna基因的穿梭質粒pz189轉入細胞復制，發現在未受致癌物直接攻擊的穿梭質粒中有較自發突變率高5倍以上的靶基因突變。 -
Exponential phase cultures of the dr rl strain survive exposure to gamma radiation at doses as high as 5, 000 gy without loss of viability or evidence of dna damage induced mutation. 6000 gy of irradiation will induce approximately 200 dna double - strand breaks in dr chromosomes, but it is still able to reconstruct a functional genome from chromosomal fragments without any mutation
指數生長期的r1菌株即使在5kgy劑量的-射線照射后，其生長能力也未受影響， 6kgy -射線照射后染色體基因組產生約200個的雙鏈斷裂碎片，但是其基因組dna經修復后沒有引起任何的突變。
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